Among the following methods, the one that is MOST sensitive for glucose estimation is:
Enzymatic method of glucose estimation is highly specific, giving 'true glucose' values. The glucose oxidase (GOD) and peroxidase (POD) method is the one most widely used. Glucose oxidase is very specific; it conves glucose to gluconic acid and hydrogen peroxide. Peroxidase conves the H2O2 into H2O and nascent oxygen. The oxygen oxidises a colourless chromogenic substrate (e.g., oho dianisidine) to a coloured one; the colour intensity is directly propoional to concentration of glucose. Ref: Textbook of biochemistry by D M Vasudevan, 5th edition, Page 104.
GOD POD method
O-Toluidine method
Folin-Wu method
Hexokinase method