Recently, there are a lot of approaches to form tissue engineered organs. In this presentation, we introduce the approaches based on 2 concepts of scaffold-free tissue formation and decellularized tissue models. Organ models with/without scaffolds showed good mechanical and biological properties in cartilage and uterus tissues. In scaffold-free models, mechanical stress loading was available to improve the properties of cartilage. The cartilage tissue which constituted of chondrocytes and ECM produced by inoculated dedifferentiated chondrocytes to a high porous simple mold has arbitrary shapes, and did not need any biodegradable scaffold to control the shape. We also showed the transplanted the constructs for osteochondral defects using a rabbit model has good biocompatibility in vivo. In decellularized models of uterus, it was easy to re-construct the tissue in vivo. The subsequent pregnancy test after 30 days transplantation revealed successful pregnancy for both the SDS and HHP groups. These findings indicate that the decellularized matrix from the uterine tissue can be a potential scaffold for uterine regeneration.
['Katsuko Furukawa']
Tissue engineering approaches with/without scaffold