A Giemsa stain of a thin peripheral blood smear is prepared. Which of the following cannot be diagnosed?
Ans. (B) Coxiella burnettii(Ref: Jawetz's Medical Microbiology 27th Ed; Page No-347)A diagnosis of Coxiella burnettii cannot be done using a Giemsa stain of a thin peripheral smear.The diagnosis of Coxiella burnetii is done mainly by serology which shows a rise in the titer of specific antibodies to C. burnettii.Other Options:Toxoplasma gondii: It can invade any nucleated cell and replicate within cytoplasmic vacuoles by a process called endogony (internal budding). The trophozoites stain with Giemsa stain.Bartonella henselae: Invade macrophages and neutrophils which can be seen by Giemsa staining of a peripheral smear.Ehrlichia chaffeensis: They infect circulating leukocytes, erythrocytes and platelets, where they multiply within phagocytic vacuoles, forming clusters known as morulae. They can be seen on peripheral blood smear using Giemsa stain.
Bartonella henselae
Coxiella burnettii
Toxoplasma gondii
Ehrlichia chaffeensis
Bacteria