Which of the following is TRUE regarding the conventional cytogenetic analytic method, 'banding'?
During conventional cytogenetic analysis, cells are isolated at metaphase or prometaphase and treated chemically or enzymatically to reveal chromosome "bands". Analysis of the number of chromosomes in the cell and the distribution of bands on individual chromosomes allow the identification of numerical or structural abnormalities. This strategy is useful for characterizing the normal chromosome complement and determining the incidence and types of major chromosome abnormalities. Ref: Schwaz S., Hassold T. (2012). Chapter 62. Chromosome Disorders. In D.L. Longo, A.S. Fauci, D.L. Kasper, S.L. Hauser, J.L. Jameson, J. Loscalzo (Eds), Harrison's Principles of Internal Medicine, 18e.
Method of detecting types of major chromosome abnormalities
Fluorescence filters are used
Y-chromosome is seen in interphase
Trypsin and Giemsa are used