The series takes place behind the scenes of a live sketch comedy show (also called Studio 60 on the Sunset Strip or Studio 60) on the fictional television network NBS (National Broadcasting System), whose format is similar to that of NBC's Saturday Night Live.
Radnja serije odvija se iza kulisa izmišljenog humorističnog showa (također znanog i pod imenom "Studio 60 na Sunset Stripu" ili samo "Studio 60") na izmišljenoj televizijskoj mreži NBS (National Broadcasting System), a čiji je format sličan onome koji ima NBC-jev popularni show Saturday Night Live.