Identification of EPCs developed from cord blood CD34 + cells. The purification of CD34 + cells after immunomagnetic sorting (A). Representative results of flow cytometric analysis of cell markers on EPCs (B, C). Adherent cell Dil-AcLDL uptake and FITC-UEA-1 binding were determined by fluorescent microscopy. Double-positive cells, which appeared yellow in the overlay, were identified as differentiating EPCs (D). White bars: 10 μm.