PRC1 localization in Fcp1-depleted cells. ( A ) Asynchronously growing control (Cont. siRNAs) or Fcp1-siRNAs-treated (Fcp1 siRNAs) HeLa cells were fixed and stained for PRC1 (red), α-tubulin (green) and DNA (blue). ( B ) Control (Cont. siRNAs) or Fcp1-siRNAs-treated (Fcp1 siRNAs) HeLa cells, previously arrested at prometaphase, were released into fresh medium in the presence of the proteasome inhibitor MG132. After 30 min incubation, the Cdk1 inhibitor RO3306 was added, cells were fixed and stained for PRC1 (red), α-tubulin (green) and DNA (blue) at indicated time points of further incubation. Scale bars, 10 μm. Data shown are representative of three independent experiments. DOI: http://dx.doi.org/10.7554/eLife.10399.006