Which of the following is the best method for monitoring thiamine level in blood?
Thiamine deficiency is diagnosed by an increase in erythrocyte transketolase activity observed upon addition of thiamine pyrophosphate. The activation of apo-transketolase (the enzyme protein) in erythrocyte lysate by thiamine diphosphate added in vitro has become the accepted index of thiamine nutritional status. Ref: Harper's Illustrated Biochemistry, 26th Edition, Pages 488-489 ; Lippincott Biochemistry, 2nd Edition, Page 322 ; Textbook of Biochemistry By D M Vasudevan, 3rd Edition, Page 269
G6PD activity
Reticulocytosis
Absolute thiamine level in blood
Transketolase level in blood