Flow cytometry is done to know -
Ans. is 'a' i.e., Rapid cell shrinkage o As there is no inflammation in apoptosis - there is no leukocyte migration.Apoptotic cells or apoptotic bodies (end products) are phagocytosed by macrophages,o Endonuclease causes double stranded breaks of nuclear DNA. The nucleus itself may break up producing two or more fragments.o Activation of caspases is central to the pathogenesis of apoptosis. :-Following are the method to identify the apoptopic cellsStaining of chromatin condension by haematoxylin, Feulgen and acridine orange.Flow cytometry to visualise rapid cell shrinkage.DNA changes in situ technique or by electrophoresis,Annexin V as a marker of apoptotic cell.
Rapid cell shrinkage
Blood flow to brain
Net O2 supply to tissues
Amount of O2 bound to hemoglobin
Hemodynamics