5. Remove cap from vial of Test Drug (E75 acetate, 1.5 mg/mL or placebo) and wipe septum with alcohol prep pad. 6. Attach a 2 inch 21G needle to a 1.0 mL TB syringe. Leaving vial upright (do not invert), insert the 2 inch needle all the way down to the bottom of the vial and withdraw 0.8 mL into the syringe. 7. Using the needle and syringe from step #6, slowly inject the 0.8 mL of Test Drug into the reconstituted Leukine vial. 8. Keeping the vial upright, slowly roll the Leukine/Test Drug vial between your hands. Allow any foaming to dissipate before continuing.