<unk>  polyacrylamide gel electrophoresis is an electrophoretic separation method typically used in proteomics.<unk>  should not be confused with SDS-PAGE. SDS-PAGE uses a detergent, sodium dodecyl sulfate, to denature proteins. The primary effect is that proteins in protein complexes are separated, reducing the dependence of protein mobility on folding.
In contrast,<unk>  separations are run in non-denaturing conditions. Detergents are used only to the extent that they are necessary to lyse lipid membranes in the cell. Complexes remain--for the most part--associated and folded as they would be in the cell. One downside, however, is that complexes may not separate cleanly or predictably, since they cannot move through the polyacrylamide gel as quickly as individual, denatured proteins.
There are three popular methods of<unk> , clear<unk>  (CN-PAGE), blue<unk>  (BN-PAGE), and quantitative preparative<unk>  continuous (QPNC-PAGE).
Native PAGE
Electrophoresis
SDS-PAGE
Nucleic acid hybridization
Dot blot
Western blot
DNA sequencing
Polymerase chain reaction