Libraries of DNA oligonucleotides manufactured by an In Vitro selection protocol were characterized for their non-crosshybridizing properties. Cloning and sequencing after several iterations of the protocol showed that the sequences, in general, became more non-crosshybridizing. Gel electrophoresis of protocol product, also, indicated non-crosshybridization, and showed evolution in the population of molecules under the non-crosshybridization selection pressure. Melting curves of protocol product also indicated non-crosshybridization when compared to control samples. Thus, it appears that the protocol does select populations of non-crosshybridizing sequences.
['Junghuei Chen', 'Russell J. Deaton', 'Max H. Garzon', 'Jin Woo Kim', 'David Harlan Wood', 'Hong Bi', 'Dylan Carpenter', 'Yuzhen Wang']
Characterization of non-crosshybridizing DNA oligonucleotides manufactured In Vitro