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Molecular evolution of the Paramyxoviridae and Rhabdoviridae multiple-protein-encoding P gene. | Presented here is an analysis of the molecular evolutionary dynamics of the P gene among 76 representative sequences of the Paramyxoviridae and Rhabdoviridae RNA virus families. In a number of Paramyxoviridae taxa; as well as in vesicular stomatitis viruses of the Rhabdoviridae; the P gene encodes multiple proteins from a single genomic RNA sequence. These products include the phosphoprotein (P); as well as the C and V proteins. The complexity of the P gene makes it an intriguing locus to study from an evol | 10666708 | [
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Molecular evolution of the Paramyxoviridae and Rhabdoviridae multiple-protein-encoding P gene. | utionary perspective. Amino acid sequence alignments of the proteins encoded at the P and N loci were used in independent phylogenetic reconstructions of the Paramyxoviridae and Rhabdoviridae families. P-gene-coding capacities were mapped onto the Paramyxoviridae phylogeny; and the most parsimonious path of multiple-coding-capacity evolution was determined. Levels of amino acid variation for Paramyxoviridae and Rhabdoviridae P-gene-encoded products were also analyzed. Proteins encoded in overlapping reading | 10666708 | [
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Molecular evolution of the Paramyxoviridae and Rhabdoviridae multiple-protein-encoding P gene. | frames from the same nucleotides have different levels of amino acid variation. The nucleotide architecture that underlies the amino acid variation was determined in order to evaluate the role of selection in the evolution of the P gene overlapping reading frames. In every case; the evolution of one of the proteins encoded in the overlapping reading frames has been constrained by negative selection while the other has evolved more rapidly. The integrity of the overlapping reading frame that represents a de | 10666708 | [
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Molecular evolution of the Paramyxoviridae and Rhabdoviridae multiple-protein-encoding P gene. | rived state is generally maintained at the expense of the ancestral reading frame encoded by the same nucleotides. The evolution of such multicoding sequences is likely a response by RNA viruses to selective pressure to maximize genomic information content while maintaining small genome size. The ability to evolve such a complex genomic strategy is intimately related to the dynamics of the viral quasispecies; which allow enhanced exploration of the adaptive landscape. | 10666708 | [
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Mitochondrial genomes of Galathealinum, Helobdella, and Platynereis: sequence and gene arrangement comparisons indicate that Pogonophora is not a phylum and Annelida and Arthropoda are not sister taxa. | We report a contiguous region of more than half (> 7;500 nt) of the mitochondrial genomes for Platynereis dumerii (Annelida: Polychaeta); Helobdella robusta (Annelida: Hirudinida); and Galathealinum brachiosum (Pogonophora: Perviata). The relative arrangements of all 22 genes identified for Helobdella and Galathealinum are identical to one another and to their arrangements in the mtDNA of the previously studied oligochaete annelid Lumbricus. In contrast; Platynereis differs from these taxa in the positions | 10666709 | [
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Mitochondrial genomes of Galathealinum, Helobdella, and Platynereis: sequence and gene arrangement comparisons indicate that Pogonophora is not a phylum and Annelida and Arthropoda are not sister taxa. | of several tRNA genes and in having two additional tRNA genes (trnC and trnM) and a large noncoding sequence in this region. Comparisons of relative gene arrangements and of the nucleotide and inferred amino acid sequences among these and other published taxa provide strong support for an annelid-mollusk clade that excludes arthropods; and for the inclusion of pogonophorans within Annelida; rather than giving them separate phylum status. Gene arrangement comparisons include the first use of a recently descr | 10666709 | [
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Mitochondrial genomes of Galathealinum, Helobdella, and Platynereis: sequence and gene arrangement comparisons indicate that Pogonophora is not a phylum and Annelida and Arthropoda are not sister taxa. | ibed method on previously unpublished data. Although a variety of alternative initiation codons are typically used by mitochondrial protein-encoding genes; ATG appears to be the initiator for all but one reported here. The large noncoding region (1;091 nt) identified in Platynereis has no significant sequence similarity to the noncoding region of Lumbricus; although each contains runs of TA dinucleotides and of homopolymers; which could potentially serve as signaling elements. There is strong bias for synon | 10666709 | [
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Mitochondrial genomes of Galathealinum, Helobdella, and Platynereis: sequence and gene arrangement comparisons indicate that Pogonophora is not a phylum and Annelida and Arthropoda are not sister taxa. | ymous codon usage in Helobdella and especially in Galathealinum. In this latter taxon; 5 codons are completely unused; 13 are used three or fewer times; and G appears at third codon positions in only 26 of the 2;236 codons. Nucleotide composition bias appears to influence amino acid composition of the proteins. | 10666709 | [
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Mitochondrial genomic rearrangements in songbirds. | The organization of the mitochondrial genome is generally very conserved among vertebrates. Because of this; examination of the rare rearrangements which do occur has been suggested as offering a powerful alternative to phylogenetic analyses of mitochondrial DNA sequences. Here; we report on an avian mitochondrial rearrangement in a group of oscine passerines (warblers of the genus Phylloscopus). This rearrangement is identical to the mitochondrial organization recently identified in representatives of four | 10666710 | [
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Mitochondrial genomic rearrangements in songbirds. | orders of birds; including subsoscine Passeriformes. The rearrangement involves the movement of three genes (tRNA(Pro); NADH6; and tRNA(Glu)) from their normal position in birds between tRNA(Thr) and the control region (CR); to a new location between the CR and a novel; supposedly noncoding (NC); region. Our results suggest that this derived arrangement cannot be used to distinguish between suboscine and oscine passerines; as it has multiple origins both within Passeriformes and within birds as a whole. We | 10666710 | [
