<unk>  is a molecular biology technique in which a mutation is created at a defined<unk>  in a DNA molecule, usually a circular molecule known as a plasmid. In general,<unk>  requires that the wild-type gene sequence be known.
This technique is also known as<unk> -specific<unk>  or Oligonucleotide-directed<unk> .
Site-directed mutagenesis
Molecular cloning
Triage tag
DNA sequencing
Short tandem repeat
Nucleic acid hybridization
Ligase chain reaction
Sequencing