RFLP is used for
Reastiction fragment length polymophism (RFLP) : A polymorphic gene is one ,in which the variant alleles are commonin more than 1% of the total population. The existence of two or more types of restriction fragment patterns is called restiction fragment length polymorphism (RFLP). This can be used as a genetic marker. DNA is treated with restriction enzymes, which cleave DNA into fragments of lengths. The electrophoresis is done in agarose gels, when the fragments are seperated. Finally, the DNA from the agarose gel is transferred on to nitrocellulose paper (southern blotting)and hybridized with labeled probe sequences. Genotypic changescan be recognized by the altered restriction fragments. REF : DM VASUDEVAN TEXTBOOK ,7th EDITION , Page no no :637.
Analysis of chromosomal structures
DNA estimation
Synthesis of nucleic acid
Detecting proteins in a cell
Metabolism of nucleic acids