Chlamydia are isolated by
Isolation of chlamydiae can be done by :Inoculation into yolk sac/embryonated eggs of 6-8 day old chick embryo which may be pretreated by streptomycin or polymyxin B.Inoculation into experimental animals (mice)Tissue/cell culture:Preferred modeCommonly used cell lines are McCoy and HeLa cells.Cell cultures are pretreated by irradiation or chemicals such as 5-iodo-2 deoxyuridines or cycloheximide to enhance replication and detection of inclusion bodies.Pretreatment with DEAE dextran or centrifugation after inoculation promotes contact between chlamydiae paicles and cell monolayer.
Enzyme immune assay
Yolk sac inoculation
Direct immunofluorescence
Microimmunofluorescence
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