Which one of the following enzymes is obtained from Thermophillus aquaticus bacterium which is heat stable and used in PCR at high temperature:
Taq polymerase Heat stable Derived from a bacteria; Thermophillus aquaticus or Thermus aquaticus. Lacks proof reading. Thermostable DNA Polymerase enzymes with proof reading function in PCR: Pfu: Pyrococcus furiosus Pwo polymerase: Pyrococcus woesei Tli: Thermococcus litoralis - also known as Vent Polymerase Best- Use Pfu in conjunction with Taq polymerase
DNA polymerase III
Endonuclease
Taq polymerase
DNA gyrase
Techniques in molecular biology