Plasmid hAng or RNH1 overexpression in PC12 and their impact on tRNA cleavage after stress. A: western blotting confirmation of Flag labeled human angiogenin expression in PC12. B: Western blotting confirmation of RNH1 overexpression after plasmid induction. RNH1 expression increased 2.5 folds when corrected to beta-actin expression (graph represent relative quantification using ImageJ). C: SYBR gold staining after hAng overexpression and stress shows that hAng overexpression increased tRNA cleavage after As stress only and had not impact on A.M stress. D: RNH1 overexpression did not suppress tRNA cleavage after As stress. Arrow: tRNA, dotted line: tiRNA.