Liraglutide stimulates PI3K‐dependent AKT signalling in cultured pancreatic βTC‐6 cells. Beta TC‐6 cells grown on 24‐well plates were pre‐treated or not with the PI3K inhibitor, wortmannin for 1 hr and finally stimulated with 1 nmol/l of liraglutide for 3–30 min. Cells were then lysed and AKT‐, BAD‐ and FoxO‐phosphorylation were determined by immunoblotting. Three independent experiments were performed. ( A ) Representative Western blot of (Ser473) AKT phosphorylation. Immunoblots were stripped and re‐probed with anti‐AKT antibody to normalize the blots for protein levels. ( B, C ) Representative Western blots of (Thr24) FoxO1/(Thr32) FoxO3a and (Ser136) BAD phosphorylation. Immunoblots were stripped and re‐probed with anti‐tubulin or anti‐Bad antibody to normalize the blots for protein levels.