Effect of inhibition of Pim-2 in K562, RPMI-8226, H1299 and A549 cells on the cell cycle regulatory protein expression levels. Western blot analysis of cell cycle regulatory proteins P53, P21, CDK2, pRb and Rb in control and pim-2 silenced cells. p21 was highly expressed in all the cell lines. GAPDH was used as endogenous control. CDK2, cell dependent kinase 2; Rb, retinoblastoma; p, phosphorylated; siRNA, short interfering RNA.