Various biomedical tools are used for DNA technology. Which test uses oligomer with single base pair substitution?
An inherited difference in the pattern of restriction enzyme digestion (eg, a DNA variation occurring in more than 1% of the general population) is known as a restriction fragment length polymorphism (RFLP). RFLPs result from single-base changes (eg, sickle-cell disease) or from deletions or inseions (CNVs) of DNA into a restriction fragment (eg, the thalassemias) and have proved to be useful diagnostic tools. Ref: Weil P. (2011). Chapter 39. Molecular Genetics, Recombinant DNA, & Genomic Technology. In D.A. Bender, K.M. Botham, P.A. Weil, P.J. Kennelly, R.K. Murray, V.W. Rodwell (Eds), Harper's Illustrated Biochemistry, 29e.
PCR
RFLP
Error coded mutation analysis
FISH