GENOME ANNOUNCEMENT {#h0.0}
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Polychlorinated biphenyls (PCBs) have become serious and global environmental contaminants because these compounds were widely used for a variety of industrial purposes due to their chemical and physical stabilities. The biphenyl-utilizing bacteria cometabolize certain PCBs into chlorobenzoic acids using biphenyl-catabolic enzymes. However, the biodegradability of PCBs is highly dependent on chlorine substitutions, and the degradation capabilities of PCBs are strain-dependent. *Pseudomonas stutzeri* KF716 was isolated together with more than 10 bacterial strains (KF strains) from the soil near a biphenyl manufacturing plant in Kitakyushu, Japan, by enrichment culture with biphenyl ([@B1]). A 90-kb DNA region containing both the biphenyl/PCB catabolic *bph* gens and salicylate catabolic *sal* genes (termed the *bph-sal* element) was isolated from the *Pseudomonas putida* KF715 genomic cosmid libraries ([@B2]). The *bph-sal* element can be highly transferred by conjugation to various *Pseudomonas putida* strains ([@B1], [@B2]). Therefore, these KF strains could be adequate materials to clarify the molecular mechanisms of adaptive evolution for xenobiotics. We recently began working on the whole-genome sequencing of a series of KF strains ([@B3][@B4][@B9]) Here, we present the genomic feature of *P. stutzeri* KF716.

The draft genome sequence was determined by the National Institute of Technology and Evaluation (NITE) using a strategy combining the 454 GS FLX+ system (Roche) and the MiSeq paired-end sequencing system (Illumina). The reads obtained by the two systems were assembled using Newbler version 2.6 (Roche). The assembled genome is composed of 30 contigs (\>913 bp) totaling 4,188,013 bp, with a G+C content of 64.2%. The *N*~50~ contig size and the largest contig size are 370,448 bp and 655,983 bp, respectively.

The draft genome sequence of the KF716 strain was annotated using RAST version 2.0 ([@B10]) and contains 5,792 predicted coding DNA sequences (CDSs), 3 rRNAs (5S, 16S and 23S), and 50 tRNA sequences. The coding sequences were classified into 2,965 subsystems, the most abundant of which were metabolism of amino acids derivatives (*n* = 314 CDSs) and carbohydrates (*n* = 273); cofactors, vitamins, prosthetic groups, and pigments (*n* = 258); and protein metabolism (*n* = 250). A comparison of genome sequences available in the RAST data sets revealed that *P. stutzeri* A1501 ([@B11]) is the closest neighbor of the KF716 strain with a score of 534, followed by *P. stuzeri* XLDN-R with a score of 462 ([@B12]). The *bph-sal* element, similar to that of *P. putida* KF715 identified previously ([@B2]), was found in a single contig. These data suggest that the *bph-sal* element is mobilizable between the KF strains in the environment and contributes to their genome evolution.

Nucleotide sequence accession numbers. {#s1}
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The draft genome sequence of *P. stutzeri* KF716 has been deposited in DDBJ/EMBL/GenBank under the accession numbers [BBQQ01000001](BBQQ01000001) to [BBQQ01000030](BBQQ01000030).

**Citation** Hirose J, Yamazoe A, Hosoyama A, Kimura N, Suenaga H, Watanabe T, Fujihara H, Futagami T, Goto M, Furukawa K. 2015. Draft genome sequence of the polychlorinated biphenyl-degrading bacterium *Pseudomonas stutzeri* KF716 (NBRC 110668). Genome Announc 3(5):e01215-15. doi:10.1128/genomeA.01215-15.

This work was performed as part of a project supported by the Ministry of Economy, Trade and Industry of Japan.
