Large dose of EDTA are used in carbonic anhydrase enzyme inactivation. The mechanism by which EDTA act is -
Ans. is 'a' i.e., It chelates with metal ions of the enzyme o EDTA is a chelating agent and it has already been explained that chelating agent has legands (reactive groups) for metal binding. o These ligands of chelating agent bind to metal ions and cause chelation. o EDTA binds to zinc ion of carbonic anhydrase and this results in formation of ternary complex involving the enzyme, chelating agent and metal ions. The formation of ternary complex results in inactivation of enzyme.
It chelates with the metal ions of the enzyme
It combines with the substrate and reacts with the enzyme
Combines with the substrate and doesn't react with the enzyme
Enzyme-EDTA complex can not be attached to substrate