Transport for London (TfL) announced a public consultation process to decide how the city's private hire trade should be regulated.
Proposals include improving the driving and English language skills of drivers and stricter insurance requirements.
But Uber said that the proposals would raise prices and add to congestion in London.
"It's meant to be a public consultation but it clearly hasn't got the public's interests at heart," said Gareth Mead, Uber Europe's spokesperson on the Today programme.
An online petition called called Support Uber London has received more than 123,000 signatures, stating that the proposals would lead to a five minute waiting time before a customer can begin their Uber journey.
"There will be a mandatory five minute wait time, even if a car is available just around the corner. You won't even be able to see the nearest cars when you open the app", the petition claimed.
Mr Mead said of the proposed five-minute wait for trip confirmation: "As the night's draw in, it's starting to get colder and darker. I'm not quite sure how that [proposal] benefits anybody."
Earlier this week Garrett Emmerson, a senior executive at TfL, said in a statement: "In recent years the private hire industry has grown exponentially and technology has also developed rapidly. The consultation sets out a number of ways that standards across the industry could be raised.
"No final decisions have been made and we're keen to hear a range of views from the trade and from Londoners too."
The Californian technology company has faced resistance from taxi drivers around the world, who argue that Uber drivers escape regulation.
But Mr Mead said Uber drivers are subject to the same measures which govern the private hire industry, including background checks and licensing requirements.
Responding to criticism that Uber cannot ensure that its drivers are taking rest breaks, Mr Mead said: "One of the advantages of a digital technology company is that you can see, to the minute, what they [drivers] are doing, and we tell them when they need to take a break."
Mr Mead said that since Uber launched in London a year ago, it had gained 1 million users in the capital.

It happened at the Mill Street level crossing in Leominster, Herefordshire, at 05:50 GMT on Friday.
The nearby A44 is likely to be closed all day, Hereford and Worcester Fire and Rescue service said. Rail services between Hereford and Shrewsbury are expected to be disrupted for most of the day.
No-one was injured in the crash.
Follow the latest on this and other stories in Herefordshire and Worcestershire here
A specialist firm was brought in to help move the lorry, which was left leaning at an angle against the house.
The house is damaged but inspections are still going on to see how bad the situation is, Martin Lown from Hereford & Worcester Fire and Rescue Service said.
"We've got a structural engineer on site and as the recovery continues we will monitor the situation," he said.
Replacement buses are running between Shrewsbury and Hereford.

Southern Railway luggage van No 2464 has spent four months being stripped and repainted at Locomotion, the National Railway Museum in Shildon.
The carriage transported Churchill's coffin from London to Oxfordshire in 1965.
Manager Gary Campbell, said the restoration was something a lot of people in the town were "proud of".
The newly restored carriage, which cost about Â£30,000, will now move to the National Railway Museum in York.
It will be joined by the locomotive which pulled it as part of an exhibition commemorating the 50th anniversary of the former Prime Minister's state funeral.
After the funeral in London, his coffin was loaded into the carriage which was then drawn by Battle of Britain locomotive Winston Churchill to Oxfordshire.
Thousands gathered along the route to pay their respects.
Churchill was laid to rest in the parish churchyard of Bladon, close to Blenheim Palace, where he was born 89 years earlier.
The carriage was built in 1931 and was used during World War Two on evacuation trains before being put into Pullman colours in the 1960s.
It was moved to Los Angeles in 1966 but returned to the UK in 2007 where it entered the Swanage Railway Collection.
Taxi hiring app Uber said London's transport authority is proposing rules that are "against the public interest".
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A lorry carrying 28 tonnes of grain has crashed into a house next to a level crossing.
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The carriage which carried Sir Winston Churchill's coffin to his final resting place has been restored.