Guiseley managed to frustrate Dover during a first half that was dominated by the hosts, but Dover kept plugging away and took complete control just after the break.
Ricky Miller underlined his position as the National League's top marksman by slotting his 19th of the season after 46 minutes, beating two defenders before firing hard and low past Jonathan Maxted.
Moses Emmanuel profited from slack defending three minutes later to make it comfortable for the hosts, who remain sixth and one point off the play-off places.
Report supplied by Press Association.
Match ends, Dover Athletic 2, Guiseley 0.
Second Half ends, Dover Athletic 2, Guiseley 0.
Substitution, Dover Athletic. Aswad Thomas replaces Sam Magri.
Substitution, Guiseley. Michael Rankine replaces Adam Boyes.
Substitution, Guiseley. Joel Logan replaces Jordan Preston.
Goal!  Dover Athletic 2, Guiseley 0. Moses Emmanuel (Dover Athletic).
Goal!  Dover Athletic 1, Guiseley 0. Ricky Miller (Dover Athletic).
Second Half begins Dover Athletic 0, Guiseley 0.
First Half ends, Dover Athletic 0, Guiseley 0.
Ashley Palmer (Guiseley) is shown the yellow card.
Connor Brown (Guiseley) is shown the yellow card.
Moses Emmanuel (Dover Athletic) is shown the yellow card.
Ricky Miller (Dover Athletic) is shown the yellow card.
Danny East (Guiseley) is shown the yellow card.
First Half begins.
Lineups are announced and players are warming up.

Comley, 20, has played twice for the R's first-team and signed a two-year deal at Loftus Road in May, having been with the club since the age of nine.
His full debut came as a substitute in last season's 5-1 Premier League defeat by Leicester City.
"We like what we've seen from Brandon and we think we can work with him," Carlisle boss Keith Curle said.
The Islington-born midfielder, who featured in a trial match for Carlisle against Barrow, will return to his parent club on 20 February.

Imran Khan, 33, is accused of taking the girl from Nairne Street in Burnley, Lancashire, at 16:00 GMT on Thursday.
Khan, of Pendle Street, Accrington, has also been charged with breaching a sexual offences prevention order and inciting a girl under 13 to engage in sexual activity.
He was remanded in custody by Burnley magistrates.
He is due to face the charges at the town's crown court on 23 April.

Kingfisher House, near the domestic airport in the western city of Mumbai (Bombay), was being sold by lenders to recover losses.
However, bidders appeared to be deterred by the asking price of 1.5bn rupees ($22m; Â£15m).
The businessman is said to have some $1.4bn of unpaid debts after his Kingfisher airline collapsed in 2013.
Speculation rose about his whereabouts after a consortium of banks and creditors approached the Supreme Court over  unpaid debts.
They demanded that his passport be impounded, and that $75m in severance pay he was to receive from Diageo be blocked and used to repay them.
The payout was blocked, but it emerged that Mr Mallya had already left India.
Later in a series of tweets he denied that he was "absconding" as was being alleged in India.
Media reports say Mr Mallya is currently living in London.
Opposition MPs have demanded that the government bring him back to face the law.
Mr Mallya, who is a household name in India, sold a large chunk of United Spirits, which he inherited from his father, to Diageo in April 2014.
He was to receive the $75m payout as settlement after being ousted from the firm in February.
Mr Mallya, once dubbed by Indian media as "India's Richard Branson" and the "King of Good Times" for his lavish lifestyle, also owns a stake in the Formula One team Force India.
Dover kept up the pressure on those on in the National League playoff places with a comfortable 2-0 win over strugglers Guiseley at the Crabble Athletic Ground.
[NEXT_CONCEPT]
Carlisle United have signed midfielder Brandon Comley from from Queens Park Rangers on a one-month youth loan.
[NEXT_CONCEPT]
A man has been charged with the abduction of a six-year-old girl who was taken in a car.
[NEXT_CONCEPT]
An auction to sell a property belonging to Indian drinks baron Vijay Mallya has failed to attract any bidders.