DNA SEQUENCES AND PLASMIDS FOR THE PREPARATION OF PLANTS WITH CHANGED SUCROSE CONCENTRATIONDNA sequences are described, that by integration in a plant genome cause the activity of the sucrose-phosphate-synthase (SPS) of the plant to be changed, plasmids, containing these DNA sequences as well as transgenic plants that by introduction of the DNA sequences causes changes in the activity of sucrose-phosphate-synthase.DNA sequence with the coding region for sucrose-phosphate-synthase (SPS) from Solanum tuberosum for the preparation of plants with•modified sucrose concentration, characterised in that this sequence has the following nucleotide sequence (Seq. ID No.l):CTATTCTCTC CCCTCCTTTT TCTCCTCTCT TCAACCCCAA AACTTCCCTT TCAAAGCCTT 60TGCTTTCCCT TTCTCACTTA CCCAGATCAA CTAAGCCAAT TTGCTGTAGC CTCAGAAAAC 120AGCATTCCCA GATTGAAAAA GAATCTTTTT CAGTACCCAA AAGTTGGGTT TCTCATGTCC 180AGCAAGGATT AGCTGCTCTA GCTATTTCTT TAGCCCTTAA TTTTTGTCCA GTTGTGTCTT 240CTGATTCTGC ATTGGCATCT GAATTTGATG TGTTAAATGA AGGGCCACCA AAGGACTCAT 300ATGTAGTTGA TGATGCTGGT GTGCTTAGCA GGGTGACAAA GTCTGATTTG AAGGCATTGT 360TGTCTGATGT GGAGAAGAGA AAAGGCTTCC ACATTAATTT CATCACTGTC CGCAAGCTCA 420CTAGCAAAGC TGATGCTTTT GAGTATGCTG ACCAAGTTTT GGAGAAGTGG TACCCTAGTG 480TTGAACAAGG AAATGATAAG GGTATAGTTG TGCTTGTTAC AAGTCAAAAG GAAGGCGCAA 540TAACCGGTGG CCCTGATTTT GTAAAGGCCG TTGGAGATAC TGTTCTTGAT GCTACCGTCT 600CAGAGAACCT TCCAGTGTTG GCTACTGAAG AGAAGTACAA TGAAGCAGTT TTCAGCACTG 660CCACACGTCT TGTTGCAGCC ATTGATGGCC TTCCTGATCC TGGTGGACCC CAACTCAAGG 720ATAACAAAAG AGAGTCCAAC TTCAAATCCA GAGAGGAAAC TGATGAGAAA AGAGGACAAT 780SUBSTITUTE SHEET TCACACTTGT GGTTGGTGGG CTGTTAGTGA TTGCTTTTGT TGTTCCTATG GCTCAATACT 840ATGCATATGT TTCAAAGAAG TGAACTGTCT GATTCTGGAA AGTTACATTT TCGTGAGATT 900TGAGTAAGCA TGTATATTAT CGTGTACAAA ATGGTCCATT CGGAAATGAC TGATTC 956ATG AGA TAT TTA AAA AGG ATA AAT ATG AAG ATT TGG ACC TCC CCT 1001 Met Arg Tyr Leu Lys Arg He Asn Met Lys He Trp Thr Ser Pro 1 5 10 15AAC ATA ACG GAT ACT GCC ATT TCT TTT TCA GAG ATG CTG ACG CCA 1046 Asn He Thr Asp Thr Ala He Ser Phe Ser Glu Met Leu Thr Pro 20 25 30ATA AGT ACA GAC GGC TTG ATG ACT GAG ATG GGG GAG AGT AGT GGT 1091 He Ser Thr Asp Gly Leu Met