Figure 1. Western blot analysis of INPPL1  using anti-INPPL1  antibody (A01790). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>Lane 2: human Hela whole cell lysate,<br>Lane 3: human U20S whole cell lysate,<br>Lane 4: human PC-3 whole cell lysate,<br>Lane 5: human Caco-2 whole cell lysate,<br>Lane 6: human A549 whole cell lysate,<br>Lane 7: human K562 whole cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPPL1  antigen affinity purified polyclonal antibody (Catalog # A01790) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INPPL1  at approximately 140-160KD. The expected band size for INPPL1  is at 138KD.