Reducing sugar in urine can be detected by -
Ans. is 'd' i.e., AH of the above Detection of reducing sugarso Reducing property of sugars in alkaline solution is utilized for both qualitative and quantitative determination of sugars. Reagent containing Cu++ ions are most commonly used. These are generally alkaline solution of cupric sulfatei) Benedict's quantitative reagent (CuSO4, Na2CO3, sodium citrate, potassium ferrocyanide, potassium thiocyanide) can detect any reducing sugar.ii) Fehling solution contains CuS04, Ruchelle Salt (sodium potassium tartarate) and strong alkali (NaOH/ KOH). It is not used now.iii) Glucose oxidase method : - This method i s specific for glucose. Peroxidase and oxidase enzymes are used forestimation of glucose. These enzymes are the basis of highly specific test strips used for detction of glucose in urine or blood.
Benedicts test
Fehling solution
Glucose-oxidase test
All of the above
Chemistry of Carbohydrates, Amino sugars, and Mucopolysaccharides