DR4 protein expression depends on SOCS3 Individual bands of the Western blotting analysis were quantified using Image Lab 3.0 software and compared with protein expression levels. (A) Effect of pCI-SOCS3 on ACHN cells. Overexpression of SOCS3 suppressed the DR4 expression by IFN in ACHN cells. (B) Effect of SOCS3-siRNA on 786-O cells. DR4 protein expression was dependent on SOCS3 expression. The results are expressed as the relative mean ratio ± SE of at least three independent determinations. * p