All are true about nohern blotting technique except
The nohern blot is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample. A general blotting procedure stas with extraction of total RNA from a homogenized tissue sample or from cells. Eukaryotic mRNA can then be isolated through the use of oligo (dT) cellulose chromatography to isolate only those RNAs with a poly(A) tail.RNA samples are then separated by gel electrophoresis. Since the gels are fragile and the probes are unable to enter the matrix, the RNA samples, now separated by size, are transferred to a nylon membrane through a capillary or vacuum blotting system. Capillary blotting system setup for the transfer of RNA from an electrophoresis gel to a blotting membrane.
Involves electrophoresis
Requires hybridization probes
Used to detect DNA molecules
Requires restriction endonucleases
Enzymes