In molecular cloning, Blue-white screening is used for
The blue-white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. DNA of interest is ligated into a vector.The vector is then inseed into a competent host cellble for transformation, which are then grown in the presence of X-gal.Cells transformed with vectors containing recombinant DNA will produce white colonies; cells transformed with non-recombinant plasmids (i.e. only the vector) grow into blue colonies.This method of screening is usually performed using a suitable bacterial strain, but other organisms such as yeast may also be used.DM VASUDEVAN 7th edition pg no 626 627
To screen for recombinant vectors
To detect gene mutations
To identify desired chromosal DNA inse in plasmid vectors
To detect host DNA in situ
Metabolism of nucleic acids