Effect of LATS2 and RRAD of overexpression on A549 cell in vitro A, B . The mRNA levels and protein levels of LATS2 and RRAD in A549 cells transfected with pCDNA–LATS2 or pCDNA-RRAD was detected by qPCR analysis. C, D . MTT assays and Edu staining assays were used to determine the cell viability. Values represent the mean ± s.d. from three independent experiments. E . Cell cycle was analyzed by flow cytometry. The bar chart represents the percentage of cells in G1–G0, S, or G2–M phase, as indicated. *P