Thr Glu Met Gly Glu Ser Ser Gly 35 40 45GCT TAT ATT ATT CGC ATT CCT TTT GGA CCA AGA GAG AAA TAT ATT 1136 Ala Tyr He He Arg He Pro Phe Gly Pro Arg Glu Lys Tyr He 50 55 60CCA AAA GAA CAG CTA TGG CCC TAT ATT CCC GAA TTT GTT GAT GGT 1181 Pro Lys Glu Gin Leu Trp Pro Tyr He Pro Glu Phe Val Asp Gly 65 70 75GCA CTT AAC CAT ATT ATT CAA ATG TCC AAA GTT CTT GGG GAG CAA 1226 Ala Leu Asn His He He Gin Met Ser Lys Val Leu Gly Glu Gin 80 85 90ATT GGT AGT GGC TAT CCT GTG TGG CCT GTT GCC ATA CAC GGA CAT 1271 He Gly Ser Gly Tyr Pro Val Trp Pro Val Ala He His Gly His 95 100 105SUBSTITUTE SHEET TAT GCT GAT GCT GGC GAC TCA GCT GCT CTC CTG TCA GGT GCT TTA 1316 Tyr Ala Asp Ala Gly Asp Ser Ala Ala Leu Leu Ser Gly Ala Leu 110 115 120AAT GTA CCA ATG CTT TTC ACT GGT CAC TCA CTT GGT AGA GAT AAG 1361 Asn Val Pro Met Leu Phe Thr Gly His Ser Leu Gly Arg Asp Lys 125 130 135TTG GAG CAA CTG TTG CGA CAA GGT CGT TTG TCA AAG GAT GAA ATA 1406 Leu Glu Gin Leu Leu Arg Gin Gly Arg Leu Ser Lys Asp Glu He 140 145 150AAC TCA ACC TAC AAG ATA ATG CGG AGA ATA GAG GCT GAA GAA TTA 1451 Asn Ser Thr Tyr Lys He Met Arg Arg He Glu Ala Glu Glu Leu 155 160 165ACT CTT GAT GCT TCC GAA ATT GTC ATC ACT AGT ACA AGA CAG GAG 1496 Thr Leu Asp Ala Ser Glu He Val He Thr Ser Thr Arg Gin Glu 170 175 180ATT GAC GAG CAA TGG CGT TTG TAT GAT GGG TTT GAT CCA ATA TTA 1541 He Asp Glu Gin Trp Arg Leu Tyr Asp Gly Phe Asp Pro He Leu 185 190 195GAG CGT AAG TTA CGT GCA AGG ATC AAG CGC AAT GTC AGC TGT TAT 1586 Glu Arg Lys Leu Arg Ala Arg He Lys Arg Asn Val Ser Cys Tyr 200 205 210GGC AGG TTT ATG CCT CGT ATG GCT GTA ATT CCT CCT GGG ATG GAG 1531 Gly Arg Phe Met Pro Arg Met Ala Val He Pro Pro Gly Met Glu 215 220 225SUBSTITUTE SHEET TTC CAC CAT ATT GTG CCA CAT GAA GGT GAC ATG GAT GGA GAA ACA 1676 Phe His His He Val Pro His Glu Gly Asp Met Asp Gly Glu Thr 230 235 240GAA GGA AGT GAA GAT GGG AAG ACC CCG GAT CCA CCT ATT TGG GCA 1721 Glu Gly Ser Glu Asp Gly Lys Thr Pro Asp Pro Pro He Trp Ala 245 250 255GAG ATT ATG CGC TTC TTT TCT AAT CCA AGG AAG CCT ATG ATA CTC 1766 Glu He Met Arg Phe Phe Ser Asn Pro Arg Lys Pro Met He Leu 260 265 270GCA CTT GCT AGG CCT GAT CCC AAG AAG AAC CTC ACT ACT TTA