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Mitochondrial genomic rearrangements in songbirds. | found short stretches of DNA with high degrees of similarity between the CR and each NC region; respectively; all of which could be located in the same area of the CR. This suggests that the CR and the NC region are homologous and that the mechanism behind this mitochondrial rearrangement is a tandem duplication followed by multiple deletions. However; the similarities between the control and NC regions of each species were less pronounced than those between the control or NC regions from the different spe | 10666710 | [
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Mitochondrial genomic rearrangements in songbirds. | cies; supporting the hypothesis of a single basal rearrangement in the Phylloscopus warblers. | 10666710 | [
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Rh gene evolution in primates: study of intron sequences. | By amplification and sequencing of RH gene intron 4 of various primates we demonstrate that an Alu-Sx-like element has been inserted in the RH gene of the common ancestor of humans; apes; Old World monkeys; and New World monkeys. The study of mouse and lemur intron 4 sequences allowed us to precisely define the insertion point of the Alu-Sx element in intron 4 of the RH gene ancestor common to Anthropoidea. Like humans; chimpanzees and gorillas possess two types of RH intron 4; characterized by the presence | 10666712 | [
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Rh gene evolution in primates: study of intron sequences. | (human RHCE and ape RHCE-like genes) or absence (human RHD and ape RHD-like genes) of the Alu-Sx element. This led us to conclude that in the RH common ancestor of humans; chimpanzees; and gorillas; a duplication of the common ancestor gene gave rise to two genes; one differing from the other by a 654-bp deletion encompassing an Alu-Sx element. Moreover; most of chimpanzees and some gorillas posses two types of RHD-like intron 4. The introns 4 of type 1 have a length similar to that of human RHD intron 4; | 10666712 | [
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Rh gene evolution in primates: study of intron sequences. | whereas introns 4 of type 2 display an insertion of 12 bp. The latest insertion was not found in the human genome (72 individuals tested). The study of RH intron 3 length polymorphism confirmed that; like humans; chimpanzees and gorillas possess two types of intron 3; with the RHD-type intron 3 being 289 bases shorter than the RHCE intron 3. By amplification and sequencing of regions encompassing introns 3 and 4; we demonstrated that chimpanzee and gorilla RH-like genes displayed associations of introns 3 a | 10666712 | [
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Rh gene evolution in primates: study of intron sequences. | nd 4 distinct to those found in man. Altogether; the results demonstrate that; as in humans; chimpanzee and gorilla RH genes experienced intergenic exchanges. | 10666712 | [
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A phylogenetic analysis of the lipocalin protein family. | The lipocalins are a family of extracellular proteins that bind and transport small hydrophobic molecules. They are found in eubacteria and a great variety of eukaryotic cells; in which they play diverse physiological roles. We report here the detection of two new eukaryotic lipocalins and a phylogenetic analysis of 113 lipocalin family members performed with maximum-likelihood and parsimony methods on their amino acid sequences. Lipocalins segregate into 13 monophyletic clades; some of which are grouped in | 10666711 | [
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A phylogenetic analysis of the lipocalin protein family. | well-supported superclades. An examination of the G + C content of the bacterial lipocalin genes and the detection of four new conceptual lipocalins in other eubacterial species argue against a recent horizontal transfer as the origin of prokaryotic lipocalins. Therefore; we rooted our lipocalin tree using the clade containing the prokaryotic lipocalins. The topology of the rooted lipocalin tree is in general agreement with the currently accepted view of the organismal phylogeny of arthropods and chordates | 10666711 | [
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A phylogenetic analysis of the lipocalin protein family. | . The rooted tree allows us to assign polarity to character changes and suggests a plausible scenario for the evolution of important lipocalin properties. More recently evolved lipocalins tend to (1) show greater rates of amino acid substitutions; (2) have more flexible protein structures; (3) bind smaller hydrophobic ligands; and (4) increase the efficiency of their ligand-binding contacts. Finally; we found that the family of fatty-acid-binding proteins originated from the more derived lipocalins and ther | 10666711 | [
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A phylogenetic analysis of the lipocalin protein family. | efore cannot be considered a sister group of the lipocalin family. | 10666711 | [
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Pattern of morphological diversification in the Leptocarabus ground beetles (Coleoptera: Carabidae) as deduced from mitochondrial ND5 gene and nuclear 28S rDNA sequences. | Most of the mitochondrial NADH dehydrogenase subunit 5 (ND5) gene and a part of nuclear 28S ribosomal RNA gene were sequenced for 14 species of ground beetles belonging to the genus Leptocarabus. In both the ND5 and the 28S rDNA phylogenetic trees of Leptocarabus; three major lineages were recognized: (1) L. marcilhaci/L. yokoael/Leptocarabus sp. from China; (2) L. koreanus/L. truncaticollis/L. seishinensis/L. semiopacus/L. canaliculatus/L. kurilensis from the northern Eurasian continent including Korea and | 10666713 | [
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Pattern of morphological diversification in the Leptocarabus ground beetles (Coleoptera: Carabidae) as deduced from mitochondrial ND5 gene and nuclear 28S rDNA sequences. | Hokkaido; Japan; and (3) all of the Japanese species except L. kurilensis. Clustering of the species in the trees is largely linked to their geographic distribution and does not correlate with morphological characters. The species belonging to different species groups are clustered in the same lineages; and those in the same species group are scattered among the different lineages. One of the possible interpretations of the present results would be that morphological transformations independently took plac | 10666713 | [
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Pattern of morphological diversification in the Leptocarabus ground beetles (Coleoptera: Carabidae) as deduced from mitochondrial ND5 gene and nuclear 28S rDNA sequences. | e in the different lineages; sometimes with accompanying parallel morphological evolution; resulting in the occurrence of the morphological species belonging to the same species group (= type) in the different lineages. | 10666713 | [
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The evolution of vertebrate antigen receptors: a phylogenetic approach. | Classical T cells; those with alpha beta T-cell receptors (TCRs); are an important component of the dominant paradigm for self-nonself immune recognition in vertebrates. alpha beta T cells recognize foreign peptide antigens when they are bound to MHC molecules on the surfaces of antigen-presenting cells. gamma delta T cells bear a similar receptor; and it is often assumed that these T cells also require specialized antigen-presenting molecules for immune recognition; which we term "indirect antigen recognit | 10666714 | [
