Carboxylases requires
Pyruvate in the cytoplasm enters the mitochondria. Then, carboxylation of pyruvate to oxaloacetate is catalyzed by a mitochondrial enzyme, pyruvate carboxylase. It needs the co-enzymes biotin and ATP.Ref: DM Vasudevan, page no: 103
Vitamin B12
Folic acid
Niacin
Biotin
Enzymes