Lane 1 : HeLa Cell Lysate;\r\nLane 2 : A431 Cell Lysate;\r\nLane 3 : PC12 Cell Lysate;\r\nLane 4 : NIH3T3 Cell Lysate; probed with MEK1 (32G3)\u00a0Monoclonal Antibody, unconjugated (bsm-50303M) at 1:5000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.