9 | See next page for caption. Nature Medicine Article https://doi.org/10.1038/s41591-024-02894-yExtended Data Fig. 9 | Subcluster analysis of CD8 TEM cluster in single cell sequencing of peripheral blood T cells at week 12 post-vaccination. a, Uniform Manifold Approximation and Projection (UMAP) of single-cell transcriptomes of 3861 CD8 TEM cells from peripheral blood samples (n = 4) obtained at 12 weeks post-vaccination from 3 patients (#6, #7, and #8), colored by cluster. b , Barplot showing the number of cells occupying each CD8 TEM subcluster. c, Dot plot showing gene expression CD3E, CD8A, CCL5, CD69, NKG7, LCK, CD27, PDCD1, LAG3, TIGIT, GZMK, GZMB, GZMA, PRF1, and GNLY . across CD8 TEM subclusters. d , Heatmaps showing scaled expression of the top 10 marker genes identified for each subset. e , UMAP indicating areas of clonal expansion in 2,738 cells identified as having a TCR by paired single-cell TCR-sequencing. Colors indicate the degree of clonal expansion of each TCR clonotype. f, UMAPs indicating single cells with a TCRβ matching clonally expanded TCRs identified by bulk sequencing of pre- and post-treatment peripheral blood, divided by patient. g , CD8 TEM subcluster identities of single cells with a TCRB matching clonally expanded TCRs identified by bulk sequencing of pre- and post-treatment peripheral blood, represented as percentage of total number of single cells per patient. Nature Medicine Article https://doi.org/10.1038/s41591-024-02894-y Extended Data Fig.