Motivation: We present a pipeline for the pre-processing, quality assessment, read distribution and methylation estimation for methylated DNA immunoprecipitation (MeDIP)-sequence datasets. This is the first MeDIP-seq-specific analytic pipeline that starts at the output of the sequencers. This pipeline will reduce the data analysis load on staff and allows the easy and straightforward analysis of sequencing data for DNA methylation. The pipeline integrates customized scripting and several existing tools, which can deal with both paired and single end data. Availability: The package and extensive documentation, and comparison to public data is available at http://life.tongji.edu.cn/ meqa/ Contact: jhuang@cephb.fr
['Jia Huang', 'Valérie Renault', 'Jennifer Sengenès', 'Nizar Touleimat', 'S. Michel', 'Mark Lathrop', 'Jörg Tost']
MeQA: a pipeline for MeDIP-seq data quality assessment and analysis