Which of the following result is provided by nohern blot analysis?
Nohern blotting allows researchers to determine the level of production (and, therefore, control of expression) of a paicular mRNA by quantitative determination of the relative amount of that paicular mRNA relative to total mRNA. Nohern blotting is analogous to Southern blotting, a technique that was first described by Edward Southern. DNA fragments are separated on agarose gels by electrophoresis and then trans- ferred to nitrocellulose filters. The filters are then exposed to labeled probes, which hybridize to the DNA fragments. The use of nohern blotting for the quantitation of mRNA abundance has been mainly replaced to a great extent by quantitative reverse-transcription polymerase chain reaction (-PCR) (described below), which is much more sensitive and reliable. Also Know: Western blotting is a biochemical technique, adapted from a similar technique for DNA of Southern blotting, for detection and identification of specific proteins. Ref: Janson L.W., Tischler M.E. (2012). Appendix II. Biochemical Methods. In L.W. Janson, M.E. Tischler (Eds), The Big Picture: Medical Biochemistry.
Detects specific base pairs
Detects DNA molecules
Determines chromosome structure
Detects RNA molecules