<unk>  or 2-hybrid<unk>  is a molecular biology technique used to discover protein-protein interactions and protein-DNA interactions by testing for physical interactions (such as binding) between<unk>  proteins or a single protein and a DNA molecule, respectively.
The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For the purposes of<unk> , the transcription factor is split into<unk>  separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for activation of transcription.
Two-hybrid screening
Nucleic acid amplification technique
Molecular cloning
Southern blot
Nucleic acid hybridization
DNA sequencing
Shotgun sequencing
BET theory