When not possible or safe to obtain biopsies in the clinic, post- treatment biopsies will not be obtained. Primary tumor biopsies are limited to 10 per patient per time point; normal mucosa biopsies are limited to 2 per patient per time point; skin biopsies are limited to 1 per patient per time point. All tissue samples required for the Hamilton, Donahue and Hodge labs will be stored in the Allen lab as described later in section 5.2.2 and distributed when they are ready to perform assays. Tissue and blood samples will be assessed for correlative changes related to development of antigen-specific anti-tumor immunity, general immune activation, and potential alterations in wound healing. 5.1.1 To define immune infiltrate and localization using Multiplex immunofluorescence Multi-parameter (multi-plex) immunofluorescence using the Perkin-Elmer Opal System will be used to assess tumor samples for infiltration and location of selected immune cell subsets, including effector T cells, regulatory T cells and myeloid cells. This will be done in collaboration with the Tumor Immune Microenvironment Laboratory (TIML) in the Genitourinary Malignancies Branch, NCI managed by Dr.