Chromatin immunoprecipitations were performed with cross-linked chromatin from HaCaT cells treated with Human TGF-β3 #8425 (7 ng/ml) for 1 h and either Smad2 (D43B4) XP<sup>®</sup> Rabbit mAb #5339 or Normal Rabbit IgG #2729 using SimpleChIP<sup>®</sup> Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP<sup>®</sup> Human CDKN1A Intron 1 Primers #4669, SimpleChIP<sup>®</sup> Human ID1 Promoter Primers #5139, and SimpleChIP<sup>®</sup> Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.