PCR product melting curves were obtained for real-time PCR reactions performed using SimpleChIP<sup>®</sup> Human Bcl-2 Promoter Primers. Data is shown for both duplicate PCR reactions using 20 ng of total DNA. The melt curve consists of 80 melt cycles, starting at 55°C with increments of 0.5°C per cycle. Each peak is formed from the degradation of a single PCR product.