Title: Fundamental Limits of Transfer Learning in Binary Classifications. Abstract: A critical performance barrier in modern machine learning is scarcity of labeled data required for training state of the art massive models, especially in quickly emerging problems with lack of extensive data sets or scenarios where data collection and labeling is expensive/time consuming. Transfer learning is gaining traction as a promising technique to alleviate this barrier by utilizing the data of a related but different \emph{source} task to compensate for the lack of data in a \emph{target} task where there are few labeled training data. While there has been many recent algorithmic advances in this domain, a fundamental understanding of when and how much one can transfer knowledge from a related domain to reduce the amount of labeled training data is far from understood. We provide a precise answer to this question for binary classification problems by deriving a novel lower bound on the generalization error that can be achieved by \emph{any} transfer learning algorithm (regardless of its computational complexity) as a function of the amount of source and target samples. Our lower bound depends on a natural notion of distance that can be easily computed on real world data sets. Other key features of our lower bound are that it does not depend on the source/target data distributions and requires minimal assumptions that enables it application to a broad range of problems. We also consider a more general setting where there are more than one source domains for knowledge transfer to the target task and develop new bounds on generalization error in this setting. We also corroborate our theoretical findings on real image classification and action recognition data sets. These experiments demonstrate that our natural notion of distance is indicative of the difficulty of knowledge transfer between different pairs of source/target tasks, allowing us to investigate the effect of different sources on the target generalization error. Furthermore, to evaluate the sharpness of our bounds we compare our developed lower bounds with upper-bounds achieved by transfer learning base-lines that utilize weighted empirical risk minimization on the combination of source(s) and target data sets.