Fig. 2. Fluorescent multiplexing enables the quantification of phosphoproteins on a single blot. The Phospho-Specific Mouse Anti-EGF Receptor pSer1047 (#VMA00746) and anti-total Mouse AntiEGF Receptor (#VMA00061) were incubated together. hFAB Rhodamine Anti-Tubulin was added with the fluorescent secondary antibodies. The fluorescent images were captured using the ChemiDoc MP Imaging System.