GTG 1811 Ala Leu Ala Arg Pro Asp Pro Lys Lys Asn Leu Thr Thr Leu Val 275 280 _ 285AAA GCA TTT GGT GAA TGT CGT CCA TTG AGA GAG CTT GCT AAT CTT 1856 Lys Ala Phe Gly Glu Cys Arg Pro Leu Arg Glu Leu Ala Asn Leu 290 295 300ACT TTG ATA ATG GGT AAT CGA GAT AAT ATC GAC GAA ATG TCT AGC 1901 Thr Leu He Met Gly Asn Arg Asp Asn He Asp Glu Met Ser Ser 305 310 315ACC AAT TCT GCA CTT CTT CTT TCA ATC TTG AAA ATG ATA GAT AAG 1946 Thr Asn Ser Ala Leu Leu Leu Ser He Leu Lys Met He Asp Lys 320 325 330TAT GAT CTT TAT GGT CAA GTA GCT TAT CCT AAA CAC CAC AAG CAG 1991 Tyr Asp Leu Tyr Gly Gin Val Ala Tyr Pro Lys His His Lys Gin 335 340 345TCA GAT GTT CCT GAT ATC TAC CGT CTT GCT GCA AAG ACT AAG GGT 2036 Ser Asp Val Pro Asp He Tyr Arg Leu Ala Ala Lys Thr Lys Gly 350 355 360SUBSTITUTE SHEET GTT TTT ATT AAT CCA GCT TTT ATT GAG CCT TTT GGA CTG ACT TTG 2081 Val Phe He Asn Pro Ala Phe He Glu Pro Phe Gly Leu Thr Leu 365 370 375ATT GAG GCA GCA GCT TAT GGT CTC CCA ATG GTA GCC ACA AAA AAT 2126 He Glu Ala Ala Ala Tyr Gly Leu Pro Met Val Ala Thr Lys Asn 380 385 390GGA GGA CCT GTT GAT ATA CAT AGG GTT CTT GAC AAT GGT CTC TTA 2171 Gly Gly Pro Val Asp He His A...
슈크로오스 농도가 변화된 식물의 제조를 위한 DNA 서열 및 플라스미드(DNA Sequences and Plasmids for Prepation of Plants with Changed Sucrose Concentration)본 발명은 식물 게놈 중으로 도입되어 식물의 슈크로오스-포스페이트-신타아제(SPS)의 활성을 변화시키는 DNA 서열 및 이들 DNA 서열을 함유하는 플라스미드 뿐만 아니라, DNA 서열이 도입되어 슈크로오스-포스페이트-신티아제의 활성이 변화된 유전자 전환 식물에 관한 것이다.하기의 뉴클레오티의 서열(서열 번호 1)을 가지는 것을 특징으로 하는, 슈크로오스 농도가 변화된 식물의 제조를 위한, 솔라늄 튜버로섬(Solanum tuberosum)으로부터의 슈크로오스-포스페이트-신타아제(SPS)의 코딩 영역을 가지는 DNA 서열: CTATTCTCTC CCCTCCTTTT TCTCCTCTCT TCAACCCCAA AACTTCCCTT TCAAAGCCTT 60 TGCTTTCCCT TTCTCACTTA CCCAGATCAA CTAAGCCAAT TTGCTGTAGC CTCAGAAAAC 120 AGCATTCCCA GATTGAAAAA GAATCTTTTT CAGTACCCAA AAGTTGGGTT TCTCATGTCC 180 AGCAAGGATT AGCTGCTCTA GCTATTTCTT TAGCCCTTAA TTTTTGTCCA GTTGTGTCTT 240 CTGATTCTGC ATTGGCATCT GAATTTGATG TGTTAAATGA AGGGCCACCA AAGGACTCAT 300 ATGTAGTTGA TGATGCTGGT GTGCTTAGCA GGGTGACAAA GTCTGATTTG AAGGCATTGT 360 TGTCTGATGT GGAGAAGAGA AAAGGCTTCC ACATTAATTT CATCACTGTC CGCAAGCTCA 420 CTAGCAAAGC TGATGCTTTT GAGTATGCTG ACCAAGTTTT GGAGAAGTGG TACCCTAGTG 480 TTGAACAAGG AAATGATAAG