In determining protein structure, mercaptoethanol is used to:
Ans. C. Reduce disulfide bondsb-mercaptoethanol reduces disulfide bonds. Identification of C-terminus is by Carboxypeptidase C or Y which cleaves any C-terminal amino acid, carboxypeptidase B cleaves only Arg or Lys, carboxypeptidase A does not cleave Arg, Lys, or Pro. Denaturation is disruption of protein structure by adding chemicals or acidic or alkaline reagents, heat etc.
Identify C-terminal residue
Denature protein
Reduce disulfide bonds
Oxidize disulfide bonds
Structure & Function of Protein