Which of the following is used for sequencing a long DNA fragment :
Chromosome walking Sequencing method of choice for sequencing long DNA fragments between 1.3 and 7 kb Applications of chromosome walking: Positional cloning Find, isolate, and clone a paicular allele Isolate a locus of interest for which no probe is available Analysis of genetically transmitted diseases Detect mutations Study SNPs (Single Nucleotide Polymorphisms) Disadvantage : Speed of this technique can't be enhanced
Sanger's technique
Chain termination method
Chromosome Walking
RFLP
Techniques in molecular biology