Nohern blot test is used for:
B i.e. RNA analysis - Blotting techniques separate fragments on the basis of their mass (size) by agarose or SDS- polyacrylamide gel electrophoresis (SDS-PAGE) and then transfer the pattern to nitrocellulose/nylon paper (blotting or adsorption). Then enzyme/radioactive/or fluorescence labeled probe is added complementing, hybridizing & detecting the desired fragment. Southern blotting, Nohern blotting and Western blotting techniques (Mn - "See No West") are used respectively to analyze DNA, mRNA and proteins sampleQ (sequence of protein synthesis). And the probes used in these techniques are c-DNA, cDNA and antibody respectively. - Antigen-antibody reaction is detected by ELISA (enzyme linked immune sorbent assay) and Western (immuno) blot. ELISA is sensitive and Western blot is confirmatory in AIDS diagnosisQ
DNA analysis
RNA analysis
Analysis of proteins
Enzyme analysis