Which of the following enzymes is used in Recombinant DNA research for Homopolymer tailing?
Ans. D. Terminal transferaseSome of the Enzymes Used in Recombinant DNA ResearchEnzymeReactionPrimary UseAlkaline phosphataseDephosphorylates 5' ends of RNA and DNARemoval of 5'-PO4 groups prior to kinase labeling; also used to prevent self-ligationBAL 31 nucleaseDegrades both the 3' and 5' ends of DNAProgressive shortening of DNA moleculesDNA ligaseCatalyses bonds between DNA moleculesJoining of DNA moleculesDNA polymerase ISynthesizes double-stranded DNA from single- stranded DNASynthesis of double-stranded cDNA; nick translation; generation of blunt ends from sticky endsDNase IUnder appropriate conditions, produces single- stranded nicks in DNANick translation; mapping of hypersensitive sites; mapping protein-DNA interactionsExonuclease IIIRemoves nucleotides from 3' ends of DNADNA sequencing; mapping of DNA-protein interactionsExonucleaseRemoves nucleotides from 5' ends of DNADNA sequencingPolynucleotide kinaseTransfers terminal phosphate (position) from ATP to 5'-OH groups of DNA or RNA32P end-labeling of DNA or RNAReverse transcriptaseSynthesizes DNA from RNA templateSynthesis of cDNA from mRNA; RNA (5' end) mapping studiesS1 nucleaseDegrades single-stranded DNARemoval of "hairpin" in synthesis of cDNA; RNA mapping studies (both 5' and 3' ends)Terminal transferaseAdds nucleotides to the 3' ends of DNAHomopolymer tailing
Reverse transcriptase
S1 Transferase
Polynucleotide kinase
Terminal transferase.
Molecular Genetics