In 2015, the team led by Ned Budisa reported the successful completion of a long-term evolution experiment that resulted in full, proteome-wide substitution of all 20,899 tryptophan residues with thienopyrrole-alanine in the genetic code of the bacterium Escherichia coli.
Godine 2015, Budišina grupa je uspješno provela dugoročni eksperiment mikrobne laboratorijske evolucije koji je rezultirao potpunom, proteomskom zamjenom svih 20.899 triptofanskih ostataka s tienopirol-alaninom u genetičkom kodu (uputi) bakterije Escherichia coli.