Edman's reagent is -
Ans. is 6c' i.e., Phenyl-isocyanateo Determination of amino acid sequence (sequence analysis) is performed by Edman degradation method on automatic mechines, called cyclic sequentors. Edman's reagent is phenyl-isothiocyanate, and forms a covalent bond to N- terminal of amino-acid. This can be identified.o Sanger's reagent (l-fluoro-2,4-dinitrobenzene) can be used in place of Edman's reagent.
2-4 dinitrophenol
1 -fluoro-2, 4-dinitrobenzene
Phenyl-isocyanate
Cynogen bromide
Proteins and Amino Acids