Phospho-KDR antibody used in western blot to detect phosphorylated KDR/FLK1 in HMVEC lysate. Endothelial cells were stimulated with 50ug/ml VEGF for 5min and Ab used at 1:500. Predicted molecular weight: ~152 (immature), 180-200 kDa (intermediate) and 220-230 kDa (mature).