When enzymes bind multiple substrates, such as dihydrofolate reductase (shown right), enzyme kinetics can also show the sequence in which these substrates bind and the sequence in which products are released.
Når enzymer binder flere substrater, såsom dihydrofolatreduktase (vist til højre), kan enzymkinetik også vise den sekvens hvormed substraterne binder og den sekvens i hvilken produkter afgives.