Which of the following is not antibody dependent methods
Antibody dependent methods are:

EUSA
Protein Immunoprecipitation (ChIP assay)
Immunoelectrophoresis
Western blot
Protein Immunostaining
Flow cytometry.

Note: SDS PAGE is a method of separating proteins based on size.
ELISA
Chromatin Immunoprecipitation assay
Flow cytometry
SDS - PAGE