Figure 1: Treatment of HT-29 cells with UV stimulates phosphorylation of p53 at Ser15, detected by PathScan® Phospho-p53 (Ser15) Sandwich ELISA kit #7365, but does not affect the level of total p53 protein detected by PathScan® Total p53 Sandwich ELISA kit #7370. OD450 readings are shown in the top figure, while the corresponding Western blot using Phospho-p53 (Ser15) Mouse mAb #9286 (right panel) or p53 Mouse mAb #2524 (left panel), is shown in the bottom figure.