All of the following are polar amino acids, EXCEPT:
Nonpolar amino acids (eg, Leu, Ile) migrate the fahest as they spend the greatest propoion of their time in the mobile phase in chromatography. Polar amino acids (eg, Glu, Lys) travel the least distance from the origin as they spend a high propoion of their time in the stationary phase. Ref: Kennelly P.J., Rodwell V.W. (2011). Chapter 3. Amino Acids & Peptides. In D.A. Bender, K.M. Botham, P.A. Weil, P.J. Kennelly, R.K. Murray, V.W. Rodwell (Eds), Harper's Illustrated Biochemistry, 29e.
Isoleucine
Lysine
Arginine
Histidine