IKKα-targeting miRNAs affects noncanonical and canonical NF-κ.B gene expression (a) Real time PCR analysis of ELC mRNA in monocytes and macrophages, normalized to GAPDH, data representative of at least three independent experiments. Error bars, +/−standard deviation of the mean. (b) Anti-RelB immunoblots of lysates from macrophages (MΦ) treated with LPS for indicated time course (in hours). (c). Real time PCR analysis of ELC mRNA in monocytes, macrophages, or LPS-treated macrophages, normalized to GAPDH. Data representative of at least three independent experiments. Error bars, +/−standard deviation of the mean. (d)(e)(f), Macrophages (MΦ) were transfected with miRNA mimics or control mimic and 24 hours after transfection, cells were or were not challenged with LPS (1μg/ml) for another 24 hours. (d) Immunoblot showing IKKα protein level in corresponding protein samples for (e, f). (e) ELC and (f) A20 mRNA expression as measured by real time PCR, normalized to GAPDH mRNA. The relative fold increase in LPS treated cells compared to untreated cells is shown for (e) and (f) . Error bars, +/− range based on standard deviation of the mean. Data representative of at least three independent experiments. TRADD and β-actin blots indicate loading of lanes, (b, d).