Figure 2: The relationship between protein concentration of lysates from untreated and hFGF-treated Hep G2 cells and the absorbance at 450 nm as detected by the PathScan<sup>®</sup> Phospho-FGF Receptor 4 (panTyr) Sandwich ELISA Kit #69193 is shown. Starved Hep G2 cells (85% confluence) were treated with Human FGF acidic (hFGF acidic) #5234 (100 ng/ml, 2-5 min, 37°C) and then lysed.