DNAM-1 and NKG2D ligand expression is comparable on csMUC16high expressing OVCAR-3 cells. OVCAR-3 cells were stained for csMUC16 expression, and then NKG2D-Fc or DNAM-1-Fc and an appropriate secondary antibody were added. Cells were initially gated on live single events of tumor cells that were csMUC16low or csMUC16high. The binding of NKG2D-Fc or DNAM-1 to gated csMUC16low (gray line) and csMUC16high (black line) is shown in histogram form. Data is representative of three independent experiments.