The first step in fatty acid synthesis involves
Acetyl CoA carboxylase 

Production of malonyl-CoA from acetyl-CoA is the initial and rate limiting step in fatty acid synthesis. The reaction is catalyzed by acetyl-CoA carboxylase.
Acetyl CoA carboxylase
b-Hydroxyl CoA dehydrogenase
Acetyl dehydrogenase
Pyruvate kinase