Flow cytometric analysis of ZAP70 was done on Jurakt cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-27, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).