Re-MATC H Protocol Page 104 of 106 Version: 20180628 30. APPENDICES 30.1 Referenced Documents UFBTIP IDENTIFIER DOCUMENT TITLE BR-UFBTIP -001 Isolation of Peripheral Blood Mononuclear Cells (PBMC) Following Leukapheresis BR-UFBTIP -002 Production of Total Tumor RNA Transfected Dendritic Cell Product BR-UFBTIP -003 Procedure for the Production of Total Tumor RNA Specific T Cell Product BR-UFBTIP -004 Non-Adherent Cell Freezing Procedure SDW -UFBTIP -002 DC Vaccine Administration SDW -UFBTIP -003 ALT Vaccine Administration SDW -UFBTIP -004 Performance Status Assessment SOP-UFBTIP -100 Assay for Dendritic Cell and T cell Phenotype SOP-UFBTIP -102 RNA Extraction from total tumor sample in BSC SOP-UFBTIP -103 Small Scale cDNA Production Using Total Tumor RNA From Patient Using Reverse Transcriptase SOP-UFBTIP -104 Real Time PCR Assay to Determine the Presence of Housekeeping Genes in the cDNA From Total Tumor RNA SOP-UFBTIP -105 Large Scale cDNA Production Using Total Tumor RNA From Patient Using Reverse Transcriptase SOP-UFBTIP -106 Check of Large -Scale Reverse Transcription Reaction by PCR SOP-UFBTIP -107 Large Scale PCR Amplification of cDNA from Total Tumor RNA SOP-UFBTIP -108 Small Scale Amplified DNA Clean Up Using Qiaquick Purification Kit SOP-UFBTIP -109 Small Scale IVT Reaction From Small Scale DNA Clean Up of the Large scale PCR Amplification using T7 mMe ssage mMachine Kit SOP-UFBTIP -110 Clean Up of Small Scale IVT RNA with RNeasy kit from Qiagen SOP-UFBTIP -111 cDNA Synthesis From Small Scale IVT RNA SOP-UFBTIP -112 Real Time PCR Assay to Determine the Presence of Housekeeping Genes in the Small Scale IV T RNA SOP-UFBTIP -113 Large Scale Clean Up of Large Scale PCR Amplification using Qiagen PCR Purification Kit SOP-UFBTIP -114 Small Scale IVT Reaction From Small Scale DNA Clean Up of the Large scale PCR Amplification using T7 mMessage mMachine Kit SOP-UFBTIP -115 Clean Up of Small Scale IVT RNA with RNeasy kit from Qiagen SOP-UFBTIP -116 Procurement and Transport of the Tissue Sample From the Surgical Suite to the Laboratory SOP-UFBTIP -119 RNA Release Criteria SOP-UFBTIP -120 External Tissue Collect ion, Snap Frozen SOP-UFBTIP -121 Leukapheresis Product Transport and Receipt Procedures SOP-UFBTIP -122 Preparation of RNA Transfected DC Product for QC Testing SOP-UFBTIP -123 Preparation of RNA Stimulated T Cell Product for QC Testing SOP-UFBTIP -124 Procedure for DC Vaccine Preparation SOP-UFBTIP -125 Procedure for Preparation of TTRNA -ALT for Adoptive Transfer Re-MATC H Protocol Page 105 of 106 Version: 20180628 UFBTIP IDENTIFIER DOCUMENT TITLE SOP-UFBTIP -126 Isolation and Cryopreservation of Peripheral Blood Mononuclear Cells (PBMC) and Plasma From Whole Blood for Immune Monitoring SOP-UFBTIP -127 Leukapheresis Transport SOP-UFBTIP -128 Administration of DC Vaccine SOP-UFBTIP -129 Vaccine Transport SOP-UFBTIP -130 External Tissue Collection, fresh tissue SOP-UFBTIP -131 Leukapheresis for Peripheral Blood Mononuclear Cells (PBMCs) SOP-UFBTIP -132 Administration of ALT (adoptive lymphocyte transfer) Re-MATC H Protocol Page 106 of 106 Version: 20180628 31. ADDENDUM A 31.1 Single Patient -Specific Protocol (UF Site Only)