

## Dataset Overview
This dataset features the 7 evaluation tasks presented in the AgroNT (A Foundational Large Language Model for Edible Plant
Genomes) paper. The tasks cover single output regression, multi output regression, binary classification, and multi-label classification which
aim to provide a comprehensive plant genomics benchmark. Additionally, we provide results from in silico saturation mutagenesis analysis of sequences
from the cassava genome, assessing the impact of >10 million mutations on gene expression levels and enhancer elements. See the ISM section
below for details regarding the data from this analysis.


| Task Name    | # of Datasets(Species) | Task Type    | Sequence Length (base pair) |
| -------- | ------- | -------- | ------- | 
| Polyadenylation  | 6    | Binary Classification  | 400    |
| Splice Site | 2     | Binary Classification | 398     | 
| LncRNA    | 6    | Binary Classification    | 101-6000  |
| Promoter Strength    | 2    | Single Variable Regression    | 170 |
| Chromatin Accessibility    | 7    | Multi-label Classification   | 1000 |
| Gene Expression    | 6        | Multi-Variable Regression  | 6000 |
| Enhancer Region    | 1        | Binary Classification    | 1000 |


## Dataset Sizes
| Config Name    | # Train Samples | # Validation Samples   | # Test Samples |
| -------- | ------- | -------- | ------- | 
|poly_a.arabidopsis_thaliana|170835|---|30384|
|poly_a.oryza_sativa_indica_group|98139|---|16776|
|poly_a.trifolium_pratense|111138|---|13746|
|poly_a.medicago_truncatula|47277|---|8850|
|poly_a.chlamydomonas_reinhardtii|90378|---|10542|
|poly_a.oryza_sativa_japonica_group|120621|---|20232|
|splicing.arabidopsis_thaliana_donor|2588034|---|377873|
|splicing.arabidopsis_thaliana_acceptor|1704844|---|250084|
|lncrna.m_esculenta|4934|---|360|
|lncrna.z_mays|8423|---|1629|
|lncrna.g_max|11430|---|490|
|lncrna.s_lycopersicum|7274|---|1072|
|lncrna.t_aestivum|11252|---|1810|
|lncrna.s_bicolor|8654|---|734|
|promoter_strength.leaf|58179|6825|7154|
|promoter_strength.protoplast|61051|7162|7595|
|gene_exp.glycine_max|47136|4803|4803|
|gene_exp.oryza_sativa|31244|3702|3702|
|gene_exp.solanum_lycopersicum|27321|3827|3827|
|gene_exp.zea_mays|34493|4483|4483|
|gene_exp.arabidopsis_thaliana|25731|3401|3402|
|chromatin_access.oryza_sativa_MH63_RS2|5120000|14848|14848|
|chromatin_access.setaria_italica|5120000|19968|19968|
|chromatin_access.oryza_sativa_ZS97_RS2|5120000|14848|14848|
|chromatin_access.arabidopis_thaliana|5120000|9984|9984|
|chromatin_access.brachypodium_distachyon|5120000|14848|14848|
|chromatin_access.sorghum_bicolor|5120000|29952|29952|
|chromatin_access.zea_mays|6400000|79872|79872|
|pro_seq.m_esculenta|16852|1229|812|

*** It is important to note that fine-tuning for lncrna was carried out using all datasets in a single training. The reason for this is that the datasets are small and combining
them helped to improve learning.

## In Silico Saturation Mutagensis 
### File structure for: ISM_Tables/Mesculenta_305_v6_PROseq_ISM_LOG2FC.txt.gz
Intergenic enhancer regions based on Lozano et al. 2021 (https://pubmed.ncbi.nlm.nih.gov/34499719/) <br>
Genome version: Manihot esculenta reference genome v6.1 from Phytozome <br>
CHR: Chromosome <br> 
POS: Physical position (bp) <br>
REF: Reference allele <br>
ALT: Alternative allele <br>
LOG2FC: Log fold change in Intergenic enhancer probability (log2(p_mutated_sequence / p_original_sequence)) <br>

### File structure for: ISM_Tables/Mesculenta_v6_GeneExpression_ISM_LOG2FC.txt.gz
Gene expression prediction based on: Wilson et al. 2016 (https://pubmed.ncbi.nlm.nih.gov/28116755/) <br>
Genome version: Manihot esculenta reference genome v6 from Ensembl 56 <br>
CHR: Chromosome <br>
POS: Physical position (bp) <br>
REF: Reference allele <br>
ALT: Alternative allele <br>
GENE: Gene ID <br>
STRAND: Gene strand <br>
TISSUE: Tissue type (Acronyms detailed in Figure 1 of Wilson et al.) <br>
LOG2FC: Gene expression log fold change (log2(gene_exp_mutated_sequence / gene_exp_original_sequence)) <br>
license:cc-by-nc-sa-4.0 DNA Genomics Plants region:us
{"license": "cc-by-nc-sa-4.0", "pretty_name": "Plant Genomic Benchmark", "tags": ["DNA", "Genomics", "Plants"]}
TAGS
#license-cc-by-nc-sa-4.0 #DNA #Genomics #Plants #region-us 

