Larger DNA segments can be cloned in
Hybrid DNA molecules can be constructed in cloning vectors typically using bacterial plasmids, phages, or cosmids. A clone is a large population of identical molecules, bacteria, or cells that arise from a common ancestor. Molecular cloning allows for the production of a large number of identical DNA molecules, which can then be characterized or used for other purposes. Cloning capacities of vectors Plasmid 0.01-10 kb Lambda phage 10-20 kb Cosmids 35-50 kb Bacterial aificial chromosome 50-250 kb Yeast aificial chromosome 500-3000 kb
Plasmids
Bacteriophage
Cosmids
Bacterial Aificial Chromosomes
Techniques in molecular biology