What do restriction endonucleases do?
Restiction Endonucleases (RE) : In order to transfer a gene, it is to be first selectively split from the parent DNA. It is usually achieved by the restriction endonucleases. RE have specific recognition sites where they cut the DNA. There are >800 such enzymes now available commercially. These enzyms recognize specific sequence with palindrome arrangement. REF :DM VASUDEVAN textbook; 8th EDITION; Page no :601
Cut ds DNA at specific sites
Cut RNA at specific sites
Cut ss DNA at specific sites
Break peptide chains
Metabolism of nucleic acids