Which method is used to locate a known gene locus?
Ans. A. FISH(Ref: Robbins 9/e page 177)Robbins 9th edition gives the following descriptionFISH uses DNA probes that recognize sequences specific to particular chromosomal regions.MethodRequires growing cellsDetects deletion and duplicationDetects balanced structural rearrangementsDetects uniparental disomyLower limits of detectionG bandingYesYesYesNo5-10mbMetaphase FISHYesYesYesNo40-250 thousand mbInterphase FISHNoYesSomeNo40-250 thousand mbCGH arrayNoYesNoNoSingle Exon or Single geneSNP arrayNoYesNoSomeSingle Exon or Single gene
FISH
CGH
Chromosome painting
RT-PCR
Molecular Biology Techniques