DNA fragments formed by the action of Restriction Endonucleases, are separated by:
B i.e. Agarose gel electrophoresis - Restriction endonuclease (restriction enzyme) makes 2 incisions through sugar phosphate backbone (phospho diester bond) of each strand of ds DNAQ at specific sequence k/a recognition sequences or restriction site. So RE restricts viral (bacteriophage - DNA) replicationQ and protects bacteria (prokaryote) from infection by virusQ. - RE cuts both strands of double stranded (ds) DNA at specific restriction sites with palindromic (inverse repeat) arrangement; thus producing smaller, manageable fragments with sho sequenes and sticky/ blunt endsQ. These restriction fragments can be isolated by agarose gel /polyacrylamide - electrophoresisQ or HPLC. After digestion by restriction endonucleases the DNA ends can be ligated (joined/ annealed) by ' DNA-ligaseQ
Gel electrophoresis
Agarose gel eletrophoresis
Paper Chromatography
High pressure liquid chromatography