Vsig4 gene transcription is repressed Dnmt3a-mediated DNA methylation. The C57BL/6 WT mice were infected with MHV-3 (100 PFU/mouse), a the expression of VSIG4 on PEMs at 0 h and 12 h PI was detected by flow cytometry. b VSIG4 protein level in liver tissues was analyzed by western blot. The BMDMs were treated with proinflammatory factors for 12 h. c Vsig4 gene transcription was detected by qRT-PCR. d VSIG4 protein expression was evaluated by western blot. e The BMDMs were treated with Dnmts inhibitor-AZAdC (10 μM) for 72 h in advance, cells were then further added with proinflammatory mediators for 12 h, the expression of Dnmt3a and VSIG4 was assessed by western blotting. f Luciferase activity of the lysates from RAW264.7 cells transfected with unmethylated or M.SssI methylated pGL3-basic vector and the -840/+1 Vsig4 promoter constructs. Error bar, s.e.m. * p