Identification of H1 human embryonic stem cell (ESC) line and early embryonic body inducement. ( a – d ) Immunofluorescence of ESC markers of H1 embryonic stem cell line treated with ( a ) NANOG; ( b ) OCT4; ( c ) SOX2; ( d ) SSEA4. ( e – h ) Fluorescence-activated cell sorting analysis of the H1 embryonic stem cell line. ( e ) Negative control. ( f ) OCT4; ( g ) SOX2; ( h ) SSEA4. ( i – l ) Representative image of early stage embryonic bodies. ( i ) Day 4; ( j ) Day 8; ( k ) Day 12; ( l ) Day 16. Arrows in ( k , l ) are pointing at the neural retina at the edges of the EBs. These data revealed that the H1 embryonic stem cell line expressed the embryonic marker. Neuroectodermal epithelia were about to emerge on day 8 and were evident from day 12.