Which of the following will be detected in flow cytometry?
Flow cytometry is an extremely useful investigational tool that allows simultaneous multiparametric analysis of the physical characteristics defining WBC types using the principles of light scattering, excitation, and fluorochrome emission. As such, it can allow for the rapid identification and quantification of populations of WBC in any given patient sample based on the characteristic immunophenotype of the population subset.Also know:Flow cytometry can be a vital investigational tool for the hospitalist. It can be used in the initial workup of a new leukocytosis to identify a malignant versus a benign reactive cause. Flow cytometry also has direct uses in nonmalignant hematologic conditions, such as in the determination of CD4/CD8 count ratios in HIV-associated lymphocytopenia or in the diagnosis of rarer conditions such as paroxysmal nocturnal hemoglobinuria and Langerhans cell histiocytosis. Ref: Leonard B.J., Leber B. (2012). Chapter 174. Disorders of the White Cell. In G.V. Lawry, S.C. McKean, J. Matloff, J.J. Ross, D.D. Dressler, D.J. Brotman, J.S. Ginsberg (Eds), Principles and Practice of Hospital Medicine.
Polycythemia
Thrombocytosis
Leukocytosis
Neutrophilia