The enzyme used in polymerase chain reaction (PCR) is:
Ans. A. Taq polymerasePolymerase Chain Reaction is a technique employed for DNA amplification. It requires the following apparatus:a. A target DNA.b. Two primers (synthetic oligonucleotides of 17-30 nucleotides length) that are complementary to flanked regions of DNA.c. Four dNTP's.d. A DNA polymerase mainly Taq DNA polymerase (Thermo stable). It has been isolated form Thermus aquaticus. It is heat resistant, so no need to add fresh enzyme in every cycle.
Taq polymerase
RNA polymerase
Ribonuclease
Endonuclease
Molecular Genetics