Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely: Summary: SEPON, Selection and Evaluation Pipeline for OligoNucleotide generates n-mer oligonucleotide sequences from expressed sequence tags of non-annotated genomes for microarray gene-expression profiling. A non-target melting temperature (Tm) algorithm will reduce cross-hybridization by estimating Tm of oligonucleotide hybridization to non-specific targets (non-target Tm) and discard oligonucleotides with non-target Tm estimate above user-defined threshold. SEPON allows user-defined filtering, predicts exon location, assigns penalty based on 3' distance, GC content, secondary structure Tm and non-target Tm and ranks oligonucleotides for optimal selection.#R##N##R##N#Availability: The program is freely available for non-commercial and academic use under the GNU General Public License and is obtained upon request to authors.#R##N##R##N#Supplementary information: http://www.agrsci.dk/hag/sepon/
['Henrik Hornshøj', 'Henrik Stengaard', 'Frank Panitz', 'Christian Bendixen']
I am writing a research paper on a idea SEPON, a Selection and Evaluation Pipeline for OligoNucleotides based on ESTs with a non-target Tm algorithm for reducing cross-hybridization in microarray gene expression experiments
[{"from": "human", "value": "I am writing a research paper on a idea SEPON, a Selection and Evaluation Pipeline for OligoNucleotides based on ESTs with a non-target Tm algorithm for reducing cross-hybridization in microarray gene expression experiments"}, {"from": "gpt", "value": "Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely: Summary: SEPON, Selection and Evaluation Pipeline for OligoNucleotide generates n-mer oligonucleotide sequences from expressed sequence tags of non-annotated genomes for microarray gene-expression profiling. A non-target melting temperature (Tm) algorithm will reduce cross-hybridization by estimating Tm of oligonucleotide hybridization to non-specific targets (non-target Tm) and discard oligonucleotides with non-target Tm estimate above user-defined threshold. SEPON allows user-defined filtering, predicts exon location, assigns penalty based on 3' distance, GC content, secondary structure Tm and non-target Tm and ranks oligonucleotides for optimal selection.#R##N##R##N#Availability: The program is freely available for non-commercial and academic use under the GNU General Public License and is obtained upon request to authors.#R##N##R##N#Supplementary information: http://www.agrsci.dk/hag/sepon/"}]