Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely: The analysis on codon usage bias of UL26.5 gene of duck enteritis virus (DEV) may provide a basis for understanding the evolution and pathogenesis of DEV and for selecting appropriate host expression systems to improve the expression of target genes in vivo and in vitro. In this study, the synonymous codon usage in the UL26.5 gene of DEV and 27 reference herpesviruses have been investigated . The results reveals that the synonymous codons with A and T at the third codon positon have widely usage in the codon of UL26.5 gene of DEV. G + C compositional constraint is the main factor that determines the codon usage bias in UL26.5 gene. The phylogentic analysis suggested that DEV was evolutionarily closer to fowl herpesviruses which further clustered into Alphaherpesvirinae. There re both 21 codons showing distinct usage differences beten DEV with Escherichia coli and yeast, 25 beten DEV and Homo sapiens, 26 beten DEV and adenovirus. Therefore the yeast and E. coli expression system may be suitable for the expression of DEV UL26.5 gene if some codons could be optimized.
['Yao Zhang', 'Anchun Cheng', 'Mingshu Wang', 'Dekang Zhu', 'Renyong Jia', 'Fei Liu', 'Xiaoyue Chen']
I am writing a research paper on a idea Analysis of Synonymous Codon Usage in the UL26.5 Gene of Duck Enteritis Virus
[{"from": "human", "value": "I am writing a research paper on a idea Analysis of Synonymous Codon Usage in the UL26.5 Gene of Duck Enteritis Virus"}, {"from": "gpt", "value": "Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely: The analysis on codon usage bias of UL26.5 gene of duck enteritis virus (DEV) may provide a basis for understanding the evolution and pathogenesis of DEV and for selecting appropriate host expression systems to improve the expression of target genes in vivo and in vitro. In this study, the synonymous codon usage in the UL26.5 gene of DEV and 27 reference herpesviruses have been investigated . The results reveals that the synonymous codons with A and T at the third codon positon have widely usage in the codon of UL26.5 gene of DEV. G + C compositional constraint is the main factor that determines the codon usage bias in UL26.5 gene. The phylogentic analysis suggested that DEV was evolutionarily closer to fowl herpesviruses which further clustered into Alphaherpesvirinae. There re both 21 codons showing distinct usage differences beten DEV with Escherichia coli and yeast, 25 beten DEV and Homo sapiens, 26 beten DEV and adenovirus. Therefore the yeast and E. coli expression system may be suitable for the expression of DEV UL26.5 gene if some codons could be optimized."}]