Effects of the single amino acid mutant on class B scavenger receptor I mRNA and protein expression. A: Effect of the single amino acid mutant on SR-BI protein expression. Huh7-siSR-BI cells were seeded in 24-well plates, cultured overnight, and transfected with pcDNA3.1 (NC), pcDNA-SR-BI, or pcDNA-SR-BI/S112F. Cells were harvested 72 h after transfection and lysed with RIPA cell lysis buffer; Western blot was performed to analyze the expression of the SR-BI protein. B: Effect of the single amino acid mutant on the SR-BI mRNA level. Huh7-siSR-BI cells were seeded in 24-well plates, cultured overnight, and then transfected with pcDNA3.1 (NC), pcDNA-SR-BI (WT), or pcDNA-SR-BI/S112F (S112F). Cells were harvested 72 h after transfection and RNA was isolated and the level of the SR-BI mRNA was analyzed using qRT-PCR ( a P