FISH mapping of different repeat sequences in nuclei isolated using the chromosome isolation procedure. (A) K . brevis and (B, C) K . mikimotoi . DAPI-stained DNA (blue) and in situ hybridization of the 45S rDNA digoxigenin-labeled probe (green) in combination with (A) the Dy547-labeled telomeric probe (red) or (C) the biotin-labeled (AG) 10 probe (red). Note the largely disturbed nuclear morphology after FISH. Scale bar = 10 μm.