Western Blot of Rabbit anti-GFP antibody.   Lane 1:  293FT cells transfected with CDK4 dominant negative (C-). Lane 2:  293FT cells poitive control (C+). Load:  25 µg per lane. Primary antibody:  GFP antibody at 1:400 for overnight at 4°C. Secondary antibody:  IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block:  5% BLOTTO overnight at 4°C. Predicted/Observed size:  27 kDa for GFP.