13 Research samples, as described in Section 5, prior to each vaccination and at each scheduled follow-up evaluation. Includes evaluation of specific lysis and cytokine release, metabolomic and bioenergetic studies (using S eahorse), intracellular FACS of cytokine production, ELISA-spot assays, and lymphocyte subset analysis may be used to evaluate the immunological correlates o f treatment. 14 As performed at baseline to determine tumor response.