Lesion profiling (A, B, C) and paraffin-embedded tissue blot characterization of prion protein (PrPSc) deposition at thalamic level (D, E, F). Tests were performed by using formalin-fixed brain from Tg338 mice (expressing the VRQ PrP ovine variant) inoculated with (A, D) ARR/ARR atypical case (B, E) bovine spongiform encephalopathy (BSE) brain from an ARR/ARR sheep (intracerebral inoculation), and (C, F) case S83. Each lesion profile was carried out by using 6 animals. Detection of PrPSc was achieved by using the monoclonal antibody Sha31.