Adhesion and internalization percentages with (a) LLC-MK2 cells and (b) murine macrophages after filipin treatment (1, 3, and 6 nM) for 30 min before the addition of the parasites (50 : 1) for 10 minutes. At 6 nM filipin, the adhesion of the parasites to the LLC-MK2 cells was slightly decreased, and internalization was significantly inhibited. In macrophages, internalization was inhibited by 85%. The data shown are the means ± SE of duplicated points from three independent experiments. * P