Validation of SIRT1 down-regulation in TDP-43, FMRP of STAU1 silenced SH-SY5Y cells. A–C , SH-SY5Y cells were transfected with scrambled control siRNA, TDP-43 siRNA, FMRP siRNA, or STAU siRNA. Total RNAs were prepared and analyzed for SIRT1 expression by RT-PCR ( left panels ) and Western blotting ( middle panels ). The intensities of the gel bands were quantitated using the Quantity One (BioRad) program ( right panels ). The mRNA levels were normalized to those of GADPH and α-tubulin served as a loading control for the Western blotting. Error bars represent S.D. of triplicates. *, p