org/ 10. 1186/ s12943- 021- 01467-8. Additional file 1: Supplementary data. Additional file 2: Supplementary Figure S1. The profiling of DNA muta- tion and RNA editing identified in patient N18. Additional file 3: Supplementary Figure S2. The Ex vivo IFN-γ ELISPOT responses for PBMCs stimulated by personalized neoantigen pools of patient N06 during neoantigen vaccinations. Additional file 4: Supplementary Figure S3. The Ex vivo IFN-γ ELISPOT responses for PBMCs stimulated by personalized neoantigen pools of patient N13 during neoantigen vaccinations. Additional file 5: Supplementary Figure S4. The Ex vivo IFN-γ ELISPOT responses for PBMCs stimulated by individual neoantigen peptide after neoantigen vaccinations (5 months) in 7 patients. The peptide in blue font, orange font and green font indicated as pool1, pool2, and pool3, respectively.