NO Tryptophan (Nitrotryptophan) Antibody - Western Blot analysis of 6-Nitrotryptophan-BSA Conjugate showing detection of 67 kDa Nitrotryptophan protein using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 2D12. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: BSA (1 µg). Lane 4: 6-Nitrotryptophan-BSA (0.5 µg). Lane 5: 6-Nitrotryptophan-BSA (1 µg). Lane 6: 7-Ketocholesterol-BSA (0.5 µg). Lane 7: 7-Ketocholesterol-BSA (1 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: Luminol for 1 min. Predicted/Observed Size: 67 kDa.