The authors developed a method for mapping the change in cerebral glucose utilization at two different physiological states using [/sup 18/F]fluorodeoxyglucose (FDC) double infection and the constrained weighted-integration method. The authors studied young normal subjects without (baseline-baseline group, n=5) and with (baseline-stimulation group, n=5) vibrotactile stimulation of the fingertips of the right hand. Dynamic scans were performed using positron emission tomography (PET) following an initial dose (the first session, 0-30 min) and an additional dose (the second session, 30-60 min). The parametric images of the net clearance of FDG from blood to brain (K*), unidirectional blood-to-brain clearance (K/sub 1/*), and cerebral metabolic rate of glucose (CMR/sub glc/) of the two sessions were generated. The averaged subtraction (second minus first session) and t-statistic images were generated, which were rendered into Talairach's stereotaxic coordinates and merged with the averaged magnetic resonance imaging (MRI) image. In the baseline-baseline group, regional K*, K/sub 1/* and CMR/sub glc/ in the first and second sessions were strongly correlated (r/sup 2/=0.953, 0.935, and 0.951, respectively, n=330). In the baseline-stimulation group, significant increases in these estimates were obtained in the contralateral primary somatosensory cortex (SI) (from 3.43/spl plusmn/0.78 to 4.02/spl plusmn/1.01 ml/100 g/min for K*, 7.85/spl plusmn/1.88 to 9.09/spl plusmn/1.71 ml/100 g/min for K/sub 1/*, and 280/spl plusmn/5.9 to 32.3/spl plusmn/5.5 /spl mu/mol/100 g/min for CMR/sub glc/), while there were no significant changes in the ipsilateral SI (from 3.45/spl plusmn/0.83 to 359/spl plusmn/0.72 ml/100 g/min for K*, 8.17/spl plusmn/2.33 to 837/spl plusmn/1.75 ml/100 g/min for K/sub 1/* and 29.5/spl plusmn/8.1 to 29.1/spl plusmn/8.2 /spl mu/mol/100 g/mln for CMR/sub glc/), Significant increases in K* and CMR/sub glc/ in the contralateral SI were clearly demonstrated in the t-statistic image. In conclusion, the proposed method allows mapping of changes in cerebral glucose utilization during physiological stimulation of the brain, and will be useful for studying the relationship between neural activity and regional cerebral glucose metabolism.
['Kenya Murase', 'Hiroto Kuwabara', 'Yoshifumi Yasuhara', 'Alan C. Evans', 'Albert Gjedde']
Mapping of change in cerebral glucose utilization using fluorine-18 fluorodeoxyglucose double injection and the constrained weighted-integration method