Identification of cis-regulatory motifs in promoter sequences of AP-1 bTM genes.Yeast AP-1 bTMs were characterized using the procedure presented in Figure 1. They are represented here using the following color code: Yap1p bTM in S. cerevisiae (SCERE) in purple, Cgap1p bTM in C. glabrata (CGLAB) in orange, Cap1p bTM in C. albicans in green. Promoter sequences of genes were analyzing using a combination of five different algorithms (BEAM, PRISM, SPACER, Oligo-Analysis and MEME) and applying a filter procedure to select the most significant motifs (see Material and Methods and Text S3). 12 motifs were identified in SCERE, 7 motifs in CGLAB and 8 motifs in CALB. They are presented in Text S4. In each species, these motifs were combined and consensus sequences are shown here (SeqLogo representations). A unique consensus MTKASTMA was observed in promoters of SCERE and CALB genes and two consensuses (MTTASSTAA, ATTACHAAW) were observed in promoters of CGLAB genes (where M designates A or C, K designates G or T, S designates C or G and W designates A or T). Percentages of genes in each AP-1 bTMs that exhibit those consensuses are indicated below the SeqLogo representations, with the associated enrichment p-value (see Materials and Methods). Highly conserved positions between the consensuses are underlined. They are predicted to strongly interact with the TF DNA binding domain, based on structural inspection of the Pap1p/DNA complex (see Figure 4).