At each site, half of each dose (0.5 mL) will be injected subcutaneously and half (0.5 mL) will be injected intradermally. The remaining 1 mL of the prepared emulsion will be discarded. The 2 mL dose of each vaccine contains 100 μg each of the 12 peptides listed in Table 1 and 200 μg of tetanus peptide (Table 2), emulsified in 1 mL Montanide ISA-51 VG adjuvant. Vaccine composition: The peptides described in Tables 1–2 will be used in the vaccines. Table 1: 12 melanoma peptides (12-MP) Allele Sequence Epitope HLA-A1 DAEKSDICTDEY Tyrosinase 240-251 * SSDYVIPIGTY Tyrosinase 146-156 EADPTGHSY MAGE-A1 161-169 EVDPIGHLY MAGE-A3 168-176 HLA-A2 YMDGTMSQV Tyrosinase 369-377  IMDQVPFSV gp100 209-217 # YLEPGPVTA gp100 280-288 GLYDGMEHL MAGE-A10 254-262 HLA-A3/A11 ALLAVGATK gp100 17-25 LIYRRRLMK gp100 614-622 SLFRAVITK MAGE-A1 96-104 ASGPGGGAPR NY-ESO-1 53-62 *(substitution of S for C at residue 244) (post-translational change of N to D at residue 371) #(209-2M, substitution of M for T at residue 210) Table 2: Tetanus toxoid-derived helper peptide **An alanine residue was added to the N-terminus to prevent cyclization. Materials Gloves (sterile) 2–3 sterile gauze packets 70% ethanol 1 – 12-MP – “12 Melanoma Peptides from MD P and CTA” [150 mcg each peptide per vial] 1 – Tet – “Peptide-tet” [300 mcg peptide per vial] 1 – Sterile water for injection 1 – Sterile field 1 - I connector 3 – Alcohol swabs, 70% 2 – 5-mL plastic syringe (Latex and sili con oil free and rubber tip free plunger) 1 – 25-gauge 5/8" needle. A 27 gauge needle may be used for injection if desired. 1 – 3-mL vial of Montanide ISA-51 VG (or larger) Allele Sequence Epitope Binds to multiple class II alleles AQYIKANSKFIGITEL p2830-844 ** NCI Protocol #: CITN-04 Version Date: 27 Jan 2016 89 2 – 19 gauge 1 ½ “needle 1 – Cap from 15 ml conical tube filled with de-ionized water 1 -- Ziploc bag 3 -- Information labels General Preparation 1. Remove 12-MP and Tet vials from the freezer. Each type of vial contai ns sterile lyophilized peptide. Thaw at room temperature (1–2 minutes).