Isoelectric point of a protein is located by which method?
Ans. is 'b' i.e.,Isoelectric cardiac failure Isoelectric focusing is a procedure used to determine the isoelectric point (pI) of a protein.A pH gradient is established by allowing a mixture of low molecular weight organic acids and bases to distribute themselves in an electric field generated across the gel.When a protein mixture is applied, each protein migrates until it reaches the pH that matches its pI.Proteins with different isoelectric points are thus distributed differently throughout the gel.
Isoelectric pointing
Isoelectric cardiac failure
Ion exchange
pH