In TCA cycle, citrate is conveed into cis-aconitate by which mechanism?
2nd Step: Formation of Isocitrate, Citrate is isomerized to isocitrate by aconitase (Step 2, Fig.18.2). This reaction is a two-step process. At first, one water molecule is removed from citrate forming cis aconitate; a transient compound with a very sho half-life. Immediately, one water molecule is added to aconitate to form isocitrate. Thus the position of the hydroxyl group is shifted.Ref: DM Vasudevan, 7th edition
Loss of H+
Loss of phosphate
Loss of H2O molecule
Loss of carbon dioxide molecule
Metabolism of carbohydrate