Enzyme that can be traced in semen sample of 8 -10 weeks is:
D i.e. CPK enzymeRef: Essentials of Forensic Medicine and Toxicology by Narayan Reddy, 34th editionExplanation:SEMINAL STAIN EXAMINATIONInvisible stainsOn UV light - fluoresces (bluish white on a dark background).False positivesOther body fluids, e.g., blood, pus, saliva etc.Also fabric whiteners.Florence test - For Choline (seminal vesicle)Stain extract + Florence solution (potassium iodide, iodine and water) = dark-brown rhomboid crystals of choline per iodide (larger than haemin crystals).A negative reaction is proof that stain is not seminal.Barberio's test - For Spermin (Prostatic secretion)Stain extract + aqueous or alcoholic soln. of picric acid = yellow, long needle shaped crystals (of spermin picrate).Acid phosphatase test - Highest concentration in seminal fluid20 to 400 times more than any other body fluid.Undiluted semen - 300-360 Bodanksy units.100 B units/ml - indicative of semen.Test is conclusive in the absence of demonstrable sperms or in azoospermia.CPK enzymeSpermatozoa contain a high concentration of CPK, which is more than double than found in any other body fluid.Normal cone. - 385 to 1400 units/ml.400 units/ml - conclusive of seminal stain.Can Be Detected Even In 6 Months Old Stain.Aluminium molybdate testDetects phosphorus.P30 - PSA prostate specific antigenA glycoprotein derived from prostatic epithelial cells.Found in - semen, male urine and blood (only for male).Detected in old and dried stains too.Reliable indicator of semen.Monoclonal antibody mouse anti-human semen-5 (MHS-5)Secreted by seminal vesicle epithelium.Only found in semen.Highly reliable for semen.LDH isoenzyme of spermatozoa (LDH C4)Detected on electrophoresis.Absolute proof of semen.Demonstration of spermatozoaIn livingMotile sperms seen 12 hours after ejaculation into vagina.Complete sperm are seen upto 26 hours (2-3 days).Then separate into head and tail - identifiable, upto 120 hours.In the deadSeen in vagina up to 1 or 2 weeks.Up to a fortnight in uterine cavity.Species identificationPrecipitin test.LDH isoenzyme pattern.Y bodies on human spermatozoa heads.Absolute Proof of SemenOne unbroken spermatozoa.LDH isoenzymes detection.
Acid phosphatase test
ALP test
LDH
CPK enzyme
Sexual Offenses