Streptavidin-based purification of Drs2p and Cdc50p.N-terminally Bad-tagged Drs2p and His10-tagged Cdc50p were purified onto a streptavidin column, and eluted by TEV cleavage. (A) Coomassie Blue stained 10% SDS-PAGE. (B, C, D) Immunodetection using a Biotin probe, a α-Drs2p antibody and a Histidine probe, as indicated. T, total membranes before solubilization; S, DDM-solubilized fraction; FT, flow-through; R, Streptavidin Sepharose resin before addition of TEV; RTEV, Streptavidin Sepharose resin after incubation with TEV for 16 h at 6°C; E, fraction eluted from the resin after incubation with TEV. Drs2p(t), truncated form of Drs2p; Cdc50p(g), glycosylated form of Cdc50p; His6-TEV, N-terminally hexahistidine-tagged TEV.