Efficacy of PRKD2 silencing. Knockdown of PRKD2 expression in U87MG cells was performed by RNA interference using two different siRNA constructs (Oligofectamine was used to transfect 20 nM siRNA). Silencing efficacy was analyzed by (A) qPCR two and four days post transfection and (B) Western blotting three and five days post transfection. Results in (A) represent mean±SD of three different experiments. Results in (B) show one representative Western blot out of five independent experiments. Numbers represent relative optical densities of PRKD2 protein normalized to loading controls.