Effect of the protein inhibitor on signal protein activities in GMECs. ( A ) GMECs were treated with 500 nM MK2206 (Akt inhibitor) for 60 min and then stimulated with 50 ng/mL EGF for 60 min, to investigate the phosphorylation level of Akt. ( B ) GMECs were treated with 10 µM <t>U0126</t> (MEK inhibitor) for 60 min and then stimulated with 50 ng/mL EGF for 60 min, to investigate the phosphorylation level of ERK1/2. ( C ) GMECs were treated with 20 µM U73122 (PLC-γ1 inhibitor) for 15 min and then stimulated with 50 ng/mL EGF for 60 min, to investigate the phosphorylation level of PLC-γ1. ( D ) The band intensity was shown by Image J software. Values are presented as means ± SD of three independent experiments. * Means p