Which of the following is/are true about PCR except:
Ans: A (Uses....) "Reverse transcriptase- PCR is used for qua notification ofRNA Harper 30th/459, Lippincott 6th/ 483Polymerase Chain ReactionPCR provides a sensitive, selective & extremely rapid means of amplifying a desired sequence of DNA. Twenty cycles provide an amplification'* of lO^S 30 cycles of 109 -Harper 27th/410-llSpecificity* is based on the use of two oligonucleotide primers that hybridize to complementary sequence on opposite strands of DNA & flank the target sequenceDouble stranded DNA can be disrupted by beat or high pH, giving rise to single stranded DNA. The single stranded DNAserves as a template for synthesis of a complementary strand by replicating enzymes, DNA polymeraseEarly PCR reaction used an PS coli DNA polymerase that was destroyed by each heat denaturation cycle. Substitution of a heat-stable DNA polymerase (Taq polymerase) 5 from Thermus aquaticus, obviates this problem & has made possible automation of the reaction, since the polymerase reactions can be run at 70,]CPCR is a cyclic process & cycle contains three steps that involves {Chatterjea & Shinde)Denaturation of DNA duplex (94-98degC)Annealing of primers (37-60oC)Extension of the primers with a polymerase in the presence of dNTP (about 72,:C)Use of PCRIn detection of bacterial infections .retroviral infection & latent virusApplication in forensic medicine(r)in prenatal diagnosis - in diagnosis of inherited diseaseIn cancer detectionQIn transplantationTo study evolutionIn sex determination ofembry-osTo determine viral loadIn DNA sequencingFig,; Steps in one cycle of the polymerase chain reaction.
Uses heat labile DNA polymerase
Uses heat stable DNA polymerase
Is technique for DNA amplification
Used to yield multiple copies of DNA
Immunology