The laboratory diagnosis of thiamine deficiency is by:
The laboratory diagnosis of thiamine deficiency usually is made by a functional enzymatic assay of transketolase activity measured before and after the addition of thiamine pyrophosphate. >25% stimulation by the addition of thiamine pyrophosphate is interpreted as abnormal. Thiamine or the phosphorylated esters of thiamine in serum or blood also can be measured by high-performance liquid chromatography to detect deficiency.
Transaldolase assay measured before and after the addition of thiamine pyrophosphate
Transketolase assay measured before and after the addition of thiamine pyrophosphate
Thiamine pyrophosphate assay in RBC after adding sugar
Serum LDH levels