Effect of macrophage-derived MVs on insulin-stimulated activation of NF-κB in human adipocytes. a , b . Relative protein levels of nuclear NF-κB p65 in human mature adipocytes ( a ) and differentiated cells ( b ) were analyzed by western blotting. Human adipocytes were treated with indicated MVs for 24 h, and then incubated with 0, 1, 10, or 100 nM of insulin for 20 min. NF-κB p65 and histone H3 levels in nuclear lysates were determined by immunoblotting. Quantification of NF-κB p65 protein level is shown below. Western blot images were analyzed using Bandscan software, and statistical analysis is presented below. * P