Substrate concentration at which Vi is half the maximal velocity attainable at a paicular concentration of enzyme is called as
Rate of a reaction or velocity (V) is directly propoional to the enzyme concentration when sufficient substrate is present. The velocity of the reaction is increased propoionately with the concentration of enzyme, provided substrate concentration is unlimited ii. Hence, this propey is made use of in determining the level of a paicular enzyme in plasma, serum or tissues. iii. Known volume of serum is incubated with substrate for a fixed time, the reaction is stopped and product is quantitated (endpoint method). Since the product formed will be propoional to the enzyme concentration, the latter could be assayed.Ref: DM Vasudevan, Page no: 48
Vmax
Vmax/2
Km
Km/2
Enzymes