Which of the following technique is used to find out the impoant amino acid residue involved in the enzyme catalysis?
Codon for specific aminoacids can be altered using site-directed mutagenesis. Site-directed mutagenesis Identification of the specific roles of given aminoacyl residues in substrate binding and catalysis. For eg : A paicular aminoacyl residue functions as a general acid can be tested by replacing it with an aminoacyl residue incapable of donating a proton.
Electrophoresis
Chromatography
Mass spectrometry
Site directed mutagenesis
Techniques in molecular biology