This paper reported a cell system that fabricates hepatic lobule-like microtissue (HLLM) based on alginate-Calcium electrodeposition and cell microcapsule method. The property of the fabricated HLLM includes: 1) With a shape similar to our native hepatic lobule tissue. 2) High cell dense (∼10 8 ). 3) Easily be moved and retrieved for further 3D assembly. Therefore, we proposed a repetitive single-step manipulation method to assemble these single HLLM into 3D multilayer hepatic lobule model. The pick-up success rate was approximating 90%. The assembly speed was about 0.4 microtissue/min and the cell viability was higher than 80%. Unlike other assembly methods, our method is sufficiently repeatable and easy to assemble many single micro modules. The fabricated 3D hepatic lobule model with high cell density is essential for tissue engineering applications.
['Zeyang Liu', 'Masaru Takeuchi', 'Masahiro Nakajima', 'Toshio Fukuda', 'Yasuhisa Hasegawa', 'Qiang Huang']
Assembly of hepatic lobule-like microtissue with repetitive single-step contact manipulation