Western blot analysis of extracts from HeLa cells transfected with non-targeted (-) or Akt2 (+) siRNA. Akt2 was detected using Akt2 Antibody #2962, and p42 was detected using p42 MAPK Antibody #9108. The Akt2 Antibody confirms silencing of Akt2 expression, and the p42 MAPK Antibody is used to control for loading and specificity of the Akt2 siRNA.