Pharmacologic blockade of PR with RU486 negatively regulates VEGF-A expression in DSCs for decidual angiogenesis and EEVSF The pregnant mice were treated with control buffer (Control, C) and RU486 (8 mg/kg) at 5.5 dpc, and the uteri were harvested at 6.5 dpc and analysed. A. Comparison of VEGF-A expression in the uteri of VEGF +/LacZ mice. Each numbered region (square-dotted line) is magnified in right side. Scale bars, 500 µm. B. Semi-quantitative RT-PCR showing mRNA expressions of VEGF-A and VEGFR2 (VR2). Each line indicates gene transcript of VEGF-A 120 , VEGF-A 164 and VEGF-A 188 . C. FACS analysis showing CD31 + /CD45 − EC populations from the endometrium. Each group, n = 4. D. Images comparing CD31 + BVs in the ULR, CTR and AMR. Scale bars, 100 µm. E, F Comparisons of CD31 + BVD (%) in the ULR, CTR and AMR, and numbers of different sized VSFs in the CTR. Each group, n = 4. * p