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Update cas9on.py (#3)
Browse files- Update cas9on.py (6664381aa6e96b09129eb2d71ff862196f7ae4e3)
Co-authored-by: Qingyang Liu <[email protected]>
cas9on.py
CHANGED
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@@ -83,7 +83,7 @@ def find_crispr_targets(sequence, chr, start, strand, transcript_id, exon_id, pa
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if i >= target_length:
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target_seq = sequence[i - target_length:i + 3]
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tar_start = start + i - target_length
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tar_end = start + i + 3
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gRNA = ''.join([dnatorna[base] for base in sequence[i - target_length:i]])
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targets.append([target_seq, gRNA, chr, str(tar_start), str(tar_end), str(strand), transcript_id, exon_id])
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if i >= target_length:
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target_seq = sequence[i - target_length:i + 3]
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tar_start = start + i - target_length
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+
tar_end = start + i + 3 - 1
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gRNA = ''.join([dnatorna[base] for base in sequence[i - target_length:i]])
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targets.append([target_seq, gRNA, chr, str(tar_start), str(tar_end), str(strand), transcript_id, exon_id])
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