A patient was suffering from hepatorenal syndrome. A urine sample was obtained from the patient and was examined under the microscopy. Which of the following technique is being used to make the organism visible?
Picture is showing spiral shaped bacterium ( having hooks ) suggestive of member of spirochetes Organism causing hepato-renal syndrome is Leptospira interrogans which cause ictero hemorrhagic fever/Weil's disease. Dark field microscopy- reflected light is used instead of transmitted light Dark field condenser with central circular stop illuminates the object with cone of light Light rays falling on object are reflected or scattered on to objective lens- object appears self luminous against dark background
Dark field microscopy
Routine microscopy with negatively stained background
Phase contrast microscopy
Compound light microscopy
Systemic Bacteriology (Haemophilus, Yersinia, Spirochaetes, Ricketssia, Chlamydia, Mycoplasma and Miscellaneous Bacteria)