Structure of proteins can be detected by all the methods, EXCEPT:
High-performance/pressure liquid chromatography (HPLC) is a technique used for enhanced and precise separation of even small amounts of biological and nonbiological molecules. HPLC columns utilize variations of the separation principle behind column chromatography, depending on variable attractive and repulsive forces on the gel material and their interaction with the biological molecules. The analysis of peptides and structure of proteins are done by mass spectrometry. NMR spectroscopy analyzes proteins in aqueous solution. Proteins and protein complexes of >100 kDa can be analyzed by NMR. Ref: Janson L.W., Tischler M.E. (2012). Appendix II. Biochemical Methods. In L.W. Janson, M.E. Tischler (Eds), The Big Picture: Medical Biochemistry.
High pressure liquid chromatography (HPLC)
Mass spectrometry
NMR spectrometry
All of the above