5. Remove cap from vial of Test Drug (E75 acetate, 1.5 mg/mL or placebo) and wipe septum with alcohol prep pad. 6. Attach a 2 inch 21G needle to a 1.0 mL TB syringe. Leaving vial upright (do not invert), insert the 2 inch needle all the way down to the bottom of the vial and withdraw 0.8 mL into the syringe.