SF-induced selective increase in the number of pro-inflammatory M1 macrophages in visceral fat of wild-type, but not Nox2-deficient mice A. A representative flow cytometry analysis showing an increase in the number of CD11c + M1 macrophages in visceral fat of a WT mouse exposed to SF for 2.5 weeks. Such SF-induced effect on the pro-inflammatory M1 subset was not observed in Nox2-deficient gp91 phox-/Y mice. B. Summary of 5 independent flow cytometry experiments. Notice that SF-induced decrease in the M2 subset was not significant at this stage of chronic SF. Data are mean ± SE; n=5 for each group.