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The evolution of vertebrate antigen receptors: a phylogenetic approach. | ion." B-cell receptors; or immunoglobulins; bind directly to antigens without the help of a specialized antigen-presenting molecule. Phylogenetically; it has been assumed that T-cell receptors and the genes that encode them are a monophyletic group; and that "indirect" antigen recognition evolved before the split into two types of TCR. Recently; however; it has been proposed that gamma delta-TCRs bind directly to antigens; as do immunoglobulins (Ig's). This calls into question the null hypothesis that indir | 10666714 | [
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The evolution of vertebrate antigen receptors: a phylogenetic approach. | ect antigen recognition is a common characteristic of TCRs and; by extension; the hypothesis that all TCR gene sequences form a monophyletic group. To determine whether alternative explanations for antigen recognition and other historical relationships among TCR genes might be possible; we performed phylogenetic analyses on amino acid sequences of the constant and variable regions which encode the basic subunits of TCR and Ig molecules. We used both maximum-parsimony and genetic distance-based methods and c | 10666714 | [
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The evolution of vertebrate antigen receptors: a phylogenetic approach. | ould find no strong support for the hypothesis of TCR monophyly. Analyses of the constant region suggest that TCR gamma or delta sequences are the most ancient; implying that the ancestral immune cell was like a modern gamma delta T cell. From this gamma delta-like ancestor arose alpha beta T cells and B cells; implying that indirect antigen recognition is indeed a derived property of alpha beta-TCRs. Analyses of the variable regions are complicated by strong selection on antigen-binding sequences; but impl | 10666714 | [
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The evolution of vertebrate antigen receptors: a phylogenetic approach. | y that direct antigen binding is the ancestral condition. | 10666714 | [
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A comparison of estimators of the population recombination rate. | Three new estimators of the population recombination rate C = 4Nr are introduced. These estimators summarize the data using the number of distinct haplotypes and the estimated minimum number of recombination events; then calculate the value of C that maximizes the likelihood of obtaining the summarized data. They are compared with a number of previously proposed estimators of the recombination rate. One of the newly proposed estimators is generally better than the others for the parameter values considered | 10666715 | [
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A comparison of estimators of the population recombination rate. | here; while the three programs that calculate maximum-likelihood estimates give conflicting results. | 10666715 | [
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] |
Correlations among amino acid sites in bHLH protein domains: an information theoretic analysis. | An information theoretic approach is used to examine the magnitude and origin of associations among amino acid sites in the basic helix-loop-helix (bHLH) family of transcription factors. Entropy and mutual information values are used to summarize the variability and covariability of amino acids comprising the bHLH domain for 242 sequences. When these quantitative measures are integrated with crystal structure data and summarized using helical wheels; they provide important insights into the evolution of thr | 10666716 | [
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Correlations among amino acid sites in bHLH protein domains: an information theoretic analysis. | ee-dimensional structure in these proteins. We show that amino acid sites in the bHLH domain known to pack against each other have very low entropy values; indicating little residue diversity at these contact sites. Noncontact sites; on the other hand; exhibit significantly larger entropy values; as well as statistically significant levels of mutual information or association among sites. High levels of mutual information indicate significant amounts of intercorrelation among amino acid residues at these va | 10666716 | [
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Correlations among amino acid sites in bHLH protein domains: an information theoretic analysis. | rious sites. Using computer simulations based on a parametric bootstrap procedure; we are able to partition the observed covariation among various amino acid sites into that arising from phylogenetic (common ancestry) and stochastic causes and those resulting from structural and functional constraints. These results show that a significant amount of the observed covariation among amino acid sites is due to structural/functional constraints; over and above the covariation arising from phylogenetic constraint | 10666716 | [
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Polymorphism and divergence in the beta-globin replication origin initiation region. | DNA sequence polymorphism and divergence was examined in the vicinity of the human beta-globin gene cluster origin of replication initiation region (IR); a 1.3-kb genomic region located immediately 5' of the adult-expressed beta-globin gene. DNA sequence variation in the replication origin IR and 5 kb of flanking DNA was surveyed in samples drawn from two populations; one African (from the Gambia; West Africa) and the other European (from Oxford; England). In these samples; levels of nucleotide and length p | 10666717 | [
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Polymorphism and divergence in the beta-globin replication origin initiation region. | olymorphism in the IR were found to be more than two times as high as adjacent non-IR-associated regions (estimates of per-nucleotide heterozygosity were 0.30% and 0.12%; respectively). Most polymorphic positions identified in the origin IR fall within or just adjacent to a 52-bp alternating purine-pyrimidine ((RY)n) sequence repeat. Within- and between-populations divergence is highest in this portion of the IR; and interspecific divergence in the same region; determined by comparison with an orthologous s | 10666717 | [
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Polymorphism and divergence in the beta-globin replication origin initiation region. | equence from the chimpanzee; is also pronounced. Higher levels of diversity in this subregion are not; however; primarily attributable to slippage-mediated repeat unit changes; as nucleotide substitution contributes disproportionately to allelic heterogeneity. An estimate of helical stability in the sequenced region suggests that the hypervariable (RY)n constitutes the major DNA unwinding element (DUE) of the replication origin IR; the location at which the DNA duplex first unwinds and new strand synthesis | 10666717 | [
