Integrin α3β1 promotes CD cell adhesion, migration, and proliferation on LM-332 and LM-511. (A) Lysates from Itgα3 −/− and Itgα3 f/f CD cells (20 μg total protein/lane) were immunoblotted for integrin β1, α3, and α6 subunits. β-actin served as a loading control. (B) Surface expression of integrin β1, α3, and α6 subunits was determined on Itgα3 −/− and Itgα3 f/f CD cells by flow cytometry using R-phycoerythrin (PE). (C) Lysates from Itgα3 −/− and Itgα3 f/f CD cells (100 μg total protein) were immunoprecipitated with anti-Itgα6 antibody or normal rabbit IgG and immunoblotted for integrin subunits β1 and β4. Adhesion (D and E), migration (F), and proliferation (G) of Itgα3 f/f and Itgα3 −/− CD cells on LM-332 and LM-511 were evaluated as described in Materials and Methods . Mean measurements ±SEM of four to six independent experiments are shown; *, p ≤ 0.05 between Itgα3 f/f and Itgα3 −/− CD cells. (H) Itgα3 f/f and Itgα3 −/− CD cells were treated with blocking anti-Itgα6 antibody and plated on LM-332. Adhesion was evaluated as described in Materials and Methods . Mean measurements ± SEM of three independent experiments are shown; *, p ≤ 0.05 between CD cells and CD cells treated with blocking anti-Itgα6 antibody.