Chromosomal mutation can be identified by all except:
Chromosomal mutation can't be identified by Agarose gel electrophoresis Agarose Gel electrophoresis Used to separate the DNA by charge or by size. The smaller the fragment, the more rapid the migration. Overall rate of migration and optimal range of size for separation are determined by the chemical nature of the gel and by the degree of its crosslinking. Highly cross-linked gels optimize the separation of small DNA fragments. The dye ethidium bromide forms a brightly fluorescent color as it binds to DNA, and so small amounts of separated DNA fragments can be photographed on gels. Specific DNA fragments can be recognized by probes containing complementary sequences. Usually performed to visualize the amplified DNA after PCR, but may be used as a preparative technique prior to use of other Just a method of visualizing the DNA after separating by size. So it is always used as a pa of any molecular method to visualize the DNA. But alone it cannot be used to detect any mutations.
Single strand polymorphism
Agarose gel electrophoresis
Denaturating Gradient gel electrophoresis
Dideoxynucleotide trail sequencing
General Microbiology (Sterilization and Bacterial Genetics)