The following is not a feature of malignant transformation by culturedc
Ref biology of the cell p357-364 Both normal cells and cancer cells can be cultured in-vitro , however they behave quite differently normal cell: show replicative senescence .is cells pass through limited number of cell division before they decline in vigor and die it may caused by inability to synthesise telomerase
Increased cell density
Increased requirement for growth factors
Alterations of cytoskeleton structure
Loss of anchorage
General anatomy