A person has unprotected sex 3 weeks back. To rule out HIV infection the best test is-
Ans. is 'a' i.e., p24 antigen assay Laboratory diagnosis of HIV infectiono Laboratory diagnosis is made by :A) Antibody detection# It is the most common method used for diagnosis of HIV infection.# ELISA is the most sensitive test and therefore is the serening test of choice.# Western blot is the most specific (Confirmatory) test and therefore is the 'gold standard'for diagnosis.# Antibodies test usually become positive 22 days after the infection.B) Detection of HIV nucleic acid or antigen# p24 antigen detection by ELISA can be done 16 days after infection (earlier than antibody detection).# Nucleic acid detection tests are the earliest to become positive (12 days after infection). Most commonly used test is reverse transcriptase PCR, (RT-PCR). Other methods used are DNA-PCR, RNA-PCR, RNA- bDNA (branched DNA), and RNA-NASBA (nuclei acid sequenced based assay).C) Virus isolation# Co-cultivation of the patient's lymphocyte with uninfected lymphocytes in the presence of IL-2# Not suitable as a routine diagnostic procedure.In infected individual HIV can be isolated fromo Bloodo Lymphocyteso Semeno Cervical secretionso Salivao Tearso Urineo Breast milk
P24 antigen assay
ELISA
Western blot
Lymphnode biopsy
Human Immunodeficiency Virus