Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely: This communication presents a pipeline for Multi-Angle TIRF calibration from the measurement of the incident angle to the model validation. This problem is of major importance when dealing with 3D reconstruction methods from a set of MA-TIRF acquisitions since the reconstruction accuracy highly depends on the agreement beten the theoretical model and the physical system. One main issue is then to build phantom samples with known geometry, or known properties, in order to adjust and/or validate the model.  describes such a calibration procedure using a lens as phantom sample and proposes a new model validation experiment based on a dual-color co-localization.
['Emmanuel Soubies', 'Sébastien Schaub', 'Agata Radwanska', 'Ellen Van Obberghen-Schilling', 'Laure Blanc-Féraud', 'Gilles Aubert']
I am writing a research paper on a idea A framework for multi-angle TIRF microscope calibration
[{"from": "human", "value": "I am writing a research paper on a idea A framework for multi-angle TIRF microscope calibration"}, {"from": "gpt", "value": "Thats a unique idea.You should consider the following idea to enhance your idea so that it would impact uniquely: This communication presents a pipeline for Multi-Angle TIRF calibration from the measurement of the incident angle to the model validation. This problem is of major importance when dealing with 3D reconstruction methods from a set of MA-TIRF acquisitions since the reconstruction accuracy highly depends on the agreement beten the theoretical model and the physical system. One main issue is then to build phantom samples with known geometry, or known properties, in order to adjust and/or validate the model.  describes such a calibration procedure using a lens as phantom sample and proposes a new model validation experiment based on a dual-color co-localization."}]