A patient is having thick, gray coating on the throat and tonsils, followed with fever, chills and swollen glands in the neck. Microscopic examination of nasopharyngeal or pharyngeal swab showed gram positive organism with a special stain. The constitutes of the stain are:-
Gram positive organism causing gray colored coating (pseudomembrane) is Corynebacterium diphtheriae . So the specific stain is Albe stain. Contents of Albe stain:-Stain A- glacial acetic acid, malachite green ,toluidine blueStain B- KI + I2
Crystal violet. Gram's iodine
Toluidine blue, malachite green, glacial acetic
Carbol fuchsin, acid alcohol, and methylene blue
Methylene blue
Systemic Bacteriology (Gram Positive Bacilli, Gram Negative Bacilli)