Phylogenetic analysis of Toggenburg orbivirus (TOV) (shaded region) genome segment 2 by ClustalW alignment (16) and subsequent neighbor-joining tree construction by MEGA version 4 software (15). GenBank accession numbers are indicated for all orbivirus sequences used to construct dendrogram. BTV, bluetongue virus; EHDV, epizootic hemorrhagic disease virus; AHSV, African horse sickness virus. Scale bar indicates number of nucleotide substitutions per site.