Quantitation of A431 IZ cells and A431 EWI-2 cell spreading assays on laminin-5. The areas of 20–45 cells per coverslip were measured using Scion Image v1.62 software. Specific spreading was calculated by subtracting the mean area of cells fixed immediately after plating from the mean area of cells fixed at the end of the assay. Error bars indicate the SEM for each coverslip. Two coverslips per condition were examined; results for each coverslip are shown individually. Asterisk Indicates statistically significant difference between antibody-treated cells and the coverslips in the no antibody controls (P