Oil red o staining used for
Ref Bancroft histology 7/e p 83 Air dry frozen sections on slides for 30 minutes minimum 2. Fix in 10% neutral buffered formalin for 10 minutes 3. Dip in 60% isopropanol 1 time quickly 4. Stain in working Oil Red O solution for 15 minutes 5.
Frozen section
Glutaraldehyde fixed specimen
Alcohol fixed specimen
Formalin fixed specimen
General anatomy