Sensitivity of the α-alanine antibody. Immunoblots of serial dilutions of lysates from HEK 293 cells transfected with plasmid encoding A17-PABPN1 probed with α-Alanine antibody. The total amount of lysate ( μg protein) loaded in each lane is indicated below the immunoblot panels. Blots were probed with α-PABPN1 to detect endogenous and recombinant PABPN1 protein and α-Tubulin was used to detect tubulin as a loading control.