The<unk> -UAS ectopic expression<unk>  is widely used in the fruit fly, Drosophila melanogaster, for the overexpression of transgenes. This<unk>  operates under the assumption that the yeast transcription factor,<unk> , is inactive in D. melanogaster. This bipartite (two-part) expression<unk>  utilizes the yeast transcription factor<unk>  and its target sequence<unk>  (upstream activation sequence), to which<unk>  binds in order to activate gene transcription.<unk>  can be expressed in many different patterns by placing it under the control of various D. melanogaster promoter sequences. Since<unk>  promoter sequences (CGGAGTACTGTCCTCC) are not found in D. melanogaster, it is assumed that<unk>  is inactive. This is very useful for the overexpression of transgenes from a<unk>  promoter because<unk>  will drive expression of the transgene while not otherwise affecting the cells.<unk> -UAS expression<unk> s are used to artificially express genes in organisms carrying both constructs.  This is achieved by crossing a driver line with a<unk>  line, with the gene fused to<unk>  being expressed in any time and location the driver gene would normally be expressed.
GAL4/UAS system
Triage tag
Transcriptome
Bacterial artificial chromosome
Short tandem repeat
TORCH complex
Cytome
Cre-Lox recombination