a Effect of SR proteins down-regulation in VEGF alternative splicing in acidic conditions. siRNA for SF2/ASF, SRp20 and SRp40 (performed in triplicate) were used and its efficacy was demonstrated by real time RT-PCR. b Using siRNA for SF2/ASF, SRp20 and SRp40, there was no shift in the VEGF isoforms pattern observed in acidic conditions, since all isoforms were similar to each other (p > 0.05) and to the control (p > 0.05)