DNA NICK AND LABELLING (insitu) technique is used to see?
DNA NICK AND LABELLING An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION. REF : robbins 10th ed
amount of total DNA in a mitochondria
fraction of nucleic acids
fraction of cells in apoptotic pathway quantified
fraction of cells in meotic division is quantified
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