Western blot analysis of Pan-Actin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600032, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: Rat heart tissue lysate<br /> Lane 1: Mouse smooth muscle tissue lysate<br /> Lane 1: Human skeletal muscle tissue lysate