................................................... 44 Figure 1: A Process for the Id entification of Immunogenic Somat ic Mutations.......... 44 Figure 2: TLR Mix Induces the Secretion of IL-12p70, TNF Dand Multiple Chemokines .............................................................................................................................. 45 Figure 3: TLR Mix Alone is Sufficient to Induce Potent DC Matur ation.................... 46 Figure 4: Long Peptides, Minima l Epitopes and TMG mRNA used to Evaluate Antigen Presentation for CD 4 and CD8 Cells............................. ........................................................... 47 Figure 5: Long Peptides and Minima l Epitopes are Superior to TM G mRNA in Presenting Antigens to CD4 and CD8 Cells....................... ...................................................... 48 Figure 6: Peptide Loaded, TLR Stimulated DC’s Efficiently Prese nt Both CD4 and CD8 Epitopes............................................................................................................................ 49 Figure 7: Overview of the Met hod used to Evaluate the Activation of Neoantigen Specific T Cells......................................................................................................................... 50 Figure 8: DCs Matured with TLR Mix are Capable of Stimulating N aïve, Antigen- Experienced and Terminally Differentiated Effector Cells ....... ...............................................