Factors affecting sickling in sickle cell anemia are -
Formation of sickle cell depends on HbS concentration since HbS polymerises and grow beyond the RBC membrane ,prject through it. Referance: Exam preparatory manual for UGs -Pathology by Ramdas Nayak Page no:274 Robbins 9/e pg
HbS concentration
HbA
pH
Oxygenation
Haematology