Western blot analysis of extracts from HT-29 cells transfected with 100 nM SignalSilence<sup>®</sup> Control siRNA (Unconjugated) #6568 (-), SignalSilence<sup>®</sup> IRF-7 siRNA I #13139 (+) or SignalSilence<sup>® </sup>IRF-7 siRNA II #13291 (+). Twenty-four hours after transfection, cells were treated with Human Interferon-α1 (hIFN-α1) #8927 (10 ng/ml, overnight; +) and analyzed by western blot using IRF-7 (D2A1J) Rabbit mAb #13014 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The IRF-7 (D2A1J) Rabbit mAb confirms silencing of IRF-7 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.