( A ) LOX mRNA regulation by GIP depends on PKA. MC3T3 cells were treated with GIP for 4 hours in the presence and absence of PKA pathway activators and inhibitors, and RNA was analyzed for LOX levels. LOX RNA levels were increased by GIP, which was prevented when cells were treated with the adenylyl cyclase inhibitor SQ22536 or the PKA inhibitor PKI 14‐22. Data were calculated using the 2 –(ΔΔCT) method and are represented as fold change relative to GAPDH. Data are means ± SE, *** p