The relaxation of smooth muscle is associated with a reduction in free intracellular calcium ion concentration. The effect of the reduction is
Due to relaxation of smooth muscle, dephosphorylation of myosin by myosin light chain phosphatase.
Reestablishment of inhibition of actin-myosin interaction
Deactivation of enzymatic activity of the individual actin molecules
Decreased phosphorylation of myosin molecules
Reduced contractile interaction by blocking the active sites of the myosin molecules