In SDS-PAGE (Sodium Dobecyl Sulfate-polyacrelamide Gel Electrophoresis) proteins are separated on basis of
SDS PAGE electrophoresis is a variant of PAGE. separation of molecules will depend mainly on their molecular size. Hence it is mainly used for molecular weight determination as well as for assessing purity of proteins.Ref: DM Vasudevan - Textbook of Biochemistry, 7th edition, page no: 447
Mass
Charge
Density
Molecular weight
Metabolism of protein and amino acid