MS induces a muscle type switch to slow-twitch fibers After crush injury and MS treatment, the quadriceps was retrieved and analyzed by western blot or histology. We found that the expression of muscle proteins was increased after MS treatment. (A) Desmin nearly doubled its expression levels, and (B) a specific increase in MyH type 1 could be detected. (C) No significant difference in MyH type 2 was found by WB. (D, E, F) Staining was performed with anti-myosin-heavy-chain-slowtwitch/FITC-anti-mouse-IgM (green), anti-myosin-heavy-chain-fast-twitch/Cy3-antimouse-IgG (red), and DAPI (blue). When compared with intact control quadriceps, no fiber type change was found in injured muscle without MS stimulation. (E) On the other hand, a shift to type 1 fibers was verified in MS-treated samples. (F) MyH type 1 expression was up to 3-fold higher in MS-treated samples than in native muscle. (G) As expected, a relative decrease in MyH type 2 was detected. +MS indicates magnetic stimulation treatment, whereas -MS represents the unstimulated controls (* P