HPRT expression in RIN and Jurkat T cells assessed by RT-PCR. (a) RIN cells unstimulated and treated for 24 h with IL-1β (25 U/ml) in the absence or presence of AzadC (2 μg/ml). (b) Similar protocol used with Jurkat T cells stimulated with 100 μM SIN. (c) RT-PCR of FMR1 and HPRT from fresh peripheral lymphocytes incubated for 24 h with SIN (100 μM) in the absence or presence of AzadC (2 μg/ml). RT-PCR of GAPDH was used as control.