The syringes must be used within 6 hours of mixing. Guidance for DTH Preparation from E75 Acetate Solution 1.5mg/1ml Vial To Prepare the DTH skin test, withdraw 0.07 mL of the 1.5mg/1.0mL E75 acetate solution. The DTH skin test is administered intradermally using 0.1 mL (100 μg) of the E75 acetate solution. Supplies: All DTH skin test kits will contain an active vial, which contains 1.0 mL of E75 acetate in WFI at 1.5mg/mL. Some DTH kits will contain a second vial, which is a control vial containing 1.0 mL of Water For Injection (WFI). If the DTH kit contains the WFI discard the WFI in the kit per your institution’s policy. 55 Protocol: 2014-0443 Version 2.1 Version Date: 11 April 2016 E75 acetate solution preparation: 1. Remove cap from 1.5 mg/1mL E75 acetate solution and wipe with alcohol prep pad 2. Open 1 mL syringe with needle attached and uncap the needle 3. Draw > 0.07 mL air into the syringe and inject into the E75 acetate solution vial 4. Withdraw 0.07 mL of E75 acetate solution into the syringe 5. Remove the syringe and the needle from the vial 6. Drawback syringe plunger to capture hub volume into the syringe 7. Remove and discard needle 8. Attach a new, sterile needle to the syringe containing 0.07 mL (100 ug) E75 acetate solution for intradermal administration. 56 Protocol: 2014-0443 Version 2.1 Version Date: 11 April 2016 Appendix E Leukine package insert 57 Protocol: 2014-0443 Version 2.1 Version Date: 11 April 2016 Appendix F ECOG Performance Status Criteria Score Description 0 Fully active, able to carry on all pre-disease performance without restriction 1 Restricted in physically strenuous activity but ambulatory and able to carry out work of a light or sedentary nature, e.g., light housework, office work 2 Ambulatory and capable of all self-care but unable to carry out any work activities. Up and about more than 50% of waking hours.