10. Prior to spiking the cell bag, two RNs will perform the identification procedure. Both RNs must sign the tag on the cell bag. 11. Infuse the cells by infusion pump over 20- 30 minutes or as clinically indicated. a. Piggyback the cells into the dedicated NS line; use the backflush technique to prime the line. b. While the cells are infusing, gently agitate the bag of cells every few minutes . When the cell bag is empty, backflush NS to rinse the bag and infuse this at the same rate as the cells; rinse bag until NS runs clear. Gently agitating the bag prevents the cells from clumping in the bag. 12. Measure and record VS before and after the cell infusion, then hourly (± 15 minutes) from the end of the infusion for two hours. 13.