iii. The Killing assay will employ a 120 -hour in vitro stimulation of unfractionated patient PBMCs using peptides spanning VGX-3100 antigens. Stimulated whole PBMCs or CD8+ T cells isolated after the 120 -hour incubation will be co- incubated for 60 minutes with target cells that have received a membrane stain that differentiates them from patient PBMCs/CD8+ T cells a nd have additionally been pulsed with a reagent which is activated only when the cell is actively in apoptosis (programmed cell death). At the end of this time cells will be washed and target cells identified using the membrane stain and analyzed for the presence of the active substrate as a measure of functional cytolytic degranulation and killing Employment of this assay may change pending readouts from the Lytic Granule Loading assay. Description of previous use of these assays or variants thereof can be found in Bagarazzi et al . Immunotherapy a gainst HPV16/18 [12]. The construction of this assay may vary as new information becomes available.