The enzyme carbamoyl phosphate synthetase requires:
One molecule of ammonia condenses with CO2 in the presence of two molecules of ATP to form carbamoyl phosphate. The reaction is catalyzed by the mitochondrial enzyme carbamoyl phosphate synthetase-I (CPS-I).
Reference: Vasudevan 7th  ed, pg 203
Mg++
Ca++
K+
None of the above