Administration of β-Glucan Drives Expansion of HSPC Subpopulations WT mice were injected with β-glucan or PBS, and BM analysis was performed after 24 hr. (A) Representative fluorescence-activated cell sorting (FACS) plots for the identification of hematopoietic progenitor cells. After gating for Lin − cells, LSK cells were characterized as cKit + Sca1 + cells. LSK cells subpopulations were further characterized as MPP (CD48 + CD150 − LSK), ST-HSC (CD48 − CD150 − LSK), and LT-HSC (CD48 − CD150 + LSK). (B and C) Cell numbers of LSKs, MPPs, ST-HSCs, and LT-HSCs (B) and cell percentages of the same populations in total BM cells (C) of mice at 24 hr after the administration of PBS or β-glucan (ns = 11 and 12 mice). (D) Representative FACS plots for the identification of MPP subpopulations. After gating for LSK cells, MPP4 cells are characterized as CD48 + Flt3 + CD150 − LSK, MPP3 cells are characterized as CD48 + Flt3 − CD150 − LSK, and MPP2 cells are characterized as CD48 + Flt3 − CD150 + LSK. (E) Frequency of MPP subpopulations in LSK cells in the BM of mice at 24 hr after the administration of PBS or β-glucan (n = 5 mice per group). (F and G) Representative FACS plots for the identification of CD41 + LT-HSCs (F) and frequency of CD41 + LT-HSCs (in total LT-HSCs) (G) in the BM of mice at 24 hr after the administration of PBS or β-glucan (n = 5 mice per group). Data are presented as mean ± SEM. ∗ p