Temporal profiles ofBacillus pumilus-like RR-3 strain in LB + 0.5 mM DBT media. (a) Growth curve assessed by optical density (OD at 600 nm) of cell mass in culture at different times. (b) Residual DBT concentration (μM) in culture media at the same time points. As indicated by the legend in the graph, the four different growing conditions were established in 10-mL cultures by varying the presence of MgSO4 (6.65 μM) in the medium, combining with two amounts of inoculum cells. The RR-3 inocula were established by adding pre-cultured cell suspensions, with 0.500 and 1.500 OD readings at 600 nm, at 5% of final culture volume. Treatments were applied in triplicate and the experiment was repeated at least once, with same results. Hexane extraction and HPLC analysis were used for detection of both compounds. Practically the whole amount of the applied DBT could be detected at time zero.