The most widely used method for determining the purity of a protein is?
The most widely used method for determining the purity of a protein is polyacrylamide gel electrophoresis (PAGE) in the presence of an anionic detergent, sodium dodecyl sulphate (SDS). Electrophoresis separates the charged biomolecules based on the rates at which they migrate in an electrical field. Reference: Harpers illustrated biochemistry 30th edition
Isoelectric focusing
Polyacrylamide gel electrophoresis
Ion exchange chromatography
High pressure chromatography
miscellaneous