Knockdown of p27 suppresses autophagy and increases apoptosis in glucose-deprived ( GD ) cardiomyocytes. A , Western blot shows lysates from cardiomyocytes treated with and without shRNA to knock down p27 versus control shRNA ( NT ) in the presence or absence of glucose. B , shRNAp27 reduced p27 and LC3-II levels and increased p62 levels. Apoptosis marker cleaved caspase 3 was increased in cardiomyocytes undergoing p27 knockdown. C , representative photomicrographs show cardiomyocytes fixed and stained for LC3 ( green ), α-actinin ( red ), and nuclei ( blue ). D , histogram shows the number of LC3 + dots/cell. E , TUNEL-positive nuclei are quantified. *, p