EGb inhibited IL-1-induced JNK but not p38 or ERK activity. Chondrocytes were pretreated with various concentrations of EGb for 72-1 for 15 min. The total cell lysates were analyzed by western blot using antibodies against un-phosphorylated or phosphorylated JNK (A), p38 (B) or ERK (C). The fold inductions of densitometric intensity of individual proteins equalized with β-actin, compared to those in un-stimulated samples, from more than 3 independent experiments using different donor cells (n = 5 for JNK and n = 3 for ERK and p38) are shown (Fig. 4D).