"IL-17A expression in CD4+ Foxp3+ Tregs requires TLR-2 signaling in Tregs during C. albicans infection in vivo. WT C57BL/6 or Tlr-2−/− mice infected with C. albicans, and tissues were isolated as in ""1a"". Flow cytometric plots gated on CD4+ cells showing IL-17A expression (a), and statistical analyses of IL-17A expression in CD4+Foxp3+ cells (b) are shown. Data represent triplicate experiments. (c,d) Foxp3YFPcre or Myd88 fl°x Foxp3YFPcre mice were infected, and cells were isolated as in ""1a"". Flow cytometric plots gated on or CD4+ cells showing IL-17A expression (c), and statistical analyses of IL-17A expression in CD4+Foxp3-cells (d, upper panel) and CD4+Foxp3+ cells (d, lower panel) are shown."