( A ) Representative immunofluorescence images of NFATc1 (red) in WT and Tmem178 −/− BMMs cultured with 10 ng/mL M-CSF and 50 ng/mL RANKL for the indicated days. DAPI (blue) was used as nuclear stain. ( B ) Western blot analysis of NFATc1 nuclear translocation in response to 50 ng/mL RANKL in WT and Tmem178 −/− preOCs for the indicated time points. Lamin B is shown as loading control, representative of three experiments. ( C ) Western blot analysis of NFATc1 nuclear translocation in response to 50 ng/mL RANKL in WT cells expressing empty vector pMX or Tmem178-HA for the indicated time points. Histone 3 is shown as loading control, representative of three experiments.