Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely:  compared quantitative experimental results on the diffusion of /sup 35/S-labeled phosphorothioate oligonucleotide (PS-ODN) after intraparenchymal infusion in rat brain, with the distributions predicted by Fick's second law of diffusion. Fischer 344 rats undernt identical intracerebral infusions of /sup 35/S-PS-ODN. After 0, 5, 11, 23, and 47 h, groups of animals re sacrificed and sequential brain cryosections subjected to autoradiography. The resulting experimental data re compared to the predicted distributions, for estimation of the apparent free diffusion coefficient, D*. Volumes of distribution and total content of /sup 35/S-PS-ODN in the parenchyma re also computed, to monitor loss of total material. The values for D* re within the expected range for the 21-mer PS-ODN used, but a progressive decrease in D* over time was noted. The model requires D* to remain constant and, thus, does not adequately explain the spread of /sup 35/S-PS-ODN following infusion. The progressive slowing of spread over time suggests that at later time points, /sup 35/S-PS-ODN may be fixed by tissue binding or cellular uptake in the brain. Loss of material via vascular and CSF clearance may also contribute to the lack of fit. Our results highlight issues to be addressed in the modeling and experimental design of the intraparenchymal infusion process.
['Peter J. Haar', 'John E. Stewart', 'G. T. Gillies', 'Sujit S. Prabhu', 'William C. Broaddus']
I am writing a research paper on a idea Quantitative three-dimensional analysis and diffusion modeling of oligonucleotide concentrations after direct intraparenchymal brain infusion
[{"from": "human", "value": "I am writing a research paper on a idea Quantitative three-dimensional analysis and diffusion modeling of oligonucleotide concentrations after direct intraparenchymal brain infusion"}, {"from": "gpt", "value": "Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely:  compared quantitative experimental results on the diffusion of /sup 35/S-labeled phosphorothioate oligonucleotide (PS-ODN) after intraparenchymal infusion in rat brain, with the distributions predicted by Fick's second law of diffusion. Fischer 344 rats undernt identical intracerebral infusions of /sup 35/S-PS-ODN. After 0, 5, 11, 23, and 47 h, groups of animals re sacrificed and sequential brain cryosections subjected to autoradiography. The resulting experimental data re compared to the predicted distributions, for estimation of the apparent free diffusion coefficient, D*. Volumes of distribution and total content of /sup 35/S-PS-ODN in the parenchyma re also computed, to monitor loss of total material. The values for D* re within the expected range for the 21-mer PS-ODN used, but a progressive decrease in D* over time was noted. The model requires D* to remain constant and, thus, does not adequately explain the spread of /sup 35/S-PS-ODN following infusion. The progressive slowing of spread over time suggests that at later time points, /sup 35/S-PS-ODN may be fixed by tissue binding or cellular uptake in the brain. Loss of material via vascular and CSF clearance may also contribute to the lack of fit. Our results highlight issues to be addressed in the modeling and experimental design of the intraparenchymal infusion process."}]