<unk>  is an enzyme that mediates the process of DNA replication in prokaryotes.<unk>  was the first enzyme discovered with polymerase activity, and it is the best characterized enzyme. Although this was the first enzyme to be discovered that had the required polymerase activities, it is not the primary enzyme involved with bacterial DNA replication. That enzyme is<unk> II<unk> II). It is 928 residues long, and an example of a processive enzyme - an enzyme which catalyzes a series of polymerisations. Discovered by Arthur Kornberg in 1956, it was the first known<unk> and, indeed, the first known polymerase of any kind), and was initially characterized in E. coli, although it is ubiquitous in prokaryotes. It is often referred to as simply<unk> . In E. coli and many other bacteria, the gene which encodes<unk>  is known as polA.<unk>  possesses three enzymatic activities:<unk>  removes the RNA primer from the lagging strand and fills in the necessary nucleotides of the Okazaki fragments (see DNA replication) in 5' -> 3' direction, proofreading for mistakes as it goes. It is a template-dependent enzyme, as it only adds nucleotides that correctly base pair with an existing DNA strand acting as a template. Ligase then joins the various fragments together into a continuous strand of DNA.
Despite its early characterisation, it quickly became apparent that<unk>  was not the enzyme responsible for most DNA synthesis — E. coli DNA replication proceeds at approximately 1,000 nucleotides/second, while the rate of synthesis by<unk>  averages only 20 nucleotides/second. Moreover, its cellular abundance of approximately 400 molecules per cell did not correlate with the fact that there are typically only two replication forks in E. coli.  Moreover, it is insufficiently processive to copy an entire genome, as it falls off after incorporating only 25-50 nucleotides. This was proven when, in 1969, John Cairns isolated a viable<unk>  mutant that lacked the polymerase activity. Cairns' lab assistant Paula De Lucia created thousands of cell free extracts from E.coli colonies and assayed them for DNA-polymerase activity. The 3,478th clone contained the polA mutant, which was named so by Cairns to credit "Paula" [De Lucia]. It was not until the discovery of<unk> II that the main replicative<unk> as finally identified.
DNA polymerase I
Ligase
RNA polymerase
DNA glycosylase
Deoxyribonuclease
Restriction enzyme
Methyltransferase
Histone deacetylase