The absence of a male heir to continue the dynasty was the cause of constant conflict with her husband, who preferred the company of his mistress Bianca Cappello, who gave birth to a son, Antonio, in 1576.
Absența unui moștenitor de sex masculin care să continue dinastia, a fost cauza unui conflict permanent cu soțul ei, care prefera compania amantei sale Bianca, care i-a dăruit un fiu, Antonio, în 1576.