Title: Measuring and mitigating interference in reinforcement learning. Abstract: Catastrophic interference is common in many network-based learning systems, and many proposals exist for mitigating it. But, before we overcome interference we must understand it better. In this work, we first provide a definition and novel measure of interference for value-based control methods such as Fitted Q Iteration and DQN. We systematically evaluate our measure of interference, showing that it correlates with forgetting, across a variety of network architectures. Our new interference measure allows us to ask novel scientific questions about commonly used deep learning architectures and develop new learning algorithms. In particular we show that updates on the last layer result in significantly higher interference than updates internal to the network. Lastly, we introduce a novel online-aware representation learning algorithm to minimize interference, and we empirically demonstrate that it improves stability and has lower interference.