Effects of VLFE (A), <30 kDa and >30 kDa fractions of VLFE (B), and VLFEP (C) on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 cells. After 24 h of LPS stimulation, inhibitory effects of VLFE, the <30 kDa and >30 kDa fractions of VLFE and VLFEP on NO production were measured by NO assay. Experiments were performed in triplicate, and data are expressed as mean ± standard error. VLFE, Viscozyme extract of LFE; <30 kDa Fr., <30 kDa fraction of VLFE; >30 kDa Fr., >30 kDa fraction of VLFE; VLFEP, crude polysaccharide of VLFE. ***p < 0.005 vs. LPS-treated cells.