Which amino acid migrates fastest on chromatography on carboxy methylcellulose medium?
Ai.e. Aspaic acidIn cation exchange chromatography solid stationary matrix has negatively charged groups (i.e. -ve charged carboxy methyl cellulose, 0 - CH2 - COO- is used as cation exchanger). So in the mobile phase, proteins with a net positive charge (i.e. cations) move slowly than those with net negative charge (anion)Q. Hence aspaate (aspaic acid) being a negatively charged amino acid will move fastest in carboxymethyl cellulose or methanol chloroform (cation exchanger) ion exchange chromatography.
Aspaic acid
Valine
Lysine
Glycine