The suppression of OCs on T-cell proliferation is IFN-γ and CD40L dependent Proliferation of CD4 + T cells stimulated by (A) α-CD3/CD28 Dynabeads at a T/Bead ratio of 2:1 or (B) allogeneic DCs at a T/DC ratio of 10:1 in Transwells in the absence or presence of autologous OCs with IFN-γ neutralizing or IFNGR1 blocking antibodies (20 μg/mL) for 4–6 d. (C) Proliferation of CD4 + T cells stimulated by allogeneic DCs under the contact coculture of autologous OCs with IFN-γ neutralizing, CD40 or CD40L blocking antibodies (20 μg/mL). (D) Expression of IFNGR1 and CD40 on OCs, as measured by flow cytometry. OCs coculutred with α-CD3/CD28 Dynabeads-activated CD4 + T cells in Transwell system were harvested by Trypsin/EDTA detachment, stained by antibodies and analyzed by flow cytometry. Representative results from 3 independent experiments are shown.