Figure 1: Treatment of COS cells with TSA causes accumulation of acetylation on p53, detected by Sandwich ELISA Kit #7236, but does not affect the level of p53 protein, detected by Western analysis. The absorbance at 450 nm is shown in the top figure, while the corresponding Western blot using the Acetylated Lysine mouse mAb (Ac-K-103) #9681 (left panel) or p53 Antibody #2524 (right panel), is shown in the bottom figure.