Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely: A miniaturized microfluidic device was developed to facilitate electromanipulation of bacterial respiratory pathogens. The device comprises a microchip with circular aluminum electrodes patterned on glass, which is housed in a microfluidic system fabricated utilizing polydimethylsiloxane. The device provides sample preparation capability by exploiting positive dielectrophoresis (DEP) in conjunction with pulsed voltage for manipulation and disruption of  Bordetella   pertussis  bacterial cells. Positive DEP capture of  B.   pertussis  was successfully demonstrated utilizing 10 V rms  and 1 MHz ac fields. Application of dc pulses (300 V amplitude and 50 mu s pulsewidth applied 1 s apart) across the aluminum electrodes resulted in electrodisruption and lysis of  B.   pertussis  bacterial cells. Real-time polymerase chain reaction, a 2 3  factorial experimental design and transmission electron microscopy re used to evaluate bacterial cell manipulation and factors affecting bacterial cell disruption. The main factors affecting bacterial cell disruption re electric field strength, the electrical conductivity of the cell suspension sample, and the combined effect of number of pulses and sample conductivity. The bacterial deoxyribonucleic acid target remained undamaged as a result of DEP and cell lysis experimentation. Our findings suggest that a simple miniaturized microfluidic device can achieve important steps in sample preparation on-chip involving respiratory bacterial pathogens.
['C. de la Rosa', 'P.A. Tilley', 'Julie D. Fox', 'Karan V. I. S. Kaler']
I am writing a research paper on a idea Microfluidic Device for Dielectrophoresis Manipulation and Electrodisruption of Respiratory Pathogen Bordetella pertussis
[{"from": "human", "value": "I am writing a research paper on a idea Microfluidic Device for Dielectrophoresis Manipulation and Electrodisruption of Respiratory Pathogen Bordetella pertussis"}, {"from": "gpt", "value": "Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely: A miniaturized microfluidic device was developed to facilitate electromanipulation of bacterial respiratory pathogens. The device comprises a microchip with circular aluminum electrodes patterned on glass, which is housed in a microfluidic system fabricated utilizing polydimethylsiloxane. The device provides sample preparation capability by exploiting positive dielectrophoresis (DEP) in conjunction with pulsed voltage for manipulation and disruption of  Bordetella   pertussis  bacterial cells. Positive DEP capture of  B.   pertussis  was successfully demonstrated utilizing 10 V rms  and 1 MHz ac fields. Application of dc pulses (300 V amplitude and 50 mu s pulsewidth applied 1 s apart) across the aluminum electrodes resulted in electrodisruption and lysis of  B.   pertussis  bacterial cells. Real-time polymerase chain reaction, a 2 3  factorial experimental design and transmission electron microscopy re used to evaluate bacterial cell manipulation and factors affecting bacterial cell disruption. The main factors affecting bacterial cell disruption re electric field strength, the electrical conductivity of the cell suspension sample, and the combined effect of number of pulses and sample conductivity. The bacterial deoxyribonucleic acid target remained undamaged as a result of DEP and cell lysis experimentation. Our findings suggest that a simple miniaturized microfluidic device can achieve important steps in sample preparation on-chip involving respiratory bacterial pathogens."}]