Hybridoma used in:
ln 1975, George Kohler and Cesar Milstein created hybrid cells that will make unlimited quantities of antibodies with defined specificities, which are termed as monoclonal antibodies . This discovery know as hybridoma technology Principle - fusion between myeloma cell (malignant plasma cell) and spleen cell from a immunized animal. Hybridoma technology can make available highly specific antibodies in abundant amounts. use of monoclonal antibodies Example of monoclonal ab trastuzumab - EGRF/HER2 INHIBITORS BEVACIZUMAB - ANGIOGENESIS INHIBITORS
In situ hybridization
Sequencing DNA
Production of monoclonal antibodies
Production of continuous cell lines
DNB 2018