Tyndallization is a method of sterilization using;
Tyndallization or intermittent sterilization uses moist heat at 100 C for 20 minutes for 3 consecutive days. It is carried out in a Koch and Arnold steam sterilizer. Media which contain any ingredient/component not able to tolerate the temperature of an autoclave are sterilized by tyndallization. Eg. TCBS, XLD, DCA, sugar solutions for fermentation tests and gelatin containing media.
Moist heat above 100 C
Moist heat below 100 C
Dry heat above 100 C
Moist heat at 100 C
General Microbiology (Sterilization and Bacterial Genetics)