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Polymorphism and divergence in the beta-globin replication origin initiation region. | begins. These findings suggest that the beta-globin IR experiences a higher underlying rate of neutral mutation than do adjacent genomic regions and that enzyme fidelity associated with the initiation of DNA replication at this origin may be compromised. The significance of these findings for our understanding of eukaryotic replication origin biology is discussed. | 10666717 | [
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Did the mitochondrial processing peptidase evolve from a eubacterial regulator of gene expression? | nan | 10666719 | [
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Weighted neighbor joining: a likelihood-based approach to distance-based phylogeny reconstruction. | We introduce a distance-based phylogeny reconstruction method called "weighted neighbor joining;" or "Weighbor" for short. As in neighbor joining; two taxa are joined in each iteration; however; the Weighbor criterion for choosing a pair of taxa to join takes into account that errors in distance estimates are exponentially larger for longer distances. The criterion embodies a likelihood function on the distances; which are modeled as correlated Gaussian random variables with different means and variances; c | 10666718 | [
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Weighted neighbor joining: a likelihood-based approach to distance-based phylogeny reconstruction. | omputed under a probabilistic model for sequence evolution. The Weighbor criterion consists of two terms; an additivity term and a positivity term; that quantify the implications of joining the pair. The first term evaluates deviations from additivity of the implied external branches; while the second term evaluates confidence that the implied internal branch has a positive branch length. Compared with maximum-likelihood phylogeny reconstruction; Weighbor is much faster; while building trees that are qualit | 10666718 | [
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[De novo cancer and adenoma-carcinoma sequence of the colorectum--clinicopathological differences between de novo carcinoma and carcinoma with the sequence]. | The adenoma-carcinoma sequence ("The great majority of colorectal carcinomas arises from adenomas") proposed by Morson et al (1972) has been generally accepted world wide. Considering the sequence from the viewpoint of human carcinogenesis in general; however; a few contradictions and mysterious phenomena are found in the development and growth process of the colorectal carcinomas. It is evident that the histogenesis of the adenoma-carcinoma sequence including such contradictions is logically false. Consequ | 10666721 | [
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[De novo cancer and adenoma-carcinoma sequence of the colorectum--clinicopathological differences between de novo carcinoma and carcinoma with the sequence]. | ently; we should fundamentally reconsider the histogenesis of colorectal carcinoma. The premise for the induction of the histogenesis of colorectal carcinoma is that carcinomas are much more objectively differentiated from adenomas with severe atypia; and that hyperplastic glands with slight atypia are differentiated from adenomas with slight atypia. In order to objectify the histological interpretation of atypical grades; conversion of complicated histological designs into real numbers is required. Two dis | 10666721 | [
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[De novo cancer and adenoma-carcinoma sequence of the colorectum--clinicopathological differences between de novo carcinoma and carcinoma with the sequence]. | criminant functions with two variables (N/C ratio and density of tubuli in a unit area) for differentiating carcinoma from adenoma have been determined using computed image processing. Based on the discriminant functions for differentiating objectively between carcinoma; adenoma; and hyperplasia; the histogenesis of colorectal carcinomas has been deduced as follows: 70-80% of colorectal carcinomas arise from the colorectal mucosa (de novo carcinoma) and 20-30% arise from adenoma. Observing the various clini | 10666721 | [
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[De novo cancer and adenoma-carcinoma sequence of the colorectum--clinicopathological differences between de novo carcinoma and carcinoma with the sequence]. | copathological phenomena in colorectal adenomas and carcinomas from the viewpoint of histogenesis; the contradictions do not enter the logically constructed model. Furthermore; the biological behavior of de novo carcinoma is clinicopathologically different from that of carcinoma with the sequence. | 10666721 | [
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[Endoscopic mucosal resection]. | Endoscopic mucosal resection (EMR) is a widely used and important technique for the treatment of colorectal neoplasms. The choice must be made between EMR and surgical resection in treating submucosal invading carcinoma. Our study suggests that EMR may be adapted to the treatment of sm1a and sm1b carcinoma without invading vessels. Endoscopic findings suggestive of submucosal invasion are depressed-type tumor; large size; hardness; ulceration on the top of the lesion; etc. We have conducted studies on the s | 10666722 | [
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[Endoscopic mucosal resection]. | urface structure of colorectal neoplasms (pit pattern) and showed that pit patterns are useful in the qualitative and quantitative diagnosis of lesions; when employing magnifying endoscopy. The VN-type pit pattern indicates that the lesion is most likely sm-massive cancer. This type of cancer should not be treated by EMR. Although minute colorectal cancer can be treated by EMR. We believe that the importance of endoscopic diagnosis and treatment will increase. | 10666722 | [
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[Usefulness of endoscopic polypectomy in early colorectal cancer]. | This study was carried out to clarify the usefulness of endoscopic polypectomy in early colorectal cancer. Six hundred and sixty-four patients with polypectomized early colorectal cancer were compared with 89 who underwent surgical resection. Among the polypectomized patients; 361 had pedunculated (Ip)-type; 255 semi-pedunculated (Isp)-type; and 48 sessile (Is)-type cancer. The early colorectal cancer was submucosal-invasive cancer (sm cancer) in 16.1% of Ip; 19.2% of I sp; and 22.9% of Is patients. Among t | 10666723 | [
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[Usefulness of endoscopic polypectomy in early colorectal cancer]. | he surgically resected patients; sm cancer was present in 41.2% of Ip. 62.1% of I sp. and 58.1% of Is patient in. The percentage of sm cancer and the tumor size surgically resected patients were greater than in polypectomized patients. Local recurrence and distant metastases were investigated after endoscopic polypectomy during follow-up. Two cases (0.4%) of local recurrence were detected after polypectomy among 555 patients with mucosal cancer. Twenty-three (19.8%) of 116 patients with sm cancer underwent | 10666723 | [
