Gram-negative bacteria doesn't take gram stain because it is made of
The exact mechanism of gram reaction is not known. The possible reason is: Gram-positive bacteria have a more acidic protoplasm, which accounts for their retaining the basic primary dye more strongly. Dye iodine complex is retained in gram-positive cells by thick peptide glycan mesh and is readily wash through the very thin peptidoglycan layer of gram-negative cells. Ref: Ananthanarayana textbook of Microbiology 9th edition Pgno : 13
Polysaccharide
Lipopolysaccharide
Techoic acid
None of the above
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