Which of the following structure is not affected by protein denaturation?
PROTEIN DENATURATION • In quaternary structure denaturation, protein sub-units are dissociated and/or the spatial arrangement of protein subunits is disrupted.
• Primary structure, such as the sequence of amino acids held together by covalent peptide bonds, is not disrupted by denaturation.
• In secondary structure, denaturation proteins adopt a random coil as they lose all regular repeating patterns such as alpha-helices and beta-pleated sheets and configuration.
• Tertiary structure denaturation involves the disruption of:
1. Covalent interactions between amino acid side- chains (such as disulfide bridges between cysteine groups)
2. Non-covalent dipole-dipole interactions between polar amino acid side-chains (and the surrounding solvent)
3. Van der Waals (induced dipole) interactions between nonpolar amino acid side-chains
Primary structure
Secondary structure
Tertiary structure
Quaternary structure