A young boy had a flea bite while working in a wheat grain godown. After 5 days he developed fever and had axillary lymphadenopathy. A smear was sent to the laboratory to perform a specific staining. Which one of the following staining method would help in the identification of the suspected pathogen:
The most likely disease to be caused by the flea bite in a young boy is PLAGUE caused by YERSINIA PESTIS and smear is stained with WAYSON stain. Albe staining:- staining the volutin granules found in Corynebacterium diphtheriae Zeihl Neelson staining:-for Mycobacterium Mc Fadyean staining:-M'Fadyean Stain(Polychrome Methylene blue) is used for capsule staining of Bacillus anthracis.
Albe staining
Zeihl Neelson staining
Mc Fadyean staining
Wayson staining
Systemic Bacteriology (Haemophilus, Yersinia, Spirochaetes, Ricketssia, Chlamydia, Mycoplasma and Miscellaneous Bacteria)