RBM24 inhibits the translation of the core protein by binding to the 5′ TR of pgRNA and blocking 80S ribosome assembly on HBV pgRNA. a The HepG2.2.15 cell line was transfected with siNC or siRBM24 (left panels), HepG2 cells were co-transfected with pHY106 and pRBM24 or empty vector (right panels). Cell lysates were collected at 48 hpt, and the expression of core and RBM24 was detected by western blotting. b HepG2 cells were co-transfected with pHA-core and pRBM24 or empty vector, and the expression of core and RBM24 was detected by western blotting. c HepG2 cells were co-transfected with pTR-core and the indicated siRNA or plasmid and harvested at 48 hpt. The expression of core and RBM24 was detected by western blotting. d HepG2 cells were transfected with the indicated plasmids, and luciferase activity was determined with Steady-Glo ® . The relative luciferase activity (RLA) values were calculated and are shown in the bar graph on the left. The luciferase activity of 5′ TR-associated luciferase reporter plasmids in vitro was detected (bar graph on the right). e The 5′ TR-luciferase RNA together with rhRBM24 or BSA was incubated in rabbit reticulocyte lysate (RRL). The ribosome complexes were separated by sucrose gradient ultracentrifugation. The distribution of biotin-RNA was detected using a dot-blot assay