Effective polymerase reaction was repeated for 3 cycles on a DNA molecule. Which of the following will be the resulting formation of the copies'?
If polymerase chain reaction is functioning effectively, each cycle will produce the double number of DNA molecules. After one round of PCR replication, a newly synthesized copy of each DNA strand is produced. PCR is defined as a synthetic method of creating many copies of discrete fragments of DNA present in a clinical specimen thus facilitating detection of nucleic acids present initially only in very small quantities. The three steps to amplification by PCR are: Denaturation Primer annealing Extension Indications of PCR are: To detect infectious agent To make prenatal genetic diagnosis To detect genetic allelic polymorphisms To establish precise tissue types for transplants To study evolution using DNA from archaeological samples PCR method Allows DNA detection in a single cell, hair follicle, or spermatozoon Ref: Clinical Laboratory Medicine edited by Kenneth D. McClatchey page 190. Analysis of Genes and Genomes By Richard J. Reece page 156.
Double number of copies
Three times the number of DNA molecule
Four times the number of DNA molecule
8 times the number of DNA molecule