Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely: This current study presents a new miniature, integrated system capable of rapid extraction of genomic DNA (gDNA) from saliva samples. Three major components of the DNA extraction chip, including six symmetrical, normally-closed microvalves, a sample transport/mixing unit and a waste unit are integrated in this device. The microfluidic DNA extraction chip was made of CNC machining and PDMS casting processes to integrate a liquid channel layer, an air chamber layer and a glass layer on a single chip. Liquid samples can be transported and mixed by the suction-type sample transport/mixing unit by using micropumps, micromixers and normally-closed microvalves. Experimental results shod the pumping rate can be as high as 400 µL/min at an applied pressure of −70 KPa for an air chamber with a depth of 1.5 mm. A mixing efficiency as high as 96.5% can be achieved within 3 sec. Experimental data also shod that gDNA with an average concentration of 45 ± 3 ng/µL and an optical intensity (OD) value (260/280) of 1.5 ± 0.2 from ten measurements can be successfully achieved within 25 minutes. Consequently, the proposed miniature system can provide a porful platform for automatic, rapid DNA extraction.
['Sung-Yi Yang', 'Chen-Hsun Weng', 'Gwo-Bin Lee']
I am writing a research paper on a idea A suction-type, pneumatic microfluidic device for rapid DNA extraction
[{"from": "human", "value": "I am writing a research paper on a idea A suction-type, pneumatic microfluidic device for rapid DNA extraction"}, {"from": "gpt", "value": "Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely: This current study presents a new miniature, integrated system capable of rapid extraction of genomic DNA (gDNA) from saliva samples. Three major components of the DNA extraction chip, including six symmetrical, normally-closed microvalves, a sample transport/mixing unit and a waste unit are integrated in this device. The microfluidic DNA extraction chip was made of CNC machining and PDMS casting processes to integrate a liquid channel layer, an air chamber layer and a glass layer on a single chip. Liquid samples can be transported and mixed by the suction-type sample transport/mixing unit by using micropumps, micromixers and normally-closed microvalves. Experimental results shod the pumping rate can be as high as 400 \u00b5L/min at an applied pressure of \u221270 KPa for an air chamber with a depth of 1.5 mm. A mixing efficiency as high as 96.5% can be achieved within 3 sec. Experimental data also shod that gDNA with an average concentration of 45 \u00b1 3 ng/\u00b5L and an optical intensity (OD) value (260/280) of 1.5 \u00b1 0.2 from ten measurements can be successfully achieved within 25 minutes. Consequently, the proposed miniature system can provide a porful platform for automatic, rapid DNA extraction."}]