Suppression of RANKL- induced expression of NFATc1 and TRACP by salubrinal in RAW264. 7 cells. A : Salubrinal-driven suppression of NFATc1 expression in RANKL-stimulated bone marrow-derived cells. B : Dose dependent suppression of NFATc1 in response to 1 – 20 μM salubrinal. β-actin was used as a loading control. C : Relative mRNA levels of NFATc1 and TRACP in response to 1 – 20 μM salubrinal. The mRNA levels are normalized by the mRNA level of the sample without salubrinal in the absence of RANKL stimulation.