Which finding on electron microscopy indicates irreversible cell injury?
IRREVERSIBLE INJURYCytoplasmic changes:1. Cells appear glassy and homogeneous mostly because of loss of glycogen molecules. 2. Myelin figures become more prominent 3. Cytoplasm becomes vacuolated and appears "moth-eaten".By electron microscopy1. Discontinuity in plasma and organelle membrane.2. Marked dilatation of mitochondria with an appearance of large amorphous densities.3.Disruption of lysosomes. 4.Intracytoplasmic myelin figures. Nuclear changes: -Karyolysis: Basophilia of chromatin fade. -Pyknosis: nuclear shrinkage and increased basophilia. -Karyorrhexis: Pyknotic nucleus undergo fragmentation. {Reference: Robbins basic pathology 9Edition pg: 5}
Dilatation of endoplasmic reticulum
Dissociation of ribosomes from rough endoplasmic reticulum
Large amorphous densities in the mitochondria
Myelin figures becomes sparse
General pathology