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[Usefulness of endoscopic polypectomy in early colorectal cancer]. | surgical resection after polypectomy due to cancer invasion into the vessels and/or extension to the resected margin recognized in polypectomized specimens. Only one case (0.9%) of local recurrence and no distant metastases were detected after polypectomy in 116 patients with sm cancer. Endoscopic polypectomy is very useful as a minimally invasive surgical technique in the protruding type of early colorectal cancer. | 10666723 | [
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[Transanal endoscopic microsurgery (TEM)]. | Minimally invasive surgery is mandatory for rectal tumors to reduce surgical complications and to improve the quality of life. The conventional transanal procedure is one from of minimally invasive surgery for rectal tumors; but it is still often difficult to reach the middle and upper thirds of the rectum due to anatomical characteristics. Transanal endoscopic microsurgery (TEM) is a technique that allows radical resection of rectal tumors in the distal as well as proximal third of the rectum. Performing r | 10666724 | [
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[Transanal endoscopic microsurgery (TEM)]. | adical surgery using this technique requires preoperative estimation of tumor extension both histologically and by X-ray images. It should allow provide safe and radical excision of rectal tumors. In our department; patients with rectal tumors are evaluated for risk factors using barium enema; endoscopic ultrasound; punch biopsy; etc. Fifty patients (rectal cancer; 34: cartinoid; 4: adenoma; 12) have undergone radical TEM. The operation was converted to open surgery in 2 cases because of rectal perforation. | 10666724 | [
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[Transanal endoscopic microsurgery (TEM)]. | Radical open surgery was performed after TEM in 1 patient since cancer invasion was revealed by postoperatively upon histological examination. We conclude that TEM is a useful technique for radical resection of rectal tumors and is associated with a low rate of postoperative complications and a high surgical success rate. | 10666724 | [
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[Minimally invasive transanal surgery]. | A new anal retractor connected to an Octopus retractor holder and automatic suture clamp were used for local excision of rectal tumors to obtain easy access to proximal tumors with an adequate surgical field and to avoid complications. Shortening techniques and invagination techniques were adopted to pull down proximal tumors. This surgical procedure resulted in shorter operating time; less bleeding; earlier oral intake; shorter hospital stay; and fewer complications. | 10666725 | [
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[Laparoscopic bowel resection for early colon cancer]. | Following the successful introduction of laparoscopic cholecystectomy; many reports confirming the feasibility of using laparoscopy for bowel resection and predicting that it would be advantageous in terms of its minimal invasiveness have been published. In the context of cancer treatment; however; the feasibility of lymphadenectomy; the risk of recurrence; and survival have emerged as major concerns. Even though mucosal cancer (Tis) can be treated by endoscopic resection (ER); when this is not possible ope | 10666726 | [
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[Laparoscopic bowel resection for early colon cancer]. | n surgery (OS) must be performed. In patients with T1 cancer; tumors showing slight submucosal layer invasion (sm 1) can be treated in the same way as Tis (in cancer) cancers. But 5% to 10% of patients with T1 cancer have massive submucosal layer invasion (sm 2-3) with paracolic lymph node metastasis. At least partial bowel resection with paracolic lymphadenectomy is considered necessary for T1 (sm 2-3) cancers in principle. In summary; laparoscopic local excision of Tis cancers that are endoscopically unre | 10666726 | [
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[Laparoscopic bowel resection for early colon cancer]. | sectable and laparoscopically assisted partial resection with paracolic lymphadenectomy for T1 cancers have become accepted because local excision and partial resection with paracolic lymphnedectomy are fairly simple to perform laparoscopically. Therefore as a strategy for the treatment of early colorectal cancer (CRC); minimally invasive laparoscopic bowel resection (LBR) has been positioned between endoscopic resection (ER) and open surgery (OS). While the difficulty of performing radical lymphadenectomy | 10666726 | [
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[Laparoscopic bowel resection for early colon cancer]. | is considered one of the greatest obstacles to the introduction of laparoscopic bowel resection (LBR) for the treatment of advanced colorectal cancer (CRC); early colon cancer is a good indication for laparoscopic bowel resection. | 10666726 | [
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] |
[Laparoscopic surgery for early rectal carcinoma]. | The indications; techniques; and results of laparoscopic surgery for early rectal carcinoma are described in detail. Laparoscopic surgery is indicated when a mucosal tumor is too large to perform endoscopic or transanal resection or the tumor invades the submucosal layer. When the tumor is located in the Rs or Ra region; surgery can be completed laparoscopically. After dissection of the mesenterium and lymphadenectomy are performed; the anal side of the rectum is divided using EndoGIA II. When the tumor is | 10666727 | [
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[Laparoscopic surgery for early rectal carcinoma]. | located in the Rb portion; it is impossible to resect the rectum intracorporeally. In this situation; aperi anal maneuver is essential. The rectal mucosa is circumferentially incised just above the dentate line and the internal anal sphincter is dissected. Dissection is advanced to the intersphincteric space. Dissection between the rectum and the levator ani muscle is completed; and the rectum is pulled through the anus. After the oral side of the rectum is divided; a J-pouch is mase and J-pouch-anal anasto | 10666727 | [
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[Laparoscopic surgery for early rectal carcinoma]. | mosis is performed. Forty-seven patients with rectal carcinoma were operated upon laparoscopically. Postoperative recovery was better than that after open surgery. Serious intraoperative or postoperative complications have not been encountered in this series. In conclusion; laparoscopic surgery is thought to be the procedure of first choice for early rectal carcinoma. | 10666727 | [
