Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either Nanog Antibody or Normal Rabbit IgG #2729 using SimpleChIP<sup>®</sup> Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP<sup>®</sup> Human Oct-4 Promoter Primers #4641, SimpleChIP<sup>®</sup> Human Sox2 Promoter Primers #4649, and SimpleChIP<sup>®</sup> Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.