Steps of PCR in sequence are ?
PCR - Method of amplifying a target sequence of DNA developed by Karry Mullis in 1984 Essential requirements of a PCR reaction: Targeted DNA Two primers Four deoxyribonucleotide Thermostable DNA polymerase Steps of PCR: 1. Denaturation: Two DNA strands separated (94o C) 2. Annealing: Addition of primers (one for each strand) (60oC) 3. Extension/Elongation: Polymerization or synthesis of DNA. (72oC) These cycles of Denaturation, Annealing, and Extension are repeated again and again to synthesize large amounts of DNA.
Extend DNA, Anneal Primers, Denature DNA
Anneal Primers, Extend DNA, Denature DNA
Denature DNA, Anneal Primers, Extend DNA
Denature DNA, Extend DNA, Anneal Primers
Techniques in molecular biology