Dataset Overview
----------------


This dataset features the 7 evaluation tasks presented in the AgroNT (A Foundational Large Language Model for Edible Plant
Genomes) paper. The tasks cover single output regression, multi output regression, binary classification, and multi-label classification which
aim to provide a comprehensive plant genomics benchmark. Additionally, we provide results from in silico saturation mutagenesis analysis of sequences
from the cassava genome, assessing the impact of >10 million mutations on gene expression levels and enhancer elements. See the ISM section
below for details regarding the data from this analysis.



Dataset Sizes
-------------



* It is important to note that fine-tuning for lncrna was carried out using all datasets in a single training. The reason for this is that the datasets are small and combining
them helped to improve learning.


In Silico Saturation Mutagensis
-------------------------------


### File structure for: ISM\_Tables/Mesculenta\_305\_v6\_PROseq\_ISM\_LOG2FC.URL


Intergenic enhancer regions based on Lozano et al. 2021 (URL   

Genome version: Manihot esculenta reference genome v6.1 from Phytozome   

CHR: Chromosome   

POS: Physical position (bp)   

REF: Reference allele   

ALT: Alternative allele   

LOG2FC: Log fold change in Intergenic enhancer probability (log2(p\_mutated\_sequence / p\_original\_sequence))   



### File structure for: ISM\_Tables/Mesculenta\_v6\_GeneExpression\_ISM\_LOG2FC.URL


Gene expression prediction based on: Wilson et al. 2016 (URL   

Genome version: Manihot esculenta reference genome v6 from Ensembl 56   

CHR: Chromosome   

POS: Physical position (bp)   

REF: Reference allele   

ALT: Alternative allele   

GENE: Gene ID   

STRAND: Gene strand   

TISSUE: Tissue type (Acronyms detailed in Figure 1 of Wilson et al.)   

LOG2FC: Gene expression log fold change (log2(gene\_exp\_mutated\_sequence / gene\_exp\_original\_sequence))   




### File structure for: ISM\_Tables/Mesculenta\_305\_v6\_PROseq\_ISM\_LOG2FC.URL


Intergenic enhancer regions based on Lozano et al. 2021 (URL   

Genome version: Manihot esculenta reference genome v6.1 from Phytozome   

CHR: Chromosome   

POS: Physical position (bp)   

REF: Reference allele   

ALT: Alternative allele   

LOG2FC: Log fold change in Intergenic enhancer probability (log2(p\_mutated\_sequence / p\_original\_sequence)) ### File structure for: ISM\_Tables/Mesculenta\_v6\_GeneExpression\_ISM\_LOG2FC.URL


Gene expression prediction based on: Wilson et al. 2016 (URL   

Genome version: Manihot esculenta reference genome v6 from Ensembl 56   

CHR: Chromosome   

POS: Physical position (bp)   

REF: Reference allele   

ALT: Alternative allele   

GENE: Gene ID   

STRAND: Gene strand   

TISSUE: Tissue type (Acronyms detailed in Figure 1 of Wilson et al.)   

LOG2FC: Gene expression log fold change (log2(gene\_exp\_mutated\_sequence / gene\_exp\_original\_sequence))
TAGS
#license-cc-by-nc-sa-4.0 #DNA #Genomics #Plants #region-us 
 ### File structure for: ISM\_Tables/Mesculenta\_305\_v6\_PROseq\_ISM\_LOG2FC.URL


Intergenic enhancer regions based on Lozano et al. 2021 (URL   

Genome version: Manihot esculenta reference genome v6.1 from Phytozome   

CHR: Chromosome   

POS: Physical position (bp)   

REF: Reference allele   

ALT: Alternative allele   

LOG2FC: Log fold change in Intergenic enhancer probability (log2(p\_mutated\_sequence / p\_original\_sequence)) ### File structure for: ISM\_Tables/Mesculenta\_v6\_GeneExpression\_ISM\_LOG2FC.URL


Gene expression prediction based on: Wilson et al. 2016 (URL   

Genome version: Manihot esculenta reference genome v6 from Ensembl 56   

CHR: Chromosome   

POS: Physical position (bp)   

REF: Reference allele   

ALT: Alternative allele   

GENE: Gene ID   

STRAND: Gene strand   

TISSUE: Tissue type (Acronyms detailed in Figure 1 of Wilson et al.)   

LOG2FC: Gene expression log fold change (log2(gene\_exp\_mutated\_sequence / gene\_exp\_original\_sequence))