"Induction of the TRIM5α-activated antiviral state does not require a productive infection. (a) Representative FACS dot plots of TRIM5-KO or control Jurkat cells infected or not with AT-2- or MetOH (vehicle)-treated NRC10 DsRed , followed by challenge with NRC1 GFP 48 h later. The % of GFP + cells are shown in bold. Bar graphs showing the percentage of infected cells following infection of T5KO or control Jurkat cells with NRC1 GFP or NRC10 GFP, 48 h after a first infection with NRC10 DsRed or EC8-2 DsRed treated with AT-2 or with the vehicle only (M). Means with SD are plotted. Numbers on the bars represent the -fold inhibition relative to the relevant mock control. (b) Representative dot plots of TRIM5-KO and control THP-1 cells infected or not with AT-2- or vehicle-treated NRC10 DsRed , or with ""empty"" psPAX2-vector containing NRC10 CA (NRC10_EV), followed by NRC1 GFP challenge 48 h later. The % of GFP + cells are shown in bold. Bar graphs showing the percentage of infected cells following infection of T5KO or control THP-1 cells with NRC1 GFP, 48 h after a first infection using NRC10 DsRed or EC5 DsRed treated with AT-2, with the vehicle only, or using NRC10 or EC5-2 ""empty vectors"". Means with SD are plotted. Numbers on the bars represent the -fold inhibition relative to the relevant mock control. Shown in all bar graphs of this figure are representative experiments performed in triplicates and repeated at least three times. Individual values are included (black symbols)."