His wife, Eleanor of Toledo, died in 1562, along with four of his children due to a plague epidemic in Florence.
Η σύζυγός του, Ελεονόρα του Τολέδο, πέθανε το 1562, μαζί με τα τέσσερα παιδιά του, εξαιτίας μιας επιδημίας πανώλης στη Φλωρεντία.