Study participants will not be contacted in the future for additional use of these stored blood specimens. If study participants want their blood specimens removed from storage and destroyed, they may do so by contacting the PI or research nurse at any time. Additionally, any stored blood may also be utilized to assess new generations of vaccines. For blood draws performed for immunologic assessment, 10cc are collected into a BD Vacutainer Rapid Serum tube (BD, Franklin Lakes, NJ) which contain a clot 29 Protocol: 2014-0443 Version 2.1 Version Date: 11 April 2016 activator and silicone coated interior. After centrifugation, serum will be collected, aliquoted in 1cc vials and frozen. The remaining 60cc are collected into BD Vacutainer CPT Cell Preparation tubes which contain an anticoagulant (sodium heparin or sodium citrate) with FICOLL HYPAQUE density gradient fluid and a polyester gel barrier. The density gradient fluid and the gel barrier allow for the separation of PBMC from the red blood cells by a single step centrifugation process. The PBMC fraction will be collected by centrifugation and suspended in RPMI-1640 (GIBCO, Invitrogen Corporation, Carlsbad, CA) with 10% FCS (Gemini Bio-Products, West Sacramento, CA) and antibiotics. 4.4.5 HLA-A2/A3/A24/A26 testing HLA testing will be performed at the CLIA certified human flow cytometry lab at MD Anderson. The expression of HLA-A2 by the patients is confirmed by staining PBMC with anti-HLA-A2 monoclonal antibody, (clone BB7.2), directly conjugated to phycoerythrin (PE) or fluorescein-isothiocyanate (FITC) (BD Biosciences) at 4ºC for 60 minutes. The expression of HLA-A3, A24, and A26 by the patients is confirmed by staining PBMC with the appropriate biotinylated-anti HLA-allele monoclonal antibody (One Lambda Inc.) for 45 minutes after which the cells are washed and incubated for an additional 15 minutes with streptavidin-PE. After incubation the cells are washed and analyzed on a flow cytometer. 4.4.6 Dextramer assay Fresh PBMC will be stained with aqua live/dead stain (Invitrogen) and the following antibodies: CD8 APC-H7 (BD Biosciences), CD3 PE Cy7 (BD Biosciences), E75-APC-conjugated dextramer (Immudex), and the following pacific blue conjugated lineage antibodies: CD14 (BD Biosciences), CD16 (BD Biosciences), and CD19 (Biolegend). Cells will be analyzed on a Canto flow cytometer (BD Biosciences).