GENOME ANNOUNCEMENT
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*Streptococcus thermophilus* is a homofermentative thermophilic lactic acid bacterium (LAB) used worldwide as a starter culture for the manufacture of a variety of fermented dairy products ([@B1]). To date, the complete genomes at the chromosome level of *S. thermophilus* strains CNRZ1066 ([@B2]), JIM8232 ([@B3]), LMD-9 ([@B4]), LMG18311 ([@B2]), MN-ZLW-002 ([@B5]), and ND03 ([@B6]) have been published. However, *S. thermophilus* TH1435 and TH1436 represent the first cases of *S. thermophilus* strains isolated in Italy and the only ones from goat milk.

*S. thermophilus* TH1435 and TH1436, collected from two alpine huts (malghe) from raw goat milk used for the artisanal production of Italian cheese, are capable of rapidly lowering the pH of milk, which represents a key technological feature for the dairy industry. Moreover, strain TH1436 is capable of utilizing galactose, while TH1435 lacks this feature.

Whole-genome sequencing of strains TH1435 and TH1436 was performed with an Illumina MiSeq sequencer at the Ramaciotti Centre, Sydney, Australia. Genomic libraries were prepared using the Nextera XT kit Illumina (Illumina, Inc., San Diego, CA), which produced a mean insert size between 800 and 1,200 bp. A total of 776,373 and 1,527,857 paired-end reads (2 × 250 bp) were generated and gave 134- and 159-fold coverages of the TH1435 and TH1436 genomes, respectively. Approximately 85% of these reads were assembled into 36 and 28 large scaffolds, respectively, using a manually curated consensus of assemblies obtained using version 1.2.10 of the Velvet software ([@B7]) and version 2.8 of the 454 Newbler Assembler (454 Life Sciences, Branford, CT). The draft genome of *S. thermophilus* TH1435 is a single circular chromosome of 1,750,348 bases in length, with a mean G+C content of 38.9% and two putative plasmids individuated by BLAST match (scaffolds 33 and 35). The draft genome of strain TH1436 is a single circular chromosome of 1,780,473 bases, with a mean G+C content of 39.0%; no plasmid sequences were detected for this strain.

Genome annotation was performed using RAST ([@B8]) and by the NCBI Prokaryotic Genomes Annotation Pipeline ([@B9]). A total of 1,925 coding sequences (CDSs) and 47 structural RNAs were predicted in strain TH1435, while 1,899 CDSs and 48 RNAs were found in strain TH1436. Additionally, several phage-associated sequences were identified, 12 genes related to transposases and 7 clusters of regularly interspaced short palindromic repeats (CRISPRs) in both strains.

A comparison against the complete genome of *S. thermophilus* CNRZ1066 revealed that TH1435 and TH1436 contain 38 common predicted CDSs that are absent in the reference strain. Furthermore, 31 CDSs specific to TH1435 and 18 specific to TH1436 are present. The sequences of particular interest include a unique gene cluster for the biosynthesis of bacteriocins, resistance to antibiotics, and toxic compounds, as well as several gene clusters that indicate that TH1435 harbors at least one prophage.

Nucleotide sequence accession numbers. {#h1}
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The data from these whole-genome shotgun projects were deposited in GenBank under the accession no. [AYSG00000000](http://www.ncbi.nlm.nih.gov/nuccore?term=AYSG00000000) for *S. thermophilus* TH1435 and [AYTT00000000](http://www.ncbi.nlm.nih.gov/nuccore?term=AYTT00000000) for *S. thermophilus* TH1436. The versions described in this paper are versions AYSG01000000 and AYTT01000000, respectively.

**Citation** Treu L, Vendramin V, Bovo B, Campanaro S, Corich V, Giacomini A. 2014. Whole-genome sequences of *Streptococcus thermophilus* strains TH1435 and TH1436, isolated from raw goat milk. Genome Announc. 2(1):e01129-13. doi:10.1128/genomeA.01129-13.

This study was supported in part by POR Veneto "Fondo Europeo di Sviluppo Regionale" 2007--2013---Asse 1, Azione 1.1.2.

We thank Angiolella Lombardi for providing the *S. thermophilus* strains.
