Marineland, in Niagara Falls, was also charged with failing to provide adequate food and water for 35 bears, the Ontario Society for the Prevention of Cruelty to Animals (OSPCA) said.
Three final charges relate to failing to comply with the prescribed standards of care for a peacock and guinea hens.
Marineland deny the allegations.
The company told the BBC the accusations were made by "a former animal care worker who was fired for poor performance and inappropriate behaviour".
It said the peacock - "one among thousands of birds" - had a problem with its eye but was otherwise healthy. It also blamed the OSPCA staff for upsetting the guinea hens and said the concerns over the bears' food were due to some small labels being left on pieces of fruit.
The park spokesman added there had never been concerns raised about the bears' access to water.
However, the OSPCA, which has not removed any animals but is continuing to monitor the situation, says further charges are likely to follow.
Spokesman Alison Cross said: "If convicted, they could face a CA$60,000 ($44,300/Â£35,500) fine, a lifetime ban in owning animals and up to two years in jail."

The 25-year-old was hit by the motorbike after several riders came down in a crash as the Gent-Wevelgem race passed through northern France.
Jose Been, spokeswoman for the Wanty-Gobert team, told BBC Sport it was "not a case of the motorcyclist going too fast and causing the crash".
Demoitie was taken to hospital in Lille but died some time later.
A French police inquiry is under way and the sport's governing body, the UCI, is co-operating with the investigation.
Demoitie was among several competitors involved in a fall in the one-day race, which was won by world champion Peter Sagan.
"The cyclists fell right in front of the motorcyclist who had no time to react and just hit them," Beem said.
"The motorcyclist is very experienced and has been in the races a long time. He is as grief-stricken as we are.
"It was a very unfortunate, horrible accident for both sides."
At a news conference on Monday, tearful team manager Jean-Francois Bourlart said Demoitie rode his first WorldTour race - the E3 Harelbeke in Belgium - last week.
"He was very proud of himself and we couldn't have been more proud of him," he said.
"On Sunday, the incredible happened, the inconceivable. We can't believe it. We are angry. We cry and we think of his wife Astrid and his family."
Earlier on Monday, Professional Cyclists' Association president Gianni Bugno, a former rider, said: "I do not want to accuse anyone, but make everyone reflect on the responsibility we have to ensure... a very high level of attention, awareness, and control over safety standards during each race."
Several high-profile professional cyclists have paid tribute to Demoitie, while others have questioned whether there needs to be rule changes around motorbikes, which are used mainly for security and media.
In a Facebook post, German sprinter Marcel Kittel said the sport had reached a "new and very sad low point in the history of cycling and safety".

The firm said market conditions "continued to be challenging" but that they anticipate a return to growth in 2014.
Northstone showed a pre-tax profit of ??6m on turnover of ??239m, compared to 2012 when profit of ??8m was achieved on a ??299m turnover.
Its best-known trading business is the Farrans construction firm.
It also sells quarry products and ground access systems for the utility industries.
The fall in group turnover in 2012 was all attributable to its building/civil engineering division where turnover fell from ??226m to ??153m.
The company's workforce fell from 992 to 968 over the year and the accounts show redundancy costs of ??358,000.
During the year, the firm bought the Cemex (NI) cement business for an undisclosed sum.
Northstone is ultimately owned by CRH plc, the Dublin-based building materials group which is worth around ??10bn.
A Canadian wildlife park has been charged with five counts of animal cruelty - including allowing a peacock to be in distress.
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A crash with a motorbike which killed Belgian cyclist Antoine Demoitie was an "unfortunate accident", says his team.
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Northstone, one of Northern Ireland largest construction groups, reported reduced turnover and profits in 2013.