A) Typical Luminescence Output from SPARCL Assay Luminescent signal was capture every 0.02 seconds for 2 seconds and produces these representative curves. Samples depicted contain 2875 ng/mL (red), 45 ng/mL (pink) or 0 ng/mL (purple). B) 4-Parameter Fit Curve from 96-well Assay Data calculated using Sum function corresponds to a 4-parameter fit. R2 value = 0.9995.