Cointegrate formation between pRMH762 and R388. The R388 backbone is drawn to scale from GenBank accession no. BR000038 with key resistance genes, genes involved in replication (repA), and genes involved in conjugative transfer (tra) shown as arrows inside the circular backbone. Arrows pointing toward the circular backbone indicate the location of 20 mapped R388::pRMH762 cointegrates. The precise location and orientation of nine cointegrates were determined by sequencing. An arrow labeled 1 indicates a cointegrate with tnp26 facing clockwise, while an arrow labeled 2 indicates a cointegrate with tnp26 facing counterclockwise. An asterisk denotes the cointegrate used in the construction of R388::IS26.