The comparison of the amino acid sequence of Cytochrome C from different species shows many variations. Most of these variations are found:
Most variations in aminoacid sequence of cytochrome c affect primary structure in a way not to affect the secondary and teiary structures which are more impoant for function.The amino acids that cannot be replaced without a major modification of the enzymic propeies of the cytochrome c are not altered.Thus the amino acids at locations that determine the secondary structure of protein (loops, helix or strands) are preserved while aminoacids that do not contribute to the determination of secondary and teiary structure are preferentially altered.These alterations are likely to be distributed randomly as they result from point mutations on the responsible gene and this is the single best answer of exclusion here.
Randomly
Only in helical regions
Only in strand regions
Mainly in loop regions