Western blot analysis using Goat anti Human IgG + IgA + IgM (H + L) (HRP) (43C-CB0991) | Western Blot of Human IgG, IgA, IgM Peroxidase Conjugated antibody. Lane 1: Human IgG. Lane 2: Human IgA. Lane 3: Human IgM. Load: 50 ng per lane. None. Peroxidase Goat secondary antibody at 1:1,000 for 60 min at RT. Block: Blocking buffer for 30 min RT. Size: Appropriate banding patern for Human IgG, IgA, and IgM is shown. Other band(s): Splice variants and isoforms.