Which of the following is the procedure to separate and detect DNA fragments?
The procedure to separate and detect DNA fragments is termed Southern blotting which is named after Edwin M. Southern. Here, DNA is first isolated and then digested into small fragments with restriction enzymes. Separation of the various segments of DNA is accomplished by incubating the DNA mixture in a gel matrix and then using electrophoresis. The same approach can be used with RNA and is termed Nohern blotting. Finally, a similar procedure can be performed to separate proteins in a procedure called Western blotting. However, in the case of proteins, hybridization is performed using an antibody to the protein of interest rather than a cDNA probe. Note Procedure to separate DNA - Southern Blotting RNA - Nohern Blotting Protein - Western Blotting Ref: Walsh R.A., Jain M.K., Proweller A. (2011). Chapter 6. Principles of Molecular Cardiology. In V. Fuster, R.A. Walsh, R.A. Harrington (Eds), Hurst's The Hea, 13e.
Southern blotting
Nohern blotting
Western blotting
DNA electrophoresis