GGTATAGTTG TGCTTGTTAC AAGTCAAAAG GAAGGCGCAA 540 TAACCGGTGG CCCTGATTTT GTAAAGGCCG TTGGAGATAC TGTTCTTGAT GCTACCGTCT 600 CAGAGAACCT TCCAGTGTTG GCTACTGAAG AGAAGTACAA TGAAGCAGTT TTCAGCACTG 660 CCACACGTCT TGTTGCAGCC ATTGATGGCC TTCCTGATCC TGGTGGACCC CAACTCAAGG 720 ATAACAAAAG AGAGTCCAAC TTCAAATCCA GAGAGGAAAC TGATGAGAAA AGAGGACAAT 780 TCACACTTGT GGTTGGTGGG CTGTTAGTGA TTGCTTTTGT TGTTCCTATG GCTCAATACT 840 ATGCATATGT TTCAAAGAAG TGAACTGTCT GATTCTGGAA AGTTACATTT TCGTGAGATT 900 TGAGTAAGCA TGTATATTAT CGTGTACAAA ATGGTCCATT CGGAAATGAC TGATTC 956 ATG AGA TAT TTA AAA AGG ATA AAT ATG AAG ATT TGG ACC TCC CCT 1001 Met Arg Tyr Leu Lys Arg Ile Asn Met Lys Ile Trp Thr Ser Pro 1 5 10 15 AAC ATA ACG GAT ACT GCC ATT TCT TTT TCA GAG ATG CTG ACG CCA 1046 Asn Ile Thr Asp Thr Ala Ile Ser Phe Ser Glu Met Leu Thr Pro 20 25 30 ATA AGT ACA GAC GGC TTG ATG ACT GAG ATG GGG GAG AGT AGT GGT 1091 Ile Ser Thr Asp Gly Leu Met Thr Glu Met Gly Glu Ser Ser Gly 35 40 45 GCT TAT ATT ATT CGC ATT CCT TTT GGA CCA AGA GAG AAA TAT ATT 1136 Ala Tyr Ile Ile Arg Ile Pro Phe Gly Pro Arg Glu Lys Thr Ile 50 55 60 CCA AAA GAA CAG CTA TGG CCC TAT ATT CCC GAA TTT GTT GAT GGT 1181 Pro Lys GLu Gln Leu Trp Pro Tyr Ile Pro Glu Phe Val Asp Gly 65 70 75 GCA CTT AAC CAT ATT ATT CAA ATG TCC AAA GTT CTT GGG GAG CAA 1226 Ala Leu Asn His Ile Ile Gln Met Ser Lys Val Leu Gly Glu Gln 80 85 90 ATT GGT AGT GGC TAT CCT GTG TGG CCT GTT GCC ATA CAC GGA CAT 1271 Ile Gly Ser Gly Tyr Pro Val Trp Pro Val Ala Ile His Gly His 95 100 105 TAT GCT GAT GCT GGC GAC TCA GCT GCT CTC CTG TCA GGT GCT TTA 1316 Tyr Ala Asp ALa Gly Asp Ser Ala Ala Leu Leu Ser Gly Ala Leu 110 115 120 AAT GTA CCA ATG CTT TTC ACT GGT CAC TCA CTT GGT AGA GAT AAG 1361 Asn Val Pro Met Leu Phe Thr Gly His Ser Leu Gly Arg Asp Lys 125 130 135 TTG GAG CAA CTG TTG CGA CAA GGT CGT TTG TCA AAG GAT GAA ATA 1406 Leu Glu Gln Leu Leu Arg Gln Gly Arg Leu Ser Lys Asp Glu Ile 140 145 150 AAC TCA ACC TAC AAG ATA ATG CGG AGA ATA GAG GCT GAA GAA TTA 1451 Asn Ser Thr Tyr Lys Ile Met Arg Arg Ile Glu Ala Glu Glu Leu 155 160 165 ACT CTT GAT GCT TCC GAA ATT GTC ATC ACT AGT ACA AGA CAG GAG 1496 Thr Leu Asp Ala Ser Glu Ile Val Ile Thr Ser Thr Arg