Which of the following technique for protein separation is based on molecular size?
Gel filtration chromatography is known as molecular sieving.Hyrophilic cross linked gels like acrylamide, agarose ,and dextran(sephadex)are used for seperation of molecules based on their size.Sephadex is widelyb used and the range of seperation is based on the pore size.The gel is porus in nature.The small paicls enter ino the gel and reenter into the another paiclebut the large immunoglobulins cannot enter the pores and sidetrack the gel paicles:so they move rapidly.In sho larger molecules will come out first; while smaller molecules retained in the column. REFERENCE ; DM VASUDEVAN Textbook SEVENTH EDITION Page no: 450
Ion exchange chromatography
Gel filtration chromatography
Affinity chromatography
Hydrophobic interaction chromatography
Structure and function of protein