Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely: Off-target effects are one of the most serious problems in RNA interference (RNAi). Here,  present dsCheck (http://dsCheck.RNAi.jp/), b-based online software for estimating off-target effects caused by the long double-stranded RNA (dsRNA) used in RNAi studies. In the biochemical process of RNAi, the long dsRNA is cleaved by Dicer into shortinterfering RNA (siRNA) cocktails. The software simulates this process and investigates individual 19 nt substrings of the long dsRNA. Subsequently, the software promptly enumerates a list of potential off-target gene candidates based on the order of off-target effects using its novel algorithm, which significantly improves both the efficiency and the sensitivity of the homology search. The bsite not only provides a rigorous off-target search to verify previously designed dsRNA sequences but also presents ‘offtarget minimized’ dsRNA design, which is essential for reliable experiments in RNAi-based functional genomics.
['Yuki Naito', 'Tomoyuki Yamada', 'Takahiro Matsumiya', 'Kumiko Ui-Tei', 'Kaoru Saigo', 'Shinichi Morishita']
I am writing a research paper on a idea dsCheck: highly sensitive off-target search software for double-stranded RNA-mediated RNA interference
[{"from": "human", "value": "I am writing a research paper on a idea dsCheck: highly sensitive off-target search software for double-stranded RNA-mediated RNA interference"}, {"from": "gpt", "value": "Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely: Off-target effects are one of the most serious problems in RNA interference (RNAi). Here,  present dsCheck (http://dsCheck.RNAi.jp/), b-based online software for estimating off-target effects caused by the long double-stranded RNA (dsRNA) used in RNAi studies. In the biochemical process of RNAi, the long dsRNA is cleaved by Dicer into shortinterfering RNA (siRNA) cocktails. The software simulates this process and investigates individual 19 nt substrings of the long dsRNA. Subsequently, the software promptly enumerates a list of potential off-target gene candidates based on the order of off-target effects using its novel algorithm, which significantly improves both the efficiency and the sensitivity of the homology search. The bsite not only provides a rigorous off-target search to verify previously designed dsRNA sequences but also presents \u2018offtarget minimized\u2019 dsRNA design, which is essential for reliable experiments in RNAi-based functional genomics."}]