6 months old infant with Hb of 6 gm% has anaemic features with peripheral blood showing target cells. Family history of similar complaints is (Positive). Diagnosis can be best done with which of the following –
Presence of :


Anaemia (Hb < 6%),  
Target cells,
Increase reticulocytes suggests the diagnosis of thalassemia.


Thalassemia is a hemoglobin disorder. Hemoglobin disorders are diagnosed by electrophoresis.
Diagnosis of thalassemia is confirmed by serum electrophoresis.
Hb electrophoresis
ELISA
Coomb's Test
Osmotic fragility