Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely:  report herein on the application of Raman spectroscopy to the rapid quantitative analysis of polyhydroxyalkanoates (PHAs), biodegradable polyesters accumulated by various bacteria. This theme was exemplified for quantitative detection of the most common member of PHAs, poly(3-hydroxybutyrate) (PHB) in Cupriavidus necator H16.  have identified the relevant spectral region (800–1800 cm−1) incorporating the Raman emission lines exploited for the calibration of PHB (PHB line at 1736 cm−1) and for the selection of the two internal standards (DNA at 786 cm−1 and Amide I at 1662 cm−1). In order to obtain quantitative data for calibration of intracellular content of PHB in bacterial cells reference samples containing PHB amounts—determined by gas chromatography—from 12% to 90% (w/w) re used. Consequently, analytical results based on this calibration can be used for fast and reliable determination of intracellular PHB content during biotechnological production of PHB since the whole procedure—from bacteria sampling, centrifugation, and sample preparation to Raman analysis—can take about 12 min. In contrast, gas chromatography analysis takes approximately 8 h.
['Ota Samek', 'Stanislav Obruca', 'Martin Šiler', 'Petr Sedláček', 'Pavla Benesova', 'Dan Kucera', 'Ivana Márová', 'Jan Ježek', 'S. Bernatova', 'Pavel Zemánek']
I am writing a research paper on a idea Quantitative Raman Spectroscopy Analysis of Polyhydroxyalkanoates Produced by Cupriavidus necator H16
[{"from": "human", "value": "I am writing a research paper on a idea Quantitative Raman Spectroscopy Analysis of Polyhydroxyalkanoates Produced by Cupriavidus necator H16"}, {"from": "gpt", "value": "Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely:  report herein on the application of Raman spectroscopy to the rapid quantitative analysis of polyhydroxyalkanoates (PHAs), biodegradable polyesters accumulated by various bacteria. This theme was exemplified for quantitative detection of the most common member of PHAs, poly(3-hydroxybutyrate) (PHB) in Cupriavidus necator H16.  have identified the relevant spectral region (800\u20131800 cm\u22121) incorporating the Raman emission lines exploited for the calibration of PHB (PHB line at 1736 cm\u22121) and for the selection of the two internal standards (DNA at 786 cm\u22121 and Amide I at 1662 cm\u22121). In order to obtain quantitative data for calibration of intracellular content of PHB in bacterial cells reference samples containing PHB amounts\u2014determined by gas chromatography\u2014from 12% to 90% (w/w) re used. Consequently, analytical results based on this calibration can be used for fast and reliable determination of intracellular PHB content during biotechnological production of PHB since the whole procedure\u2014from bacteria sampling, centrifugation, and sample preparation to Raman analysis\u2014can take about 12 min. In contrast, gas chromatography analysis takes approximately 8 h."}]