GelRed™ Left: Preload 10x. Left Middle: Preload 100x. Right Middle: Precast. Right: Post-stain. Photographed on a UV transilluminator 365nm with an EtBr filter. Lane 1: 1μl of Hyperladder 1kb™ (Bioline) 2: 1μl of Hyperladder IV ™ (Bioline) 3: 0.3μg Lambda DNA HindIII marker2 ™ (ThermoScientific™) 4: 580ng of PCR product 5: 27ng of PCR product 6: 8ng of PCR product (as measured on a Qubit ™ Fluorometer)