Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely: The impact of pulse repetition rate (PRR) in modulating electroporation (EP) induced by nanosecond pulsed electric fields (nsPEFs) in mammalian cells was approached here by performing both biological and numerical analysis. Plasma membrane permeabilization and viability of Jurkat cells re analyzed after exposure to 500, 1.3 MV/m, 40 ns PEFs with variable PRR (2–30 Hz). A finite-element model was used to investigate EP dynamics in a single cell under the same pulsing conditions, by looking at the time course of transmembrane voltage and pore density on the ns time scale. The biological observations shod an increased EP and reduced viability of the exposed cells at lor PRR in the considered range. The numerical analysis resulted in different dynamics of plasma membrane response when ns pulses re delivered with different PRR, consistently with a phenomenon of electrodesensitization recently hypothesized by another research group.
['Patrizia Lamberti', 'Stefania Romeo', 'Anna Sannino', 'Luigi Zeni', 'Olga Zeni']
I am writing a research paper on a idea The Role of Pulse Repetition Rate in nsPEF-Induced Electroporation: A Biological and Numerical Investigation
[{"from": "human", "value": "I am writing a research paper on a idea The Role of Pulse Repetition Rate in nsPEF-Induced Electroporation: A Biological and Numerical Investigation"}, {"from": "gpt", "value": "Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely: The impact of pulse repetition rate (PRR) in modulating electroporation (EP) induced by nanosecond pulsed electric fields (nsPEFs) in mammalian cells was approached here by performing both biological and numerical analysis. Plasma membrane permeabilization and viability of Jurkat cells re analyzed after exposure to 500, 1.3 MV/m, 40\u00a0ns PEFs with variable PRR (2\u201330\u00a0Hz). A finite-element model was used to investigate EP dynamics in a single cell under the same pulsing conditions, by looking at the time course of transmembrane voltage and pore density on the ns time scale. The biological observations shod an increased EP and reduced viability of the exposed cells at lor PRR in the considered range. The numerical analysis resulted in different dynamics of plasma membrane response when ns pulses re delivered with different PRR, consistently with a phenomenon of electrodesensitization recently hypothesized by another research group."}]