Gln Glu 170 175 180 ATT GAC GAG CAA TGG CGT TTG TAT GAT GGG TTT GAT CCA ATA TTA 1541 Ile Asp Glu Gln TrP Arg Leu Tyr Asp Glu Phe Asp Pro Ile Leu 185 190 195 GAG CGT AAG TTA CGT GCA AGG ATC AAG CGC AAT GTC AGC TGT TAT 1586 Glu Arg Lys Leu Arg Ala Arg Ile Lys Arg Asn Val Ser Cys Tyr 200 205 210 GGC AGG TTT ATG CCT CGT ATG GCT GTA ATT CCT CCT GGG ATG GAG 1631 Gly Arh Phe Met Pro Arg Met Ala Val Ile Pro Pro Gly Met Glu 215 220 225 TTC CAC CAT ATT GTG CCA CAT GAA GGT GAC ATG GAT GGA GAA ACA 1676 Phe His His Ile Val Pro His Glu Gly Asp Met Asp Gly Glu Thr 230 235 240 GAA GGA AGT GAA GAT GGG AAG ACC CCG GAT CCA CCT ATT TGG GCA 1721 Glu Gly Ser Glu Asp Gly Lys Thr Pro Asp Pro Pro Ile Trp Ala 245 250 255 GAG ATT ATG CGC TTC TTT TCT AAT CCA AGG AAG CCT ATG ATA CTC 1766 Glu Ile Met Arg Phe Phe Ser Asn Pro Arg Lys pro Met Ile Leu 260 265 270 GCA CTT GCT AGG CCT GAT CCC AAG AAG AAC CTC ACT ACT TTA GTG 1811 Ala Leu Ala Arg Pro Asp Pro Lys Lys Asn Leu Thr Thr Leu Val 275 280 285 AAA GCA TTT GGT GAA TGT CGT CCA TTG AGA GAG CTT GCT AAT CTT 1856 Lys Ala Phe Gly Glu Cys Arg Pro Leu Arg Glu Leu Ala Asn Leu 290 295 300 ACT TTG ATA ATG GGT AAT CGA GAT AAT ATC GAC GAA ATG TCT AGC 1901 Thr Leu Ile Met Gly Asn Arg Asp Asn Ile Asp Glu Met Ser Ser 305 310 315 ACC AAT TCT GCA CTT CTT CTT TCA ATC TTG AAA ATG ATA GAT AAG 1946 Thr Asn Ser Ala Leu Leu Leu Ser Ile Leu Lys Met Ile Asp Lys 320 325 330 TAT GAT CTT TAT GGT CAA GTA GCT TAT CCT AAA CAC CAC AAG CAG 1991 Tyr Asp Leu Tyr Gly Gln Val Ala Tyr Pro Lys His His Lys Gln 335 340 345 TCA GAT GTT CCT GAT ATC TAC CGT CTT GCT GCA AAG ACT AAG GGT 2036 Ser Asp Val Pro Asp Ile Tyr Arg Leu Ala Ala Lys Thr LyS Gly 350 355 360 GTT TTT ATT AAT CCA GCT TTT ATT GAG CCT TTT GGA CTG ACT TTG 2081 VaL phe Ile Asn Pro Ala Phe Ile Glu Pro Phe Gly Leu Thr Leu 365 370 375 ATT GAG GCA GCA GCT TAT GGT CTC CCA ATG GTA GCC ACA AAA AAT 2126 Ile Glu Ala Ala Ala Tyr Gly Leu Pro Met Val Ala Thr Lys Asn 380 385 390 GGA GGA CCT GTT GAT ATA CAT AGG GTT CTT GAC AAT GGT CTC TTA 2171 Gly Gly Pro Val Asp Ile His Arg Val Leu Asp Asn Gly Leu Leu 395 400 405 GTG GAT CCC CAT GAT CAG CAG GCA ATT GCT GAT GCT CTT TTG AAG 221...