Ved at binde til dette site hæmmer pleuromutilin peptidyl transfer, blokerer P- site- interaktioner og forhindrer den normale funktion af ribosomets aktive 50S- del.
By virtue of binding to this site, pleuromutilins inhibit peptidyl transfer, partially block P-site interactions, and prevent normal formation of active 50S ribosomal subunits.