Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely: Motivation: Zinc-binding proteins are the most abundant metallo-proteins in Protein Data Bank (PDB). Accurate prediction of zinc-binding sites in proteins of unknown function may provide important clues for the inference of protein function. As zinc binding is often associated with characteristic 3D arrangements of zinc ligand residues, its prediction may benefit from using not only the sequence information but also the structure information of proteins.#R##N##R##N#Results: In this work,  present a structure-based method, TEMSP (3D TEmplate-based Metal Site Prediction), to predict zinc-binding sites. TEMSP significantly improves over previously reported best methods in predicting as many as possible true ligand residues for zinc with minimum overpredictions: if only those results in which all zinc ligand residues have been correctly predicted are defined as true positives, our method improves sensitivity from less than 30% to above 60%, and selectivity from around 25% to 80%. These results are for predictions based on apo state structures. In addition, the method can predict the zinc-bound local structures reliably, generating predictions useful for function inference.  applied TEMSP to 1888 protein structures of the ‘Unknown Function’ class in the PDB database. A number of zinc-binding sites have been discovered de novo, i.e. based solely on the protein structures. Using the predicted local structures of these sites, possible functional roles re analyzed.#R##N##R##N#Availability: TEMSP is freely available from http://netalign.ustc.edu.cn/temsp/.#R##N##R##N#Contact:hyliu@ustc.edu.cn; mkteng@ustc.edu.cn#R##N##R##N#Supplementary information:Supplementary data are available at Bioinformatics online.
['Wei Zhao', 'Meng Xu', 'Zhi Liang', 'Bo Ding', 'Liwen Niu', 'Haiyan Liu', 'Maikun Teng']
I am writing a research paper on a idea Structure-based de novo prediction of zinc-binding sites in proteins of unknown function
[{"from": "human", "value": "I am writing a research paper on a idea Structure-based de novo prediction of zinc-binding sites in proteins of unknown function"}, {"from": "gpt", "value": "Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely: Motivation: Zinc-binding proteins are the most abundant metallo-proteins in Protein Data Bank (PDB). Accurate prediction of zinc-binding sites in proteins of unknown function may provide important clues for the inference of protein function. As zinc binding is often associated with characteristic 3D arrangements of zinc ligand residues, its prediction may benefit from using not only the sequence information but also the structure information of proteins.#R##N##R##N#Results: In this work,  present a structure-based method, TEMSP (3D TEmplate-based Metal Site Prediction), to predict zinc-binding sites. TEMSP significantly improves over previously reported best methods in predicting as many as possible true ligand residues for zinc with minimum overpredictions: if only those results in which all zinc ligand residues have been correctly predicted are defined as true positives, our method improves sensitivity from less than 30% to above 60%, and selectivity from around 25% to 80%. These results are for predictions based on apo state structures. In addition, the method can predict the zinc-bound local structures reliably, generating predictions useful for function inference.  applied TEMSP to 1888 protein structures of the \u2018Unknown Function\u2019 class in the PDB database. A number of zinc-binding sites have been discovered de novo, i.e. based solely on the protein structures. Using the predicted local structures of these sites, possible functional roles re analyzed.#R##N##R##N#Availability: TEMSP is freely available from http://netalign.ustc.edu.cn/temsp/.#R##N##R##N#Contact:hyliu@ustc.edu.cn; mkteng@ustc.edu.cn#R##N##R##N#Supplementary information:Supplementary data are available at Bioinformatics online."}]