Detection of human and mouse FLIP in 293T cells transfected with a human (lane 2) or mouse FLIP<sub>L</sub> (lane 3) expression plasmid using anti-FLIP, mAb (Dave-2) (AG-20B-0005). Untransfected cells (lane 1). Top arrows indicate full length FLIP, lower arrows indicate cleaved FLIP products. <br /> <strong>Method:</strong> Cell extracts from cells (5x10<sup>4</sup>) transfected with human or mouse FLIP expression plasmid were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-FLIP, mAb (Dave-2) at 1µg/ml. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.