In vivo control of citric cycle is affected by
Regulation of TCA Cycle 1. As the primary function of TCA cycle is to provide energy, respiratory control the ETC and oxidative phosphorylation exes the main control. SECTION FOUR 2. In addition to this overall and coarse control, several enzymes of TCA cycle are also impoant in the regulation. Three Key enzymes are: * Citrate synthase * Isocitrate dehydrogenase (ICD) *aaa-oxoglutarate dehydrogenase These enzymes are responsive to the energy status as expressed by the ATP/ADP ratio and NADH/NAD+ ratio * Citrate synthase enzyme is allosterically inhibited by ATP and long-chain acyl-CoA. * NAD+-dependent mitochondrial isocitrate dehydrogenase (ICD) is activated allosterically by ADP and is inhibited by ATP and NADH. * a-oxoglutarate dehydrogenase regulation is analogous to PDH complex. 3. In addition to above, succinate dehydrogenase enzyme is inhibited by OAA and the availability of OAA is controlled by malate dehydrogenase, which depends on NADH/NAD+ ratio. Most of ATP is formed as a result of oxidative phosphorylation resulting from re-oxidation of reduced coenzymes, viz., NADH and FADH2 by the respiratory chain. The remainder is generated by Phosphorylation at substrate level.Ref: MN Chatterjea Textbook of Medical Biochemistry, 8th Edition, Page no: 342 & 343
Acetyl CoA
Coenzyme A
ATP
Citrate
Respiratory chain