BAT3 associates with Derlin2. A. HA-Ri332 was synthesized in a rabbit reticulocyte lysate in the presence or absence of canine pancreatic microsomal membranes (MM). Following NP40-mediated lysis, Ri332 was retrieved by immunoprecipitation. The immunoprecipitate and input samples were blotted for either BAT3 or HA as indicated. B. 293T cells were subjected to NP40 lysis, followed by retrieval of the indicated proteins. Pre-immune serum served as a control. The eluates were blotted for BAT3, as were the input control samples. C. Immunofluorescence of Hela cells using antibodies against endogenous BAT3 (green) and Derlin2 (red). Scale bar = 5 µm. D. 293T cells were transiently transfected with the indicated constructs, subjected to NP40 lysis followed by an immunoprecipitation for Derlin2. Immunoprecipitates were blotted for BAT3. Input samples were blotted for BAT3 and Derlin2. The asterisk indicates non-specific polypeptides.