A surgeon suspecting testicular carcinoma in a patient asks the intern to send the sample for histopathology, what is the fluid in which the intern should send the sample to the pathologist?
Ans. (b) 10% formalin fixative-for-testis-biopsy.html(Ref: Complete review of pathology 2nd ed/Annexure 4. )This is a tricky Question !When you first see the term analysis for sperm, the answer is bouin s fluid. But what the examiner wants to know is whether you know that for histological diagnosis, the fixative is 10% formalinRemember: bouins fluid should be used for testicular anlysis in cases of infertility or even CIS (carcinoma in situ) as Formalin induces marked shrinkage - difficulty in germ cell and CIS recognition.But once you have known case of testicular carcinoma, then you need to make correct diagnosis on the basis of morphology, also preserve antigens for IHC and do molecular tests, which comes best when you fix the tissue in 10% buffered neutral formalin
Bouvin solution
10% formalin
95% ethanol
Alcohol
Male Genital Tract