Steps of Gram stain sequence arrangement. a. Mordant- iodine treatment b. Safranin c. Crystal violet d. Alcohol wash
Steps of Gram stain sequence arrangement. Step Name Feature 1 Primary stain Stain with crystal violet for 1 minute and rinse with water 2 Mordant Stain with gram's iodine for 1 min 3 Decolorization Acetone for 2-3 s or 95% ethanol for 20-30s 4 Counter stain Safranin for 30-60s
a>b>c>d
b>c>a>d
c>a>d>b
b>a>d>c
General Microbiology Pa 1 (History, Microscopy, Stains and Structure and Physiology of Bacteria)