Immunoprecipitation of phospho-NF-κB p65 (Ser536) from HeLa cells treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (20 ng/ml, 5 min) using Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2) or Phospho-NF-κB p65 (Ser536) (E1Z1T) Mouse mAb (lane 3). Lane 1 represents 10% input. Western blot was performed using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033.