Most effective method of sterilization by heat is
Most effective method of sterilization by heat is by using moist heat (steam) above atmospheric pressure Autoclave is a classical example. Principle of autoclave- when water boils under pressure, instead of boiling at 100 degrees, it boils at a higher temperature. Hence the steam generated is at higher temperature.
Hot air
Boiling
Steam
Steam under pressure
General Microbiology (Sterilization and Bacterial Genetics)