Western blot analysis. Serine phosphorylation and <t>Bcl-2</t> expression in spleen cells cultured in the presence and absence of T-CHO. (a) Serine phosphorylation; (b) Bcl-2 expression after 24 hr in culture and 96 hr in culture. Lanes 1 and 5, spleen cells from pre-exposed mice, cultured in the presence of T-CHO; lanes 2 and 4, cells from T-CHO pre-exposed mice, cultured in the absence T-CHO; lanes 3 and 7, cells from naive mice cultured in the presence of T-CHO and lanes 4 and 8, cells from naive mice cultured in the absence of T-CHO.