PCR is used to :
Polymerase chain reaction (PCR) - used to amplify small amount of DNA In 1983, Kary Mullis developed a new technique that made it possible to synthesize large quantities of a DNA fragment without cloning it. Basic Steps involved in PCR are: a) DNA extraction from microorganism b) Amplification of extracted DNA- 3 steps -Denaturation (95degC), Primer annealing (55degC), Extension (72degC) c) Post amplification analysis by different methods like Gel electrophoresis.
Detect target plasmids
Amplify small amount of DNA
Seal the cut ends of DNA
Cleave the bacterial plasmid
General Microbiology Pa 1 (History, Microscopy, Stains and Structure and Physiology of Bacteria)