IFN-β production in response to Salmonella infection requires TRIF. (A and B) Immortalized macrophages (iMacs) deficient in both MyD88 and TRIF (MyD88 −/− /TRIF −/− ) were pretreated with rapamycin (1 nM) for 1 h (A) or were depleted of FAK by siRNA before incubation with Δ invG Salmonella (B) (MOI, 100) for 5 h. Lysates were immunoblotted with the indicated antibodies; n = 3. (C) Macrophages were treated as described for panels A and B before assessment of IFN-β mRNA levels. IFN-β amounts in infected cells were calculated relative to those seen with uninfected controls. n.s., not significant; n = 3. (D) WT iMacs were depleted of TRIF or MyD88 before pretreatment with rapamycin (1 nM) for 1 h prior to infection with Δ InvG Salmonella for 5 h. Lysates were immunoblotted with the indicated antibodies; n = 3. (E) Macrophages were treated as described for panel D before assessment of IFN-β mRNA levels. IFN-β amounts in infected cells were calculated relative to those seen with uninfected controls. *, P