Which of the following statements are true about estimation of prothrombin time (PT)?
In coagulation studies: Most routine coagulation investigations are performed on platelet-poor plasma (PPP). The sample should be kept at room temperature if it is to be used for PT tests, lupus anticoagulant (LAC) or factor VII assays and it should be kept at 4 degC for other assays. The testing should preferably be completed within 2 h of collection. Coagulation studies are of 2 types: PT --> Thromboplastin is used aPTT --> Kaolin/silica is used Anticoagulant of choice for this study is 3.2 % trisodium citrate. Ratio of blood sample to anti-coagulant is 9:1.
Immediate refrigeration to preserve factor bility
Platelet-rich plasma is essential
Done preferably within 2 hours of collection
Utilises Kaolin
Coagulation