Laura Matthews, 29, was told her signature - "L. Skywalker" - infringed a trademark in July this year.
She was issued with the document several weeks later, after threatening to take legal action.
But she has now been told the passport was issued in error and has been cancelled.
Ms Matthews told the BBC the decision has left her feeling "upset, frustrated and disappointed".
A Home Office spokesman said: "Protecting the integrity of the UK passport is vital and we do not accept a change of name for frivolous reasons."
In a letter to Ms Matthews, the Passport Office said its "published guidance provides that we will refuse to recognise a change of name... if it is made for a bet or frivolous purpose.
"Additionally, in your case, the signature used in the passport is different to your surname and by your own admission was done for 'a laugh'".
"Regrettably, an error was made in issuing your passport," the letter said.
"I am sorry that the error was made but I have to inform you that your passport will be cancelled."
Ms Matthews, from Southend in Essex, added the middle name using a deed poll in 2008 "for a bit of fun".
She had not renewed her passport since adding the moniker, and experienced several weeks of delays when she tried to do so in April.
She was told the delays were because her signature "infringed a trademark".
Her passport was issued at the end of August after she contacted a law firm specialising in intellectual property law and said she would take legal action unless her application was approved.
Ms Matthews said this was the fourth time the Passport Office had changed its mind about her case.
"Now I'm not sure if it's my signature they have a problem with, or my middle name," she said.
"All I know it's extremely disappointing that I've been let down again by an organisation which doesn't seem to know if it's coming or going.
"I don't think I'm going to be able to fight it. I don't know what else I can do."
The Home Office spokesman added: "We have apologised to the applicant and agreed to meet the cost of a new passport in a name that meets the published guidance."

Sarah Johnson was one of 21 women heading to Liverpool when their minibus was hit by a lorry on the M62.
Her friend Bethany Jones, 18, was killed while Ms Johnson and several others were badly hurt.
Minibus driver James Johnson was jailed for more than six years for causing Bethany's death, in April 2013.
Ms Johnson, who broke her shoulder, back and pelvis, said the help she received from a charity while in hospital led her to want to support others.
Speaking publicly for the first time about the crash, Ms Johnson described how everyone was "excited and giddy" for the hen party.
"To me the impact was just a massive explosion," she said.  "I thought the bus had blown up.
"I remember the bus dropping on its side. The next thing, I woke up on the roadside so I'd actually come out of the window."
Ms Johnson was taken to Leeds General Infirmary where she, along with Bethany's sister Amy Firth, underwent major surgery and spent time in intensive care.
Whilst she was there she got support from charity Day One, which helps victims of major trauma.
She said: "It's absolutely fantastic.
"It supports people by giving benefit advice, legal advice and peer support such as me and Amy, who have been in similar situations and who are now helping other people who've suffered from major trauma."
Ms Johnson said the crash had made her realise how lucky she had been.
"Beth can't complain, she's not here," she added.  "We just have to be grateful for what we've got."

The 27-year-old woman was found at Knowle Close, Redditch, after police were called early on Sunday.
The arrested man, a 32-year-old from Redditch, is in police custody.
The results of a post-mortem examination on the woman, who has not been formally identified, will be released later, a West Mercia Police spokesperson said.
Updates on this story and more from Worcestershire
A woman who added Skywalker as a middle name has had her passport cancelled after being told her application was "frivolous".
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A woman who was seriously hurt in a fatal hen party motorway crash is now helping other major trauma victims rebuild their lives.
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A man has been arrested on suspicion of murder after a woman was found dead at a house in Worcestershire.