Analysis for effects of H 2 on the expression of p53 in H9c2 cells. (A–D) Overexpression of p53 in H9c2 cells. In the groups of P53 and pLVX, H9c2 cells were infected with p53-lentivirus or pLVX and were selected for 72 h. Then the cells were treated with 10 μM VK 3 and 75% H 2 for 8 h. In the groups of DMSO, VK 3 and VK 3 +H 2 , the cells were treated as the primary cardiomyocytes. (E–H) Inhibition of p53 in H9c2 cells. H9c2 cells were treated with 10 μM PFTα or equivoluminal DMSO for 16 h (DMEM group and PFTα), and then treated with 10 μM VK3 for 8 h (VK3 group and PFTα+VK 3 group). Western blot analysis was performed on 100 g cell lysates with antibodies against p53, Bax, or cleaved caspase-3. GAPDH was used as a loading control. Expressions of p53, Bax, and cleaved caspase-3 were, respectively, quantified as folds of GAPDH ( n = 3–4). The columns indicate the mean, and the bars indicate the SE. ∗ p