In SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) proteins are separated on basis of: (PGI June 2009)
Ans: A (Mass) & D (Molecular weight) "The most widely used method for determining purity of of a protein is SDS-PAGEfi-sodium dodecyl sulphate(SDS).ln this polypeptide separates based on their relative molecular mass (Mr)"- Harper 27th/24Methods Used for Protein Separation & AnalysisTechniqueProtein Property ExploitedElectrophoresisQDenaturing gel (SDS-PAGE) QMassQ (Motecular weight)Native gelMass/Charge2 D gelsMolecular weight & plIEF(Isoelctric focussing)plflsoelctric point)Chromatography Methods(r)Ion- ExchangeChargeQGel FiltrationSizeQ or Molecular weightQAffinityBiological activityReversed phaseHydrophobicityHydrophobic InteractionHvdrophobicityChromatofocussingPlBulk MethodsQCentrifugationMassQ/DensityFiltrationSizeQAmmonium sulphateSolubility
Mass
Charge
Density
Molecular weight
Miscellaneous (Structure & Function of Proteins)