Ad- p53 14/19, Ad- p53 22/23 and Ad-wt p53 enhance doxorubicin (Doxo)-mediated apoptosis in HT1080 fibrosarcoma cells. Cells were infected with NCV, Ad-wt p53 , Ad- p53 22/23 or Ad- p53 14/19 for 3 days alone or in combination with 0.1 μ g/ml doxorubicin. Cells were then stained with PI and apoptosis was assessed with sub-G1 profile analysis using a FACScan flow cytometer. Examples of apoptosis are shown in ( A ). ( B ) Percentages of apoptotis are given as the mean+standard deviation of Log [PI] from three independent experiments. Gray bars indicate HT1080 cells treated by mock infection, doxorubicin, NCV, Ad-wt p53 , Ad- p53 22/23 or Ad- p53 14/19 alone. Black bars indicate cells treated with doxorubicin plus NCV, Ad-wt p53 , Ad- p53 22/23 or Ad- p53 14/19.