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[First successful bilateral living-donor lobar lung transplantation in Japan]. | No successful lung transplantation has been reported in Japan until recently when we performed the first successful bilateral living-donor lobar lung transplantation. A 24-year-old woman with primary ciliary dyskinesia began experiencing severe respiratory insufficiency and required mechanical ventilation. On October 28; 1998; she underwent bilateral living-donor lobar transplantation; receiving her sister's lower right lobe and her mother's left lower lobe under cardiopulmonary bypass. The patient was disc | 10666728 | [
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[First successful bilateral living-donor lobar lung transplantation in Japan]. | harged from the hospital 61 days after transplantation. Six months postoperatively; she has returned to normal life and is able to carry out daily activities. She is in good physical condition with a vital capacity of 1.77 L. | 10666728 | [
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[Treatment of an iliac artery pseudoaneurysm managed with a stent graft]. | Recent developments have extended the indications for endovascular intervention to include endovascular lesions; such as aneurysms and sclerotic arterioocclusive disease. Although conventional treatment of pseudoaneurysms is mainly surgical intervention; the development of stent grafts has made this treatment less invasive than previously. We placed a stent graft in a pseudoaneurysm of the iliac artery with good results; as described in this report. Treatment of a pseudoaneurysm with a stent graft avoids th | 10666729 | [
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[Treatment of an iliac artery pseudoaneurysm managed with a stent graft]. | e risk associated with general anesthesia and reduces surgical invasiveness; similar to laparotomy. We expect that this therapeutic mode will be developed further in the future. | 10666729 | [
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[Experience of orthotopic liver transplantation from non-heart-beating donors at the University of Pittsburgh Medical Center]. | Eight cases of orthotopic liver transplantation (OLT) from non-heart-beating donors (NHBDs) were experienced at the University of Pittsburgh Medical Center. Four cases were from donors whose organs were procured following cardiopulmonary resuscitation (uncontrolled NHBD); and the remaining four hepatic allografts were recovered after sustained cardiopulmonary arrest following extubation in an operating room (controlled NHBD). After OLT from uncontrolled NHBDS; two allografts failed due to preservation injur | 10666730 | [
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[Experience of orthotopic liver transplantation from non-heart-beating donors at the University of Pittsburgh Medical Center]. | y and hepatic arterial thrombosis; and one showed poor allograft function. In contrast; all four grafts from controlled NHBDs survived and functioned well worked postoperatively. Hepatic allografts from controlled NHBDs is considered to be useful in OLT; especially in Japan where there is a serious brain-dead donor shortage. | 10666730 | [
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[Electrophysiological mapping of the trigeminal nerve root during microvascular decompression for trigeminal neuralgia]. | A method for intraoperative electrophysiological mapping of the intracranial root of the trigeminal nerve was studied in five patients with trigeminal neuralgia. During surgery; the trigeminal nerve root was stimulated centrally with a bipolar electrode; and antidromic responses were recorded peripherally from three branches of the trigeminal nerve in the face. In all patients; the fibers of the individual subdivisions of the trigeminal nerve root were successfully localized based on the peripheral sites of | 10666732 | [
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[Electrophysiological mapping of the trigeminal nerve root during microvascular decompression for trigeminal neuralgia]. | antidromic response. This neural mapping was used during microvascular decompression in four patients and during a rhizotomy procedure in one patient. As a result of mapping; the fibers of the trigeminal division subserving the pain were clearly confirmed to be compressed by the artery in all four patients who were undergoing microvascular decompression. Likewise; the antidromic responses precisely identified the first division of the trigeminal nerve; which should be preserved to avoid postoperative corne | 10666732 | [
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[Electrophysiological mapping of the trigeminal nerve root during microvascular decompression for trigeminal neuralgia]. | al ulcers in patients undergoing rhizotomy. Based on these findings; it was concluded that this technique enables surgeons to precisely identify which fibers of the trigeminal nerve root should be decompressed or divided during surgery for trigeminal neuralgia. | 10666732 | [
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[Epilepsy surgery for focal cortical dysplasia and dysembryoplastic neuroepithelial tumor]. | We studied six patients with focal cortical dysplasia (CD) and four patients with dysembryoplastic neuroepithelial tumor (DNT) who had surgical resection for medically intractable epilepsy. In all CD patients; ictal single photon emission computed tomography (SPECT) using 99mTc-ECD revealed hyperperfusion in the regions where magnetic resonance (MR) imaging showed CD abnormalities. Interictal epileptiform activity and ictal seizure onset on electrocorticography using subdural strip or grid electrodes were d | 10666733 | [
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[Epilepsy surgery for focal cortical dysplasia and dysembryoplastic neuroepithelial tumor]. | emonstrated in the CD itself. In contrast; in all DNT patients; interictal SPECT disclosed hypoperfusion in the area of the lesions. Ictal SPECT in one DNT patient disclosed hyperperfusion in the superior area of the region where MR imaging showed cystic abnormalities. Interictal spiking in all DNT patients and ictal seizure onset in two DNT patients were demonstrated not in the lesions themselves but in the distinct zone from the region of the tumor-involved brain. All CD patients who underwent lesionectom | 10666733 | [
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[Epilepsy surgery for focal cortical dysplasia and dysembryoplastic neuroepithelial tumor]. | y became seizure-free with a mean follow-up period of 33.5 months. All DNT patients who underwent lesionectomy and resection of the epileptogenic cortex became seizure-free or had their seizure significantly reduced a mean follow-up period of 41.5 months. We conclude that CDs have intrinsic epileptogenicity; while DNTs have epileptogenicity not intrinsically but in encompassed cortical surface areas. | 10666733 | [
