JH and insulin regulate starvation resistance.A. RNAi-aided knockdown in the expression of genes coding for JHAMT or Met extended the starvation survival. Percent beetles survived during starvation after injection of control malE, JHAMT, or Met dsRNA respectively into 40 newly emerged male adults are shown. Starvation survival was recorded from day 4 to day 14. B. RNAi-aided knockdown in the expression of genes coding for ILP2 extend starvation survival. Percent beetles survived during starvation after injection of control malE, ILP1, ILP2, ILP3, or ILP4 dsRNA respectively into 40 new emerged male adults are shown. Starvation survival was recorded from day 4 to day 14. C. Bovine insulin can rescue the starvation survival of JHAMT or ILP2 RNAi beetles. Percent beetles survived after injection of control malE, JHAMT, ILP2 dsRNA into day 0 male adults followed by injection of either 25 mM HEPES or 25 mM HEPES containing 10 mg/ml insulin into day 5 adults are shown. The starvation survival was recorded from day 9 to day 14. D. JH III rescues starvation survival in JHAMT but not in ILP2 RNAi beetles. Shown are percentages of beetles survived after injection of control malE, JHAMT, ILP2 dsRNA into day 0 male adults followed by topical application of either acetone or 10 mM JH III in acetone on days 3, 5, and 7. The starvation survival was recorded from day 9 to day 14.