Percentage of formalin used for histopathological preservative?
2Ans. (b) 10%Ref: Handbook of Histopathological and Histochemical Techniques 3rd ed. / 40-45* Formalin is generally the preferred fluid for fixation and is widely used. Formalin is often sold as 37-40% aqueous Formaldehyde.* To make a solution of 10% Formalin, nine parts of water are added to one part of 40% (aqueous) Formaldehyde.* Therefore, a 10% solution of Formalin is the equivalent of a 4% solution of Formaldehyde.* Formaldehyde is a gas produced by the oxidation of methyl alcohol, whereas 100% Formalin is a saturated solution of this gas in water.Other Histopathological Preservatives:* Glutaraldehyde: 25%-for electron microscopy (with osmium tetra-chloride)* Acetaldehyde acrolein* Mercuric chloride* Potassium dichromate
5%
10%
20%
40%
Histo Pathology