What does the term nick translation refer to?
A nick is a discontinuity in a double stranded DNA molecule where there is no phosphodiester bond. Nick is created by DNAse I Nick translation is done by DNA polymerase I Nick sealing is done by DNA ligase Nick translation removes DNA ahead of a nick, synthesizes DNA behind nick and as a net result, moves the nick in the 5' to 3' direction. This propey is used to incorporate radio labeled nucleotides in DNA.
Translation of RNA into polypeptide
Regulation of gene expression
Technique to label DNA
Technique to digest DNA
Techniques in molecular biology