Best fixative for Pap smear:
ANS. AFixatives:* Pap smear: 95% ethanol + 5% ether* Light microscopy: 10% formaldehyde* Electric microscopy: 2% glutaraldehyde* For liquid-based cytology: Methanol* For bone marrow biopsy: Zenker's fluid* For bone marrow aspirate: Helly's fluid* Cell block: Bouin's fluid* Karyotyping fixatives: Carnoy's fixative (3:1 methanol to glacial acetic acid)* Gastrointestinal biopsies: Bouin's fluid.
95% ethanol + 5% ether
10% formaldehyde
80% isopropyl alcohol
90% ether + alcohol
Female Genital Tract