All 42 of its member clubs are expected to take strict disciplinary measures against fans who indulge in anti-social behaviour during matches.
The updated guidance, which comes into force immediately, states that home clubs are responsible for "good order and security".
Clubs are also urged to step up efforts to identify culprits.
Under the previous rules, clubs could argue that they had taken all practical steps to deter misbehaviour inside their stadiums.
Now they must been seen to actively pursue cases and take "appropriate" action against the perpetrators.
In June, following disorder at the Scottish Cup final, Justice Secretary Michael Matheson called for "a transparent and robust scheme" to prevent and deal with unacceptable conduct.
He went on to warn: "The Scottish government is prepared to act if Scottish football isn't."
On the rule changes, SPFL chief executive Neil Doncaster said: "The SPFL and its member clubs are committed to preventing and to addressing unacceptable conduct where it arises, to ensure our stadiums are friendly, welcoming and safe environments where all supporters can enjoy Scottish football.
"This ongoing work includes this updated guidance for clubs which sets out the reasonably practicable measures that member clubs can take to address this issue and to identify and sanction those who engage in unacceptable conduct.
"It has been fully consulted on with all 42 clubs, the Scottish FA and the Scottish Government and, indeed, dialogue continues with the Government on a number of further measures which will be discussed early this year."

10 September 2016 Last updated at 18:36 BST
The house of Pietro's owner collapsed as a result of the quake. The cat was taken to a veterinary clinic and is said to be in a critical condition.
He was found when his owners returned to the house to get some belongings.
Nearly 300 people died in August's magnitude-6.2 earthquake.
Read more
Tales of survival
Before and after images show destruction

The review of 5,000 studies on golf and wellbeing found physical benefits increased with the player's age.
The study is part of the Gold and Health Project, which is led by the World Golf Foundation.
The project plans to look at gaps in the research such as golf's links to mental health and muscle strengthening.
Executive director of the Golf and Health project Dr Roger Hawkes said the aim had been to add some "scientific rigour" to statements made about golf and wellbeing.
"For a number of years we've felt we've underplayed the likely benefits of golf on peoples' health," he said.
"We already have a scoping review, outlining what we know already, and we're going to look at that research and the gaps in knowledge so that in five years time we will have really good evidence."
Having this academically firmed up through in-depth research is going to provide real, tangible resource
Lead researcher Dr Andrew Murray said: "Evidence suggests golfers live longer than non-golfers, enjoying improvements in cholesterol levels, body composition, wellness, self-esteem and self-worth.
"Given that the sport can be played by the very young to the very old, this demonstrates a wide variety of health benefits for people of all ages."
The study suggested playing golf could help players meet and exceed minimum government recommendations for moderate to vigorous physical activity.
It claimed that golfers walking 18 holes could cover four to eight miles, while those using an electric golf cart typically chalked up four miles.
Players burned a minimum of 500 calories over 18 holes, it said.
World Golf Foundation CEO Steve Mona said the Golf and Health Project was vital to the development of the sport.
"This project is something we can all get behind, as it is universally agreed that golf is good for you," he said.
"Having this academically firmed up through in-depth research is going to provide real, tangible resources that can be used by governments and politicians, professional tours, governing bodies, golf businesses, PGA Professionals and more - all to the sport's benefit."
The research was released to coincide with the Alfred Dunhill Links Championship in St Andrews this week.
For more information on getting into golf, take a look at the Get Inspired activity guide on the sport.
The Scottish Professional Football League has issued new regulations aimed at tackling supporter misconduct.
[NEXT_CONCEPT]
A cat has been rescued from the rubble of a house 16 days after an earthquake hit the Italian city of Amatrice.
[NEXT_CONCEPT]
A regular game of golf is likely to increase life expectancy and lead to better physical health, according to University of Edinburgh researchers.