4. Quantitative ELISA for the detection of CMV antibodies and antibody avidity : 96 well Immulon 4 plates (Dynatech Laboratories) are incubated with CMV lysate (Virusys) at a concentration of 7.5 μg/ml diluted in carbonate alternating with rows of PBS/1% BSA. Wells set up as a standard curve are incubated with purified 6304p Protocol 118 04/06/2016 h u m a n I g G ( S i g m a B i o S c i e n c e s , S t .