Inhibitory effects of the crude polysaccharides from the Celluclast enzyme digest (CCP) on NF-κB and MAPK activation in LPS-stimulated RAW 264.7 cells. RAW 264.7 cells were treated with LPS (1 µg/mL) or CCP (100 µg/mL) for 10 or 20 min. (A) and (B) Cytosolic cell lysates, (C) and (D) nuclear protein extracts (40 µg) were resolved by 10% SDS-PAGE, transferred to nitrocellulose membranes, and probed with antibodies against NF-κB p50 and p65. The proteins were then visualized by ECL. (E) and (F) Effects of CCP on MAPK activation induced by LPS in RAW 264.7 cells. RAW 264.7 cells were treated with LPS (1 µg/mL) or CCP (100 µg/mL) for 10 or 20 min. Total protein (40 µg) was separated by 10% SDS-PAGE, transferred to nitrocellulose membranes, and probed with antibodies against ERK and p38. The proteins were then visualized by ECL. The intensity of the bands was measured by ImageJ software. Relative amounts of density ratio represented the relative intensity of each band against that of the standard protein. The results shown are representative of those obtained from three independent experiments. Each data point represents the mean ± SEM ( * P