Mutation or SNO of C144 inhibits oxidant-induced cell death HEK-293 cells expressing either GFP-tagged TRIM72 WT or TRIM72 C144S , or GFP alone were treated for (A) 3 hours or (B) 24 hours with H 2 O 2 (200 μmol/L) with or without GSNO pre-treatment (1 mmol/L, 1 hour). Cell viability was assayed by fluorescent imaging of ethidium bromide staining normalized to cell number (*p