Sections from a solid-cytic unilateral ovarian tumor in a 30-year old female show a tumor composed of diffuse sheets of small cells with doubtful nuclear grooving and scanty cytoplasm. No Call-Exner bodies are seen.The ideal immunohistochemistry panel would include
Tissue was fixed using 10% buffered formalin solution. Five-micrometer sections of formalin fixed paraffin-embedded specimens were cut and immunohistochemical staining was performed using the peroxidase anti-peroxidase method. Fresh citric buffer solution of pH 6 was used for all markers except estrogen receptor (ER) and Wilms' tumor 1 (WT1) (pH 2.5). Antigen retrieval was carried out using a microwave oven. Next a peroxide block was used to prevent background non-specific staining due to endogenous peroxidases. This was followed by the addition of the primary and secondary antibodies and subsequently the addition of the chromogen, 3,3Diaminobenzidene. Slides were counterstained using H and E, stain. We used a panel of six markers including cytokeratin7 (CK7), CK20, carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), ER and WT1 to differentiate between primary and metastatic ovarian tumors
Vimentin, epithelial membrane antigen, inhibin, CD99
Desmin, S- 100 protein, smooth muscle antigen, cytokeratin
Chromogranin, CD45, CD99, CD2O
CD3, chromagranin, CD 45, synaptophysin
miscellaneous