Together with our efforts of developing low-cost and high throughput DNA analysis chips based on microfluidics technologies, an automated and integrated system has been developed for real-time polymerase chain reaction (PCR) analysis based on the microfluidic PCR array chips. In order to yield rapid and stable thermal cycling in liquid sample contained in biochip wells, efforts have been made on optimizing thermal cycling performance through PID control. Rapid and homogenous PCR thermal cycling has been achieved with the system. In this paper, we present the system instrumentation and its thermal cycling control.
['Qinghui Wang', 'Yin Tan', 'Haiqing Gong']
AN INTEGRATED SYSTEM FOR REAL-TIME PCR ANALYSIS BASED ON MICROFLUIDIC BIOCHIP