Mary was already unpopular, and her marriage on 15 May 1567 to James Hepburn, 4th Earl of Bothwell, who was widely suspected of murdering Darnley, heightened widespread bad feeling towards her.
Maria era deja nepopulară și căsătoria ei la 15 mai 1567 cu James Hepburn, al 4-lea conte de Bothwell, care era suspectat că era implicat în uciderea lui Darnley, a sporit la nepopularitatea ei.