Kary otyping is done in which phase of cell cycle-
Ans. is 'b' i.e., Metaphase o To produce a karyotype, one must obtain cells capable of growth and division as karyotyping is done by arresting mitosis in dividing cells in metaphase.o Karyotyping is the study of chromosomes and is used in cytogenetics to study the chromosomal abnormalities,o After arresting the cells in metaphase the chromosomes are examined to see for:i) Numbers - to detect abnormalities in chromosome numbers such as aneuploidy (trisomy, tetrasomy), polyploidy,ii) Structure - to detect structural chromosomal anomalies such as translocations, deletions, inversions.Karyotypingo It is the study of chromosomes,o It is used in cytogenetics to study the chromosomal abnormalities.o A karyotype is a standard arrangement of a photographed or imaged stained metaphase spread in which chromosome pairs are arranged in order of decreasing length.o To produce a karyotype, one must obtain cells capable of growth and division as karyotyping is done by arresting mitosis in dividing cells in metaphase,o Samples used are : -Amniotic fluids (cells from amnion, skin, urogenital system, respiratory system and GI system).# Chorionic vill.# Bone marrow# Skin fibroblasts# Peripheral blood lymphocytes# Fetal umblical blood.# Lymph node tissue# Solid tumor sample
Anaphase
Metaphase
Telophase
S phase
Cytogenetic Disorders