Primary and secondary analyses will be summarized in a descriptive manner . Treos Bio ZRT OBERTO -101 Amendment 3 Product: PolyPEPI 1018 Colorectal Cancer Vaccine Page 66 of 81 CONFIDENTIAL 9.5.4 Exploratory Analyse s • Pearson correlation coefficients will be calculated to measure correlations. The significance of the correlations will be tested using standard method of estimating significance of correlations ( 𝑟𝑟�𝑛𝑛−2 1−𝑟𝑟2 follows the t distribution with degree of freedom n-2, where r is the Pearson correlation and n is the number of measurements) • Correlated data points will be plotted and trend lines will be obtained using perpendicular (orthogonal) regression, since both axes contain measurements and/or predicted valu es (PEPIs predicted by the PEPI T est) • The significance of the changes will be tested by non- parametric tests (Wilcoxon signed-rank test). Non- parametric test is preferred due to the small sample size and the questionable normality of the data 9.6 Determination of Sample Siz e Approximately 10 subjects will be enrolled in the study to receive three vaccinations .