A variety of basic assays are used to detect autoantibodies. Nephelometry is based on the principle of:
Nephelometry measures the interaction of antibodies and antigens in solution, detecting immune complex formation by monitoring changes in the scattering of an incident light. Ref: Imboden J.B. (2007). Chapter 3. Laboratory Diagnosis. In J.B. Imboden, D.B. Hellmann, J.H. Stone (Eds), CURRENT Rheumatology Diagnosis & Treatment, 2e.
Light attenuated in intensity by scattering
Refraction of light
Reduced transmission of light
Filtration of solutes by kidney