Background#R##N#Zinc Finger Nucleases (ZFNs) are man-made restriction enzymes useful for manipulating genomes by cleaving target DNA sequences. ZFNs allow therapeutic gene correction or creation of genetically modified model organisms. ZFN specificity is not absolute; therefore, it is essential to select ZFN target sites without similar genomic off-target sites. It is important to assay for off-target cleavage events at sites similar to the target sequence.
['Thomas J Cradick', 'Giovanna Ambrosini', 'Christian Iseli', 'Philipp Bucher', 'Anton P. McCaffrey']
ZFN-site searches genomes for zinc finger nuclease target sites and off-target sites.