PC derived from DP IgG1 MBC are enriched in high affinity mutations. VDJ nucleotide (nt) and amino acid (aa) sequences were compared between IgG1 MBC subsets and their IgE and IgG1 progeny cells. IgG1 MBC subsets were purified from 10 weeks OVA-PEP1 immunised TBmc mice, and transferred together with CD4 memory T cells into Rag1 KO recipient mice. The recipient mice were immunised with OVA-PEP1. Spleen and bone marrow (BM) of the recipient mice were collected 2 weeks later. The VDJ repertoires of parental IgG1 MBC subsets and their IgG1 and IgE progeny were analysed using next generation sequencing. a , b Average number of nt a and aa b mutations per sequence. c Percentage of sequences containing R97T, N100aS/T or A101T high affinity CDR3 mutations. a – c Data are average ± SEM of three mice per group. d Enrichment in the percentage of CDR3 sequences containing 2 or 3 high affinity aa per sequence (hi aff aa/seq) in the IgE and IgG1 progeny of DP IgG1 MBC (M1, M2 and M3 indicate three recipient mice). Data are representative of two independent experiments