luorescence is used in assessment of levels of which hormone commonly -
(A) (Thyroid) (Internet)Fluoroscopy is used in assessment of all the hormones.Fluorescence spectroscopy aka fluorometry or spectrofluorometry, is a type of electromagnetic spectroscopy which analyzes fluoescence from a sample. It involves using a beam of light, usually ultraviolet light that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily, visible light. A complementary technique isabsorption spectroscopy.Although the RIA technique is extremely sensitive and extremely specific, requiring specialized equipment, it remains among the least expensive methods to perform such measurements. It requires special precaution and licensing, since radioactive substances are used. The unique ability of RIA to measure small molecules can nowadays be achieved in many cases by non-radioactive methods such as ELISA, where the antigen-antibody reaction is measured using colorimetric, such as absorbance, fluorescence intensity or polarization. This, combined with the usual sandwich configuration of ELISA requiring two domains of an analyte to be present to generate a signal, has led to many assays being developed for ELISA instead of RIA or for RIA assays to be replaced by ELISA.
Thyroid
Steroid
Catecholamines
Leutenising releasing hormone
Endocrinology