Effects of UPC on AGS and TRPM7 overexpressed HEK293 cells. (A) Representative I-V relationships of the effect of UPC on TRPM7 currents in AGS cells. (B) Representative I-V relationships of the effect of UPC on TRPM7 currents in HEK293 cells. (C) TRPM7 channels were treated or not treated with tetracycline for 1 day. Cells were incubated with UPC, followed by MTT assay. A voltage ramp from +100 to -100 mV was applied from a holding potential of -60 mV. The figures show mean ± SEM. *P < 0.05, **P < 0.01.value relative to that of the untreated cells which is set to 100%. The figures show mean ± SEM. *P < 0.05, **P < 0.01.