Real time PCR is used for -
Ans. is 'd' i.e., For quantitative dectection of PCR materialVarious types of PCR1) Multiplex PCR:# To find out the deletion mutation in a patient DNA.# In this type of PCR, multiple pairs of oligonucleotide primer are synthesized to amplify the entire gene or selected region of the gene.# After this, agrose gel electrophoresis reveals any deletion mutation compared to normal gene product.2) Reverse transcriptase PCR:# In this type of PCR, RNA is the starting material on which the reverse transcriptase enzyme acts to convert it to the DNA which is subsequently used for PCR.# DNA polymerase derived from thermus thermophillus organism has got additional reverse transcriptase activity and hence is preferred for reverse transcriptase type of PCR.3) Real time PCR:# In this type of PCR, a fluorescent dye known as "SYBR Green is used to tag the primer, this helps in quantitative detection of PCR material.4) Invert PCR:# In this type of PCR, the flanking region of the DNA is converted to target region and target region is convered to flanking region.# This is done for those DNA fragments for which the sequence of outer flanking region for the construction of primer is not known.5) Nested PCR :# In this type of PCR, specificity of amplification is enhanced by using new set of primer in each phase of the PCR# New set of primer is complimentary to the template DNA sequence which is downstream to the first primer, i.e. nested between the original set of primer.
Multiplication of RNA
Multiplication of Proteins
Multiplication of specific segments of DNA
For quantitative dectection of PCR material
Immunology