The effect of Sirt6 knockdown on phosphorylation of LKB1 and AMP-activated protein kinase (AMPK) in hepatocytes.(A) Representative western blots for phosphorylated LKB1 at Ser428 (pLKB1), LKB1, phosphorylated AMPKα at Thr172 (pAMPKα), pAMPKα, and β-actin. (B) Densitometric analysis of pLKB1 and pAMPKα. Levels of phosphorylated LKB1 at Ser428 and AMPKα at Thr172 were normalized to values for LKB1 and AMPKα, respectively. FFA, free fatty acids; RGZ, rosiglitazone. Data are means±SEM (n = 6 per group). *p<0.05 vs control (CON; white bar), †p<0.05 vs FFA alone.