If a radioimmunoassay is properly conducted and the amount of radioactive hormone bound to antibody is low, what would this result indicate?
In a radioimmunoassay, there is too little antibody to completely bind the radioactively tagged hormone and the hormone in the fluid (plasma) to be assayed. Thus, there is competition between the labeled and endogenous hormone for binding sites on the antibody. Consequently, if the amount of radioactive hormone bound to antibody is low, this finding would indicate that plasma levels of endogenous hormone are high
Plasma levels of endogenous hormone are high
Plasma levels of endogenous hormone are low
More antibody is needed
Less radioactive hormone is needed
Endocrine System