Indirect immunofluorescence (IF) staining of live keratinocyte monolayers with pemphigus vulgaris auto-antibodies (A) Cryosections of human skin stained with biotinylated DSG3-specific monoclonal antibodies (Ab00153, Ab00155, Ab00158 and Ab00156). (B) Staining of human epidermis with biotinylated PVB28 (Ab00158) in the presence (with) or absence (w/o) of Ca2+. (C) Binding of a panel of pemphigus auto-antibodies to DSG3-coated ELISA plates in the presence or absence of EDTA.