In vitro feilization is indicated in :
Ans. is a i.e. Tubal pathology IVF was first developed as a means to over come infeility resulting from irreparable tubal disease as feilization takes place in fallopian tube. Now the spectrum of IVF has broadened and is indicated in number of conditions including : Tubal pathology resulting from previous infection or advanced stages of endometriosis or in cases with proximal and distal obstruction.deg In women desiring reversal of tubal sterilization with poor prognosis for recanalization.deg Male factor infeility : But if sperm counts are less than 0.5 x 106/m1 it is associated with poor feilization rates with IVF (IVF is not indicated in azoospermia).deg Multifactor infeilitydeg Unexplained infeilitydeg Immunological factor in males.deg Ovarian failure and Diminished ovarian reserve : here IVF is performed using donor oocvtes.deg Women recently diagnosed with a cancer or another medical disorder facing eminent treatment (chemotherapy and radiotherapy) that poses a serious threat to their future feility. Such women can have cryopreservation of their embryos and later IVF done.deg Women with normal ovaries but no functional uterus as a result of a congenital anomaly (mullerian agenesis), advanced disease (multiple myeloma, severe intrauterine adhesions) or a previous hysterectomy can be afforded the oppounity to have their own genetic offspring IVF with transfer of embryos to the uterus of a surrogate.deg Women who carry genetic risk or disorder which may be expressed in their offspring can be the candidates for IVF with preimplantation genetics to identify and exclude affected embryos.deg Since. we have to choose one option Tubal pathology i.e. option 'a' is the best option.deg Technique of IVF : Sperms are retrieved using various techniques and prepared by wash and swim up technique. Oocyte is retrieved by vaginal needle aspiration 36 hours after HCG administration. 50000 to 100000 sperms are placed in culture media containing oocyte within 4-6 hours of retrieval. They are examined after 12-24 hours and if they show presence of 2nd polar body, feilization has occurred. The feilized ova at 4-8 cell stage is placed into the uterine cavity close to fundus. Not more than three embryos are transferred per cycle.
Tubal Pathology
Uterine dysfunction
Ovarian pathology
Azoospermia