Foxc2 overexpression induces activation of ERK and PI3K in BMSCs. (A , B) BMSCs were transfected with Lv-GFP, Lv-Foxc2 or Lv-Foxc2 following pretreatment with 50 μM PD98059 or 10 μM LY294002 for 1 h. Cell lysates harvested after 2 weeks of culture were analyzed by SDS-PAGE and Western blot with anti ERK or anti-PI3K antibody. The result of ERK or PI3K expression was indicated by the ratio of band intensity of ERK or PI3K with β-actin. *p