Flotillin expression is required for activation of mouse primary CD4 T cells. a Western blot of splenocyte lysate from WT and flotillin2 KO mice probed with antibodies against flotillin1 and flotillin2. b – e Representative flow cytometry histograms of CD4 T cells isolated from the spleen of WT or flotillin2 KO mice, activated on anti-CD3 and anti-CD28 surfaces for 20 and 36 h and stained with antibodies against CD69 ( b ) or CD25 ( d ). Mean fluorescent intensity (MFI) at the surface of 10,000 cells of WT and flotillin2 KO CD4 T cells stained with antibodies against CD69 ( c ) or CD25 ( e ). Data obtained from three mice for each condition. Error bars indicate mean (±SEM). * p