Effect of 100 µM SNAP treatment during 96 h on XTC.UC1 and BCPAP cell lines ( N = 5 per cell line). 1A: Nitric oxide measurement with the FACScan cytometer (10 µM final DAF2/DA dye). Results are expressed in arbitrary fluorescent units as mean values±SEM. * P ≤0.05 versus T0. ** P ≤0.05 versus T0 and T72. 1B: Evaluation of protein nitration by Western blotting using antibody raised against nitrotyrosine-modified proteins. Immunoblots are quantified by densitometric analysis and expressed in arbitrary units (relative to α-tubulin) as mean values±SEM. * P ≤0.05 versus T0.