What is involved in formation of d-TMP from d-UMP?
A i.e. N5 - N10 methylene tetrahydrofolated-UMP (deoxy-uridine monophosphate) is conveed to d-TMP (deoxy-thymidine monophosphate) which uses N5, N10 -methylene tetra hydrofolate as a source of methyl group (1 carbon & 2 hydrogen)Q.). This is an unusual reaction in that THF (tetrahydrofolate) contributes not only 1 carbon unit but also 2 hydrogen atoms from pteridine ring , which causes oxidation of THF to DHF. Disorders of folate & vitamin B12 metabolism cause deficiency of TMP.Thymine analog, antitumor drug 5-flurouracil (5Fu) is conveed to 5-F dUMP, which becomes permanently bound to the inactive thymidylate synthaseQ. So 5FU is a suicide inhibitor. Methotrexate (Mtx) and antibacterial trimethoprim inhibits dihydrofolate reductase, an enzyme which reduces DHF to THFQ.
N5, N10-methylene tetra hydrofolate
Form imino folate
N5 formyl folate
Dihydro folate