He also became the main witness against Queen Mary, though her involvement in the murder, thought to have been carried out by her later husband, Lord Bothwell, is controversial.
Također je postao glavnim svjedokom protiv kraljice Marije, iako je njezina umještanost u ubojstvo, navodno izvršena preko njezina kasnijega muža Jamesa Hepburna, 4. grofa od Bothwella, ostaje kontroverznom.