Should a CRIS screen not be ava ilable, the CRIS downtime proced ures will be followed. Samples will not be sent outside NIH without IRB notification a nd an executed MTA. SAMPLES SENT TO FIGGLAB xVenous blood samples will be colle cted in either a 4 mL or an 8 mL SST tube to be processed for serum and stored f or future research. Record the date and exact time of draw on the tube. Blood tubes may be kept in the refrigerator u ntil pick up. xFor sample pick up, page 102-11964. xFor immediate help, call 240-760-6180 (main blood processing core number) or, if no answer, 240-760-6190 (main clini cal pharmacology lab number). xFor questions regarding sample processing, contact Julie Barnes by e-mail or at 240-760- 6044. xThe samples will be processed, barcoded, and stored in Dr. Figg ’s lab until requested by the investigator. SAMPLES SENT TO SURGERY BRANCH CELLPRODUCTION FACILITY xVenous blood samples will be colle cted in 8 mL CPT tubes to be pr ocessed and stored for future research. Record the dat e and exact time of draw on the tube. Blood tubes are kept at room temperature until pick up. xSamples will be picked up by the research nurse or designee and transported to the SB CPF within 24 hours of blood draw. xThe samples will be processe d, barcoded, and stored in the SB C PF. PRIOR TO EACH VACCINATION x8 CPT tubes (8 mL each): SB CPF x1 SST tube (8 mL): Figg Lab ATEACH SCHEDULED FOLLOW -UP EVALUATION x8 CPT tubes (8 mL each): SB CPF x1 SST tube (8 mL): Figg Lab IMMUNOLOGICAL TESTING xA variety of tests including e valuation of specific lysis and c ytokine release, metabolomic and bioenergetic studies (using Seahorse), intracellular FACS o f cytokine production, Abbreviated Title: Personalized DC Vaccine Version Date: March 26, 2018 25ELISA-spot assays, and lymphocyte subset analysis may be used t o evaluate the immunological correlates of trea tment. In general, differences of 2-3 fold in these assays over the baseline measurement are indicative of true biologic dif ferences.