Silence of FOXO1 mitigates the inhibitory effects of miR‐5188 knockdown on glioma cell proliferation, invasion and migration. MTT assays (A), EdU incorporation assays (B), flow cytometry assays (C), transwell assays (D) and Boyden assays (E) were performed in U251 or U87 cells treated with control sequence, FOXO1 siRNA or miR‐5188 inhibitor, as indicated. Scale bars, 100 μm. F, Western blotting analyses were applied to measure the protein levels of FOXO1, P‐PI3K, PI3K, P‐AKT, AKT, CCND1, CDK4, c‐JUN, N‐cadherin and vimentin in U251 or U87 cells treated with control sequence, FOXO1 siRNA or miR‐5188 inhibitor, as indicated. G, Western blotting analysis analyses were applied to measure the protein levels of FOXO1, P‐PI3K, PI3K, P‐AKT, AKT, CCND1, CDK4, c‐JUN, N‐cadherin and vimentin protein levels in U87 and U251 cells transfected with control sequence or FOXO1 siRNA. * P