Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely: Abstract As a result of a high rate of mutations and recombination events, an RNA-virus exists as a heterogeneous “swarm” of mutant variants. The long read length offered by single-molecule sequencing technologies allows each mutant variant to be sequenced in a single pass. Hover, high error rate limits the ability to reconstruct heterogeneous viral population composed of rare, related mutant variants. In this article,  present two single-nucleotide variants (2SNV), a method able to tolerate the high error rate of the single-molecule protocol and reconstruct mutant variants. 2SNV uses linkage beten single-nucleotide variations to efficiently distinguish them from read errors. To benchmark the sensitivity of 2SNV,  performed a single-molecule sequencing experiment on a sample containing a titrated level of known viral mutant variants. Our method is able to accurately reconstruct clone with frequency of 0.2% and distinguish clones that differed in only two nucleotides distantly located on the genome...
['Alexander Artyomenko', 'Nicholas C. Wu', 'Serghei Mangul', 'Eleazar Eskin', 'Ren Sun', 'Alexander Zelikovsky']
I am writing a research paper on a idea Long Single-Molecule Reads Can Resolve the Complexity of the Influenza Virus Composed of Rare, Closely Related Mutant Variants.
[{"from": "human", "value": "I am writing a research paper on a idea Long Single-Molecule Reads Can Resolve the Complexity of the Influenza Virus Composed of Rare, Closely Related Mutant Variants."}, {"from": "gpt", "value": "Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely: Abstract As a result of a high rate of mutations and recombination events, an RNA-virus exists as a heterogeneous \u201cswarm\u201d of mutant variants. The long read length offered by single-molecule sequencing technologies allows each mutant variant to be sequenced in a single pass. Hover, high error rate limits the ability to reconstruct heterogeneous viral population composed of rare, related mutant variants. In this article,  present two single-nucleotide variants (2SNV), a method able to tolerate the high error rate of the single-molecule protocol and reconstruct mutant variants. 2SNV uses linkage beten single-nucleotide variations to efficiently distinguish them from read errors. To benchmark the sensitivity of 2SNV,  performed a single-molecule sequencing experiment on a sample containing a titrated level of known viral mutant variants. Our method is able to accurately reconstruct clone with frequency of 0.2% and distinguish clones that differed in only two nucleotides distantly located on the genome..."}]