In a patient with UTI; on smear, no bacteria are found on gram stain with abundant pus cells, to demonstrate organism, which of the following is useful?
Ans. (a) McCoy cell line Complaint of urethritis with no result on gram staining signifies nongonococcal urethritis." MC cause of NGU is Chlamydia trachomatis and urethritis is one of the commonest manifestation of genital chlamydiasis. Diagnosis of genital chlamydiasis (D-K serovars) I. Culture: Scarpe epithelial cells from 1-2 cm deep into endocervix. Dacron, cotton, rayon or calcium alginate on plastic shaft should be used to collect specimen. Inoculum is centrifuged onto the monolayer of cyclohexinide treated McCoy cells. This is incubated at 35-37degC for 48-72 hours. Monolayers can be increased. Examine monolayers by direct IF to see inclusion bodies. This method is 80% sensitive but 100% specific. Remembe:- HeLa cell culture can also be used. Direct cytologic examination (direct fluorescent antibody) and enzyme-linked immunoassay. Nucleic acid detection by PCR and LCR are test of choice to diagnose genital C. trachomatis infections. Serology -Serum antibodies are more common than trachoma because of more antigenicity in genital tract. Remembe:- LJ medium is for Mycobacteria tuberculosis. Thayer Main medium is for Neiserria.
McCoy cell line
Thayer main medium
L. J. medium
Acid fast staining