Aduro Biotech , Inc. Protocol ADU -CL-02-v8 CRS -207 with Pemetrexed and Cisplatin Version 8, 08 March 2018 Page 47 Processing and Storage • PBMCs will be processed within 6 hours post -collection using Ficoll- Hypaque centrifugation. Isolated PB MCs will be stored in liquid nitrogen. Plasma collected from the PBMC process will be aliquoted and stored at - 70°C or colder. • Serum will be processed within 6 hours post -blood draw from a 10 mL serum tube. The serum samples will be stored at -70°C or colder. • The detailed procedures for PBM C and serum processing will be provided in a laboratory manual. Training will also be provided and only trained, qualified personnel will process samples. • Samples will be processed and stored at each site locally. At NCI, samples will be processed and stored by the Molecular Pharmacology Program (Building 10, Room 5A01; William Figg, Pharm.D.) Testing Site s • Cellular mesothelin - and Lm-specific immunity will be assessed by IFN -γ ELISPOT analysis as previous ly described 26. Additionally, serum cytokines/chemokines will be assessed 20 to 26 hours following each CRS - 207 infusion. Samples for ELISPOT and cytokine/chemokine analysis will be stored locally and batch -shipped in liquid nitrogen to Aduro for testing. • Antibody specific to mesothelin will be tested by ELISA as previously described 22. Antibody testing will be conducted at NCI at the laboratory of Dr. Raffit Hassan, M.D (Building 37, Room 5108). 5.3.2 Tumor Markers Tumor markers such as mesothelin, CA -125 and osteopontin will be monitored before, during and after the treatment course. Serum will be collected to determine circulating levels of mesothelin and CA -125, two tumor markers that have been demonstrated to be elevated in subjects with pleural mesothelioma. Systemic levels of osteopontin will be determined from plasma by a commercially -available ELISA. CA-125 will be collected as per local laboratory standards. Mesothelin and Osteopontin Sample Collection Aduro Biotech , Inc. Protocol ADU -CL-02-v8 CRS -207 with Pemetrexed and Cisplatin Version 8, 08 March 2018 Page 48 Samples for mesothelin testing will be draw n in a 5 mL red top tube and s amples for osteopontin testing will be drawn in a 5 mL green top tube before during and after treatment as indicated in the study calendar. At NCI site, p lease page 102 -11964 (Gareth Peters or alternate tech nician from Dr.