A highly specific inhibitor that targets the phosphorylation activity of TFIIH is added to an in vitro transcription reaction. Which one of the following steps is most likely to be affected?
Most likely to be affected by inhibitor that targets the phosphorylation activity of TFIIH is promoter clearance Transcription factor II H has two activities : Helicase Kinase -Unwinds the DNA -Phosphorylates the TFIIH- Promoter clearance.
Binding of RNA polymerase to promoter sequence
Promoter clearance
Recruitment of TFIID
Open promoter complex formation
Transcription