In vitro test for virulence of pneumococcus:
Ans: a (Intra peritoneal inoculation in mice) RefAnanthanarayan, ed, p. 220When pneumococci are scanty with negative cultures, isolation can be done by intra peritoneal inoculation in mice for virulent strains. Inoculated mice will die within 1 -3 days and pneumococci may be isolated from peritoneal fluid or heart blood. This will be negative with avirulent strains e.g., type 14.Other tests employed are:1) Gram stain - gram positive, lanceolate diplococcic2) Sputum culture in blood agar3) Blood culture - isolation from blood indicates bad prognosis4) CSF - Gram staining and cultureDemonstration of SSS by precipitation5) Capsular polysaccharide - Quellung reaction, counter immune electrophoresis
Intra peritoneal inoculation in mice
Rat tail inoculation
Mouse foot pad inoculation
Armadillo inoculation
Pneumococci