All of the following are used in a PCR reaction, EXCEPT
Components of PCR 1. Template DNA 2. Pair of DNA Primers 3. Thermostable DNA polymerase 4. dNTPs 5. Buffer with divalent cation ddNTP (dideoxynucleotide triphosphates) Not used in PCR; Used in sanger's chain-termination method
Buffer
ddNTPs
Oligonucleotide Primer pair
Template DNA
Techniques in molecular biology