Flow cytometric analysis of P2X7 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-99, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; blcak).