Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence<sup>®</sup> Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence<sup>®</sup> Akt siRNA II (+), using Akt (pan) (11E7) Rabbit mAb #4685 and β-Actin (13E5) Rabbit mAb #4970. Akt (pan) (11E7) Rabbit mAb confirms silencing of Akt expression, while β-Actin (13E5) Rabbit mAb is used to control for loading and specificity of Akt siRNA.