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[An ultrasonic study of the relationship between extracranial carotid atherosclerosis and intracerebral hemorrhage]. | We have evaluated the relationship between carotid atherosclerotic change and intracerebral hemorrhage patients. Forty-eight patients with intracerebral hemorrhage treated at our institution were included in this study. Their ages ranged from 38 to 86 years old (average: 61.5 years). There were 28 cases of putaminal hemorrhage and 20 cases of thalamic hemorrhage. Evacuation of hematoma or ventricle drainage was performed in 18 cases within 3 days after the onset of symptoms. The outcome in these cases was t | 10666734 | [
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[An ultrasonic study of the relationship between extracranial carotid atherosclerosis and intracerebral hemorrhage]. | hat 40 patients survived and 8 patients died. Carotid atherosclerosis was evaluated by B mode-ultrasonography. The severity of carotid atherosclerosis was assessed by using two indicators; incidence of carotid atherosclerosis and maximum percentage diameter of the stenosis. Carotid atherosclerosis on B mode-ultrasonography was detected more frequently in patients with thalamic hemorrhage (84.2%) than in those with putaminal hemorrhage (51.7%). Maximum percentage stenosis of thalamic hemorrhage (17.2 +/- 15. | 10666734 | [
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[An ultrasonic study of the relationship between extracranial carotid atherosclerosis and intracerebral hemorrhage]. | 2%) was higher than maximum percentage stenosis of putaminal hemorrhage (6.4 +/- 9.7%). In conclusion; carotid atherosclerosis was an effective indicator of not only ischemical cerebrovascular disease but also of intracerebral hemorrhage. | 10666734 | [
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[Spinal dermoid cyst secondary to myelomeningocele repair: a case report]. | Dermoid and epidermoid tumors arise from the invagination of epidermal elements into the neural tube during the embryonic period. However; many studies have reported that dermoid and epidermoid tumors occur 5 to 10 years after the first operation on myelomeningocele patients. A seven-year-old male with myelomeningocele which had been repaired in his neonatal period; presented leg pain and deterioration of gait disturbance and urinary incontinence. Neuroradiological examinations revealed a spinal dermoid cys | 10666735 | [
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[Spinal dermoid cyst secondary to myelomeningocele repair: a case report]. | t at the repaired myelomeningocele and tethered spinal cord. We removed the tumor and untethered the tethered spinal cord. Postoperatively; the patient's leg pain; gait disturbance and urinary incontinence improved immediately. Inappropriate surgical treatment of spina bifida may cause a second lesion; which leads to tethered-cord syndrome. We suggest that proper surgical treatment; early checkup and neuroradiological evaluations are very important for spina bifida patients who show signs of neurological de | 10666735 | [
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[Spinal dermoid cyst secondary to myelomeningocele repair: a case report]. | terioration. For the best treatment; neurological; urological and orthopedical follow-up study after the first repair operation is necessary. | 10666735 | [
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[A case of rete mirabile with congenital dysplasia of bilateral internal carotid arteries]. | We report a case of dysplasia of the congenital bilateral internal carotid arteries with the rete mirabile. The rete mirabile is not usually seen in the course of human growth; but it is a common finding in other mammals. Accordingly; some investigators have thought that the rete mirabile is "developmental shift". Our case has dysplasia of the bilateral internal carotid arteries (one is aplasia and the other is hypoplasia); but the patient had suffered from no ischemic symptom because her brain had been suf | 10666736 | [
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[A case of rete mirabile with congenital dysplasia of bilateral internal carotid arteries]. | ficiently fed by each of the rete mirabile. Angiographically; the frequency of the rete mirabile formation is about 1/10;000. There were 20 cases reported until 1997 (including our case). There were 5 cases (27.8%) with ischemic symptoms in spite of internal carotid artery dysplasia; 2 cases (11.1%) with intracerebral hemorrhage; 6 cases (33.3%) with subarachnoid hemorrhage (there were only two cases with aneurysm) and 5 cases without symptoms. We have tried to class the rete mirabile by angiographical find | 10666736 | [
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[A case of rete mirabile with congenital dysplasia of bilateral internal carotid arteries]. | ings. One is the M type finding resembling moyamoya vessels in stages 3 & 4 of moyamoya disease; and the other is the N type finding resembling a nidus of arteriovenous malformation. M type occurred in younger patients more often than N type; so M type may be the previous stage of N type. | 10666736 | [
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[Successful treatment using detachable coils for traumatic carotid cavernous fistula as a complication of transsphenoidal surgery for a pituitary adenoma: a case report]. | We report a case of a patient with traumatic carotid cavernous fistula (CCF) caused by transnasal-transsphenoidal surgery; who was successfully treated using detachable coils. A 47-year-old man was admitted to our hospital because of severe headache. He was confirmed to have a nonfunctioning pituitary adenoma with presellar-type sphenoid sinus. Cerebral angiography initially disclosed no vascular lesions. A transnasal-transsphenoidal adenomectomy was performed. When the anterior wall of the sphenoid sinus w | 10666737 | [
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[Successful treatment using detachable coils for traumatic carotid cavernous fistula as a complication of transsphenoidal surgery for a pituitary adenoma: a case report]. | as dissected with a chisel; the chisel deeply stuck into the posterolateral part of the sinus. Profuse arterial bleeding was observed through the sphenoid sinus. The bleeding was stopped easily by compression and packing with bone wax. The operation was continued; the sellar floor was opened widely and the tumor was removed subtotally. The medial wall of the cavernous sinus was intact. Histological examination revealed a pituitary adenoma. Immediately after surgery; the patient noticed a bruit. He developed | 10666737 | [
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[Successful treatment using detachable coils for traumatic carotid cavernous fistula as a complication of transsphenoidal surgery for a pituitary adenoma: a case report]. | chemosis and abducent palsy on the right side. Cerebral angiography displayed a high-flow CCF; which was attributed to the carotid artery injury caused by the transnasal-transsphenoidal surgery. The CCF disappeared after two-staged embolization using detachable coils; 1st transvenous and 2nd transarterial. Ten months later; cerebral angiography showed persistent occlusion of the fistula; and the patient experienced no tumor recurrence. It is suggested that drilling is a safer procedure than using a chisel | 10666737 | [
