Thumbnail of Seminested PCR detection of Bartonella spp. DNA in Ixodes ricinus ticks fed on B. henselae–infected ovine blood at preceding stage. Lane M, 100-bp DNA molecular mass marker; lane Ags, salivary glands of a female adult fed on infected blood as a nymph; lane A, carcass of a female adult fed on infected blood as a nymph; lane Ngs, salivary glands of a nymph fed on infected blood as a larva; lane N, carcass of a nymph fed on infected blood as a larva; lane E, eggs laid by female adult fed on infected blood; lane L, larvae hatched from female adult fed on infected blood; lane T+, B. bacilliformis DNA; lane T–, nymph fed on uninfected ovine blood.