Sterilization of culture media containing serum is by :
Most of the culture media are sterilized by Autoclaving at 121°C for 15 min at 15 lb pressure / inch2
Constituents like serum, trypsin, growth factors, proteins, amino acids, vitamins, hormones, carbohydrates and plant extracts are thermolabile and may decompose during autoclaving. Therefore, these must be sterilised by filtration.
Autoclaving
Micropore filter
Gamma radiation
Centrifugation