All the following can be used to detect the mutation, except
Methods used for detecting mutations 

Cytogenetics analysis  
FISH   
PCR, 
RFLP 
DNA sequencing, Southern blotting 
Microarrays 
Single-stranded conformational polymorphism (SSCP)
RNAase cleavage
Denaturating gradient gel electrophoresis (DGGE)
Protein truncation test
Single strand conformational polymorphism
Ligase chain reaction
Polymerase chain reaction
DNA Sequencing