DJ-1 activates Wnt signaling by enhancing PLAGL2 expression. a Western blot analysis of PLAGL2 expression in HCT116, SW480, and SW620 cells stably transfected with DJ-1 cDNA, DJ-1 shRNA, or vector. b – f HCT116-DJ-1, SW480-DJ-1, and SW620 cells were transfected with two specific PLAGL2 (PL) siRNAs, respectively. Then, protein levels of activated-β-catenin, BMP4, CCND1, and PLAGL2 were determined by Western blot ( b ); Wnt activity was analyzed by TOP-Flash/FOP-Flash assay ( c ); proliferation of CRC cells was examined by CCK8 kit ( d ); proliferative activity was detected by colony formation assay ( e ); migration and invasion activity were assessed by transwell migration and matrigel invasion assays ( f , scale bar, 100 μm). Migration was analyzed at 24 h, invasion at 48 h. * P