Randomized Test Drug kit with Patient ID number. This kit contains either a single 1.0 mL vial of E75 acetate solution (1.5 mg/mL) or a single 1.0 mL vial of placebo. 2. Lyophilized Leukine kit with Patient ID number. This kit contains a single vial of lyophilized Leukine (250 μg). 3. Ancillary Supplies: 7-1mL TB syringes, 5-3/8 inch 26G needles, 4-2 inch 21G needles, 2-3 mL luer lock syringes. Dose Preparation: NOTE: DO NOT INVERT ANY STUDY DRUG VIALS DURING PREPARATION 1. Remove cap from vial of SWFI and wipe septum with alcohol prep pad. 2. Remove cap from vial of lyophilized Leukine (250 μg) and wipe with alcohol prep pad. 3. Attach a 2 inch 21G needle to a 1.0 mL TB syringe and draw up 1.0 mL from the vial of SWFI. 4. Using the needle and syringe from step #3, insert the needle into the Leukine vial. 54 Protocol: 2014-0443 Version 2.1 Version Date: 11 April 2016 • Angle the needle to the side of the Leukine vial and slowly expel 1.0 mL SWFI into the lyophilized Leukine. • DO NOT INVERT VIAL at any time. Roll the vial slowly between your hands to mix, taking care to be sure all powder is dissolved in the vial. • Reconstitution of lyophilized Leukine should take approximately 2 minutes. DO NOT SHAKE OR VORTEX.