Trypsinogen Antibody - Dot Blot results of rabbit Anti-Trypsin Peroxidase Conjugated. Antigen: Trypsin. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. Blocking: MB-070 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Trypsin HRP 10µg/mL for 1hr at RT. Secondary Antibody: none. Imaging System ChemiDoc, Filter used: Chemi.