Vocês realmente sabem fazer o cabeça de teia sentir especial!"

Elenco

 Josh Keaton - Peter Parker/Homem-Aranha
 Lacey Chabert - Gwen Stacy
 Joshua LeBar - Flash Thompson
 Grey DeLisle - Sally Avril/Betty Brant
 Alanna Ubach - Liz Allen
 James Arnold Taylor - Harry Osborn/Frederick Foswell
 Vanessa Marshall - Mary Jane Watson
 Clancy Brown - George Stacy
 Deborah Strang - Tia May
 Steve Blum - Duende Verde/Camaleão/Blackie Gaxton/Dillbert Trilby/Seymour O'Reilly
 Alan Rachins - Norman Osborn
 Andrew Kishino - Kenny 'King' Kong/Ned Lee
 Phil LaMarr - Robbie Robertson/Randy Robertson
 Daran Norris - J. Jonah Jameson/John Jameson
 Ben Diskin - Eddie Brock/Venom
 Peter MacNicol - Dr. Otto Octavius
 John DiMaggio - Cabeça de Martelo
 Kevin Michael Richardson - L. Thompson Lincoln/Lápide
 Dee Bradley Baker - Dr. Curt Connors
 Jeff Bennett - Montana/Shocker
 Kath Soucie - Martha Connors
 Brian George - Miles Warren
 Crispin Freeman - Electro
 Thomas F. Wilson - Stan Carter 
 Cree Summer - Glory Grant
 Robert Englund - Adrian Toomes
 Eric Lopez - Mark Allan
 Kelly Hu - Sha Shan Nguyen
 Thom Adcox-Hernandez - Phineas Mason
 Xander Berkeley - Quentin Beck/Mysterio
 Irene Bedard - Jean DeWolff
 Greg Weisman - Donald Menken 
 Dorian Harewood - Dr. Bromwell
 Tricia Helfer - Gata Negra
 Eric Vesbit - Kraven, O Caçador 
 Miguel Ferrer - Silvermane  
 Elisa Gabrielli - Dra.