DEX effects on RA synovial fibroblast annexin I mRNA. Panel (a): RNA extracted from human RA FLS was subjected to competitive <t>RT-PCR</t> with (lanes 2–6 and 8–12) or without (lanes 1 and 7) <t>mimic.</t> Lanes 1–6: control; lanes 7–12: DEX 10 −7 M for 24 hours. Annexin I mRNA content was greater in DEX-treated than vehicle-treated cells. Representative of n = 4 separate experiments from 4 separate RA donors. Panel (b): the log of the ratios of the annexin I and mimic PCR product band intensities were graphed as a function of the log of the amount of mimic added to the reaction. Increased annexin I mRNA is seen in DEX-treated RA synovial fibroblasts. Representative of n = 4 separate experiments from 4 separate RA donors.