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[Successful treatment using detachable coils for traumatic carotid cavernous fistula as a complication of transsphenoidal surgery for a pituitary adenoma: a case report]. | for dissection of a sphenoid sinus with incomplete pneumatization. Endovascular treatment using detachable coils proved useful to manage the CCF; an unusual complication of transsphenoidal surgery. | 10666737 | [
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[Chronic subdural hematoma associated with malignancy: report of three cases]. | Three cases of chronic subdural hematoma (CSH) associated with malignancy are reported. Case 1; A one-year-old girl was referred for vomiting and convulsions. Left CSH was removed; and her symptoms disappeared. Cytological examination of chronic subdural hematoma revealed abnormal white blood cells. A clinical diagnosis of acute monocytic leukemia was made after the laboratory examination. Remission was achieved by chemotherapy; but she died one year after the operation. Case 2; A 72-year-old woman was refe | 10666738 | [
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[Chronic subdural hematoma associated with malignancy: report of three cases]. | rred for right hemiparesis and urinary incontinence. Left CSH was irrigated; and her clinical symptoms immediately disappeared. Cytological examination of chronic subdural hematoma revealed abnormal white blood cells. A clinical diagnosis of chronic lymphocytic leukemia was made after the laboratory examination. No treatment was given since there were no clinical symptoms of chronic lymphocytic leukemia. Case 3; A 70-year-old woman who had been affected with early gastric cancer and mammary cancer for the p | 10666738 | [
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[Chronic subdural hematoma associated with malignancy: report of three cases]. | revious two years was admitted to our clinic because of headache; right hemiparesis and consciousness disturbance. Left CSH was irrigated; and her clinical symptoms improved. However; there was a tendency to bleed because disseminated intravascular coagulation had occurred; and CT showed bilateral subdural hematoma. A second irrigation was performed; but her symptoms did not improve. Left acute subdural hematoma; which was removed by craniotomy; occurred three days after the second operation. Pathological e | 10666738 | [
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[Chronic subdural hematoma associated with malignancy: report of three cases]. | xamination of the outer membrane of the subdural hematoma revealed invasion of adenocarcinoma. She died three days after the third operation. It is recommended that both the cytological and the histological examinations be performed when possible; since they are simple to perform and very useful in some cases. | 10666738 | [
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[A case of spontaneous cervical carotid artery dissection managed by using primary stent placement]. | The authors report the case of a 35-year-old male who underwent stenting for spontaneous cervical carotid dissection. He presented with sudden onset of hemicrania and left facial palsy followed by left hemiparesis and dysarthria. On admission; carotid angiography revealed postsinus tapering occlusion of the right internal carotid artery. Initially; he was managed with conservative treatment; but his neurological status deteriorated. Findings of brain CT; MRI and IMP-SPECT suggested hypoperfusion of the righ | 10666739 | [
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[A case of spontaneous cervical carotid artery dissection managed by using primary stent placement]. | t cerebrum. A Palmaz stent; 39 mm in length; was successfully placed over the site of the dissection to restore normal patency through the dissected carotid artery. Following stent implantation; his neurological signs improved gradually but completely. Since the procedure; with oral administration of antiplatelet medication; he has suffered no cerebral ischemic events. Follow-up carotid angiography one year after stent implantation showed good patency of the stented segment. The present case emphasizes the | 10666739 | [
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[A case of spontaneous cervical carotid artery dissection managed by using primary stent placement]. | usefulness of stenting for spontaneous cervical carotid dissection. | 10666739 | [
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Chemical reduction of phosphate on the primitive earth. | If phosphorus played a role in the origin of life; some means of concentrating micromolar levels of phosphate (derived from the calcium phosphate mineral apatite); must first have been available. Here we show that simulated (mini)lightning discharges in model prebiotic atmospheres; including only minimally reducing ones; reduce orthophosphates; including apatite; to produce substantial yields of phosphite. Electrical discharges associated with volcanic eruptions could have provided a particularly suitable e | 10666740 | [
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Chemical reduction of phosphate on the primitive earth. | nvironment for this process. Production of relatively soluble and reactive phosphite salts could have supplied a pathway by which the first phosphorus atoms were incorporated into (pre)biological systems. | 10666740 | [
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Clay catalysis of oligonucleotide formation: kinetics of the reaction of the 5'-phosphorimidazolides of nucleotides with the non-basic heterocycles uracil and hypoxanthine. | The montmorillonite clay catalyzed condensation of activated monocleotides to oligomers of RNA is a possible first step in the formation of the proposed RNA world. The rate constants for the condensation of the phosphorimidazolide of adenosine were measured previously and these studies have been extended to the phosphorimidazolides of inosine and uridine in the present work to determine of substitution of neutral heterocycles for the basic adenine ring changes the reaction rate or regioselectivity. The olig | 10666741 | [
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Clay catalysis of oligonucleotide formation: kinetics of the reaction of the 5'-phosphorimidazolides of nucleotides with the non-basic heterocycles uracil and hypoxanthine. | omerization reactions of the 5'-phosphoromidazolides of uridine (ImpU) and inosine (ImpI) on montmorillonite yield oligo(U)s and oligo(I)s as long as heptamers. The rate constants for oligonucleotide formation were determined by measuring the rates of formation of the oligomers by HPLC. Both the apparent rate constants in the reaction mixture and the rate constants on the clay surface were calculated using the partition coefficients of the oligomers between the aqueous and clay phases. The rate constants fo | 10666741 | [
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