Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application

Abreviews

Notes

Flow Cyt

Use at an assay dependent concentration. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC/IF

Use at an assay dependent concentration.

Microcytotoxicity testing

1/10.

Functional Studies

Use at an assay dependent concentration. Cell Typing and Chimerism Studies.

Target

RelevanceHLA A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane.
Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa.