Western blot analysis of extracts from PC-3 cells and wild-type (wt) and <i>Nf2</i> knockout (-/-) mouse embryonic fibroblasts (MEFs), untreated (-) or λ phosphatase-treated (+), using Phospho-Merlin (Ser518) (D5A4I) Rabbit mAb (upper), Merlin (D3S3W) Rabbit mAb #12888 (middle), or Ezrin/Radixin/Moesin Antibody #3142 (lower). (MEF wt and MEF <i>Nf2</i> (-/-) cells were kindly provided by Dr. Andrea McClatchey, MGH Cancer Center and Harvard Medical School, Charlestown MA).