Effects of NFATc1 induction or depletion on different genes involved in the RANK–RANKL pathway. Cells were untransfected and RANKL-induced. QPCR results of ( A ) NFATc1, Myc and Acp5 ; ( B ) Jun, Esr1 and Egr 1; and ( C ) CtsK, Hnf1a, Smad5 and Stat4 . Cells were NFATc1-knockdown and RANKL-induced. QPCR results of ( D ) GATA2 and Runx2 ; ( E ) NFATc1, CtsK, Acp5, Stat4, Hnf1a and Egr1 ; ( F ) Smad5, Esr1 and Jun ; and ( G ) RhoA, DC-STAMP, MMP9, MITF, FOS and OSCAR . The mRNA expression levels were presented as relative values to those expressed in siRNA-NC transfected or untransfected cells arbitrarily set at 1.0. The results shown are the means ± SD of three experiments (each of which was performed in triplicate). * p