Which of the following is not true about eukaryotic DNA ligase?
NAD+ is the energy source for Prokaryotic DNA Ligase ATP is the energy source for Eukaryotic DNA Ligase Role of DNA Ligase: Sealing the single stranded nick during replication and repair. In Recombinant DNA technology, ligase is used to join two different DNA molecules. Enzyme Function Helicases Unwinding of DNA DNA Polymerase III Polymerization of deoxynucleotides Topoisomerases Cut and reseal DNA, Relieve positive supercoils, Don't use ATP Single Stranded Binding proteins (SSBs) Prevents reannealing or rejoining of two seperate strands DNA Primase Initiates synthesis of RNA primers DNA Ligase Creates 3'5' phosphodiester bond and seals nick Okazaki fragments on lagging strand by using ATP
Catalyses the formation of phosphodiester bond
NAD+ is energy source
ATP is the energy source
Can act only on dsDNA
Replication