Rat IgG Antibody - Western Blot of Anti-Rat IgG (H&L) (GOAT) antibody Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule Lane 2: Rat IgG F(c) Fragment Lane 3: Rat IgG Fab Fragment Lane 4: Rat IgM Whole Molecule Lane 5: Rat Serum All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (GOAT) antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.