Figure 1. Tau protein is expressed in mouse brain but absent in HeLa cells. The relationship between lysate protein concentration from mouse brain and HeLa cells and the absorbance at 450 nm using the FastScan™ Total Tau ELISA Kit #57519 is shown in the upper figure. The corresponding western blot using a Tau antibody is shown in the lower figure. Mouse brain tissue or unstarved HeLa (90% confluence) cells were collected and then lysed.