The combination of ruxolitinib, Navitoclax, and BV triggers a mitochondrial apoptosis pathway in HDLM-2 cells. The mitochondrial membrane potential was measured by the membrane-permeant JC-1 dye staining. After treatment (ruxolitinib, 250 nM; Navitoclax, 50 nM; and BV, 100 ng/mL) for 48 h, the HDLM-2 cells were incubated with JC-1 at a concentration of 10 µg/mL in medium at 37 °C, 5% CO 2 for 10 min and then washed with PBS. The samples were collected on a FACScan flow cytometer. Data are representative of three independent experiments.