how to make sequencing primers?
['Primer length should be in the range of 18 and 24 bases.', 'The primer should have a GC content of about 45-55%.', 'The primers should have a GC-lock (or GC "clamp") on the 3\' end (i.e. the last 1 or 2 nucleotides should be a G or C residue).']