Conversion of lactate to glucose requires all except
Step 3 of Glycolysis i.Fructose-6-phosphate is fuher phosphorylated to fructose1,6-bisphosphate. The enzyme is phosphofructokinase. ii.PFK is an allosteric, inducible, regulatory enzyme. It is an impoant key enzyme of this pathway. This is again an activation process, the energy being derived by hydrolysis of yet another molecule of ATP. This irreversible step is the rate-limiting reaction in glycolysis. The lactate formed in the muscle is transpoed to the liver. In the liver cell lactate dehydrogenase conves lactate to pyruvate (Fig. 9.28). The pyruvate enters the gluconeogenic pathway to form glucoseRef: DM Vasudevan, 7th edition, page no: 109
Pyruvate carboxylase
Phosphofructokinase
PEP carboxykinase
Glucose-6-phosphatase
Metabolism of carbohydrate