Two transgenic plants are grown. One plant has a gene encoding a fluorescent pigment incorporated in its genome. The other plant has a fire fly luciferase gene incorporated in its genome. Which of the two plants will glow in the dark?
A i.e. Both plants will glow - Both the plants expressing genes for firefly luciferase and (green/yellow/blue/cyan) fluorescent protein of jelly fish (Aequorea victoria) will glow in darkQ. - Fluorescent probes are derived from green fluorescent protein (GFP) of jelly fish. It is an 11-stranded (3-barrel and light absorbing /emitting center (chromatophore) of protein comprises a modified (oxidized) form of tripeptide - Ser65 - Tyr 66 - G1y68 located within the barrel. Oxidation of tripeptide is catalyzed by GFP itself, with no other protein or cofactor required other than oxygenQ. So it can be cloned into viually any cell, where it can serve as a fluorescent marker for itself or for any protein to which it is fused. When exited by absorption of photon of light GFP emits fluorescent, photon in green region. A related protein (m RFP1) fluoresces red light. Actually luciferase containing transgenic plants emit quite weak light and a prolonged exposure (- 24 hour) pictures show them glowing in dark.
Both plants will glow
Neither will glow
First one will glow
Second one will glow