In the test given below to estimate blood glucose, which of the following is true:
Ans. B. Glucose oxidase is highly specific for beta anomer of GlucoseThe reaction in glucometer isGlucose+H2 O+O2 -GODGluconicacid+H2 O2 2H2 O2 +4aminoantipyrine+PHBS-PODQuinoneiminedye+H2 O* This reaction is specific for beta anomer of glucose* Here the first carbon atom is oxidised* This is an example of enzymatic method (Glucose Oxidase Peroxidase method)
Glucose is converted to glucuronic acid
Glucose oxidase is highly specific for beta anomer of Glucose
The terminal Carbon is oxidised.
This is an example of Reducometric method
Carbohydrates