Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence<sup>®</sup> Control siRNA (Unconjugated) #6568 (-), SignalSilence<sup>®</sup> Atg14 siRNA I #6286 (+) or SignalSilence<sup>®</sup> Atg14 siRNA II #6287 (+), using Atg14 Antibody #5504 (upper) or β-Tubulin (9F3) Rabbit mAb #2128 (lower). The Atg14 Antibody confirms silencing of Atg14 expression, while the β-Tubulin (9F3) Rabbit mAb is used as a loading control.