In this study metabolic footprinting was applied to evaluate the metabolic consequences of protein overproduction at slow growth conditions (μ = 0.1 h -1 ). The extracellular metabolites detected by gas chromatography-mass spectrometry char- acterized the metabolic footprints before and after the induction of the recombinant protein production (i.e. pre- and post-induction phases). Metabolic footprinting en- abled the discrimination between the two growth phases and exposed significant al- terations in the extracellular milieu during the recombinant process.
['Sónia Carneiro', 'Silas G. Villas-Boas', 'I. Rocha', 'E. C. Ferreira']
Applying a Metabolic Footprinting Approach to Characterize the Impact of the Recombinant Protein Production in Escherichia coli