The ability of amino acids/proteins to behave like zwitterions forms the basis for separating them using the following technique
Isoelectric focusing is based on the isoelectric pH (pH at which a molecule carries no net charge). pH gradients created in the gel using polyampholytes. As there is no net charge, molecule stops moving during electrophoresis if they encounter their pI.
Gel filtration chromatography
Ion exchange chromatography
Isoelectric focusing
Mass spectrometry
Classification and metabolism of amino acids