The following technique is used to analyze DNA obtained from cancer biopsies, in which tumor cells are often contaminated with large numbers of admixed stromal cells
Ans. (c) Pyrosequencing(Ref: R 9th/pg 140; 8th/pg 140)Pyrosequencing:Highly sensitive than Sanger sequencing and can detect even 5% mutated DNAFor this reason, it is be used to analyze DNA obtained from cancer biopsies, in which tumor cells are often "contaminated" with large numbers of admixed stromal cells.
Sanger sequencing
Single base primer extension
Pyrosequencing
Amplicon length analysis
Genetics