DNA Gel Blot Analysis of ac29 Mutants. DNA was isolated from the diploid parent ( nic7 ac29-2 mt − pf14/ ++ mt + +) and the diploid acy9 , acy10 , acy20 , acy25 , and acy32 mutant strains and from the haploid ac29-3 mutant containing the mt + chromosome from acy32 . The DNAs of the blots were digested with BglII (A) or SacI ( [B] and [C] ) and probed with the acB probe ( [A] and [B] ) or the acC probe (C) .